scholarly journals Fine Mapping of a Clubroot Resistance Gene in Chinese Cabbage Using SNP Markers Identified from Bulked Segregant RNA Sequencing

2017 ◽  
Vol 8 ◽  
Author(s):  
Zhen Huang ◽  
Gary Peng ◽  
Xunjia Liu ◽  
Abhinandan Deora ◽  
Kevin C. Falk ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rna Sequencing ◽  
Chinese Cabbage ◽  
Fine Mapping ◽  
Snp Markers ◽  
Clubroot Resistance ◽  
Clubroot Resistance Gene

Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa

2006 ◽  
Vol 114 (1) ◽  
pp. 81-91 ◽  
Author(s):  
M. Saito ◽  
N. Kubo ◽  
S. Matsumoto ◽  
K. Suwabe ◽  
M. Tsukada ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Brassica Rapa ◽  
Fine Mapping ◽  
Clubroot Resistance ◽  
Clubroot Resistance Gene

TOWARDS THE CLONING OF CLUBROOT RESISTANCE GENE (CRB) IN CHINESE CABBAGE

2006 ◽  
pp. 313-316 ◽  
Author(s):  
Z.Y. Piao ◽  
W.C. Lee ◽  
Y.K. Lee ◽  
H.G. Kim ◽  
J.Y. Jeong ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Chinese Cabbage ◽  
Clubroot Resistance ◽  
Clubroot Resistance Gene

scholarly journals Two Clubroot-Resistance Genes, Rcr3 and Rcr9wa, Mapped in Brassica rapa Using Bulk Segregant RNA Sequencing

2020 ◽  
Vol 21 (14) ◽  
pp. 5033
Author(s):  
Md. Masud Karim ◽  
Abdulsalam Dakouri ◽  
Yan Zhang ◽  
Qilin Chen ◽  
Gary Peng ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rna Sequencing ◽  
Brassica Rapa ◽  
Plasmodiophora Brassicae ◽  
Genetic Resistance ◽  
Doubled Haploid Line ◽  
Resistance Mechanisms ◽  
Snp Markers ◽  
Nucleotide Polymorphisms ◽  
Clubroot Resistance

Genetic resistance is widely used to manage clubroot (Plasmodiophora brassicae) in brassica crops, but new pathotypes have recently been identified on canola (Brassica napus) on the Canadian prairies. Resistance effective against both the most prevalent pathotype (3H, based on the Canadian Clubroot Differential system) and the new pathotypes is needed. BC1 plants of Brassica rapa from a cross of line 96-6990-2 (clubroot resistance originating from turnip cultivar ‘Waaslander’) and a susceptible doubled-haploid line, ACDC, exhibited a 1:1 segregation for resistance against pathotypes 3H and 5X. A resistance gene designated as Rcr3 was mapped initially based on the percentage of polymorphic variants using bulked segregant RNA sequencing (BSR-Seq) and further mapped using Kompetitive Allele Specific PCR. DNA variants were identified by assembling short reads against a reference genome of B. rapa. Rcr3 was mapped into chromosome A08. It was flanked by single nucleotide polymorphisms (SNP) markers (A90_A08_SNP_M12 and M16) between 10.00 and 10.23 Mb, in an interval of 231.6 Kb. There were 32 genes in the Rcr3 interval. Three genes (Bra020951, Bra020974, and Bra020979) were annotated with disease resistance mechanisms, which are potential candidates for Rcr3. Another resistance gene, designated as Rcr9wa, for resistance to pathotype 5X was mapped, with the flanking markers (A90_A08_SNP_M28 and M79) between 10.85 and 11.17 Mb using the SNP sites identified through BSR-Seq for Rcr3. There were 44 genes in the Rcr9wa interval, three of which (Bra020827, Bra020828, Bra020814) were annotated as immune-system-process related genes, which are potential candidates for Rcr9wa.

scholarly journals Fine mapping of the clubroot resistance gene CRb and development of a useful selectable marker in Brassica rapa

2013 ◽  
Vol 63 (1) ◽  
pp. 116-124 ◽  
Author(s):  
Takeyuki Kato ◽  
Katsunori Hatakeyama ◽  
Nobuko Fukino ◽  
Satoru Matsumoto
Keyword(s):  
Resistance Gene ◽  
Brassica Rapa ◽  
Fine Mapping ◽  
Selectable Marker ◽  
Clubroot Resistance ◽  
Clubroot Resistance Gene

scholarly journals Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fuyou Fu ◽  
Xunjia Liu ◽  
Rui Wang ◽  
Chun Zhai ◽  
Gary Peng ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Resistance Gene ◽  
Rna Sequencing ◽  
Catalytic Domain ◽  
Leptosphaeria Maculans ◽  
Snp Markers ◽  
Specific Pcr ◽  
Dual Specificity ◽  
Kinase Catalytic Domain ◽  
Allele Specific

Abstract The fungal pathogen Leptosphaeria maculans causes blackleg disease on canola and rapeseed (Brassica napus) in many parts of the world. A B. napus cultivar, ‘Quinta’, has been widely used for the classification of L. maculans into pathogenicity groups. In this study, we confirmed the presence of Rlm1 in a DH line (DH24288) derived from B. napus cultivar ‘Quinta’. Rlm1 was located on chromosome A07, between 13.07 to 22.11 Mb, using a BC1 population made from crosses of F1 plants of DH16516 (a susceptible line) x DH24288 with bulked segregant RNA Sequencing (BSR-Seq). Rlm1 was further fine mapped in a 100 kb region from 19.92 to 20.03 Mb in the BC1 population consisting of 1247 plants and a F2 population consisting of 3000 plants using SNP markers identified from BSR-Seq through Kompetitive Allele-Specific PCR (KASP). A potential resistance gene, BnA07G27460D, was identified in this Rlm1 region. BnA07G27460D encodes a serine/threonine dual specificity protein kinase, catalytic domain and is homologous to STN7 in predicted genes of B. rapa and B. oleracea, and A. thaliana. Robust SNP markers associated with Rlm1 were developed, which can assist in introgression of Rlm1 and confirm the presence of Rlm1 gene in canola breeding programs.

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