scholarly journals Physiological and Molecular Characterization of Hydroxyphenylpyruvate Dioxygenase (HPPD)-inhibitor Resistance in Palmer Amaranth (Amaranthus palmeri S.Wats.)

2017 ◽  
Vol 8 ◽  
Author(s):  
Sridevi Nakka ◽  
Amar S. Godar ◽  
Prashant S. Wani ◽  
Curtis R. Thompson ◽  
Dallas E. Peterson ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Inhibitor Resistance

scholarly journals First confirmation and characterization of target and non-target site resistance to glyphosate in Palmer amaranth ( Amaranthus palmeri ) from Mexico

2017 ◽  
Vol 115 ◽  
pp. 212-218 ◽  
Author(s):  
Jose Alfredo Dominguez-Valenzuela ◽  
Javid Gherekhloo ◽  
Pablo Tomás Fernández-Moreno ◽  
Hugo Enrique Cruz-Hipolito ◽  
Ricardo Alcántara-de la Cruz ◽  
...  
Keyword(s):  
Palmer Amaranth ◽  
Target Site ◽  
Amaranthus Palmeri ◽  
Target Site Resistance

scholarly journals Comparing responses of sensitive and resistant populations of Palmer amaranth (Amaranthus palmeri) and waterhemp (Amaranthus tuberculatus var. rudis) to PPO inhibitors

2019 ◽  
Vol 34 (1) ◽  
pp. 140-146 ◽  
Author(s):  
Kathryn J. Lillie ◽  
Darci A. Giacomini ◽  
Patrick J. Tranel
Keyword(s):  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Resistant Population ◽  
Evolution Of Resistance ◽  
Amaranthus Tuberculatus ◽  
Inhibitor Resistance ◽  
Dose Responses ◽  
Sensitive Population ◽  
Respective Species ◽  
Better Than

AbstractResistance to protoporphyrinogen oxidase (PPO) inhibitors was first observed in waterhemp in 2001 and was conferred by the deletion of a glycine residue at the 210th position (ΔGly-210) of the PPO enzyme. PPO-inhibitor resistance in Palmer amaranth was first observed in 2011, 10 years later. The objectives of this study were to directly compare PPO inhibitor responses in plants of both species with or without the ΔGly-210 mutation. Using greenhouse experiments, early (EPOST) and late (LPOST) postemergence dose responses using lactofen and fomesafen, and preemergence (PRE) dose responses using fomesafen and flumioxazin, were obtained for a sensitive and resistant population each of waterhemp and Palmer amaranth. An additional spray study confirmed each sensitive population used in the dose responses was representative of its respective species, with regards to PPO-inhibitor sensitivity. When treated at either POST timing, Palmer amaranth was more tolerant than waterhemp, and the ΔGly-210 mutation provided greater resistance in Palmer amaranth (48-fold to >3,440-fold, depending on timing and herbicide) than in waterhemp (31-fold to 123-fold). The level of tolerance in Palmer amaranth was striking; the sensitive Palmer amaranth population treated LPOST survived as well or better than the resistant waterhemp population treated EPOST. With PRE applications, response differences both between species and between resistant and sensitive populations generally were less pronounced, relative to POST applications. Collectively, this research indicates Palmer amaranth tolerance to POST-applied PPO inhibitors could have initially slowed (relative to waterhemp) evolution of resistance to these herbicides, and resistant and sensitive populations of both species are more likely to be effectively controlled with PRE rather than POST applications.

Transfer and Expression of ALS Inhibitor Resistance from Palmer Amaranth (Amaranthus palmeri) to anA. spinosus×A. palmeriHybrid

Weed Science ◽  
2016 ◽  
Vol 64 (2) ◽  
pp. 240-247 ◽  
Author(s):  
William T. Molin ◽  
Vijay K. Nandula ◽  
Alice A. Wright ◽  
Jason A. Bond
Keyword(s):  
Field Experiments ◽  
Acetolactate Synthase ◽  
Palmer Amaranth ◽  
Enzyme Assays ◽  
Amaranthus Palmeri ◽  
Weed Species ◽  
Als Inhibitors ◽  
Inhibitor Resistance ◽  
Als Inhibitor ◽  
Interspecific Transfer

Transfer of herbicide resistance among closely related weed species is a topic of growing concern. A spiny amaranth × Palmer amaranth hybrid was confirmed resistant to several acetolactate synthase (ALS) inhibitors including imazethapyr, nicosulfuron, pyrithiobac, and trifloxysulfuron. Enzyme assays indicated that the ALS enzyme was insensitive to pyrithiobac and sequencing revealed the presence of a known resistance conferring point mutation, Trp574Leu. Alignment of the ALS gene for Palmer amaranth, spiny amaranth, and putative hybrids revealed the presence of Palmer amaranth ALS sequence in the hybrids rather than spiny amaranth ALS sequences. In addition, sequence upstream of the ALS in the hybrids matched Palmer amaranth and not spiny amaranth. The potential for transfer of ALS inhibitor resistance by hybridization has been demonstrated in the greenhouse and in field experiments. This is the first report of gene transfer for ALS inhibitor resistance documented to occur in the field without artificial/human intervention. These results highlight the need to control related species in both field and surrounding noncrop areas to avoid interspecific transfer of resistance genes.

Distribution and validation of genotypic and phenotypic glyphosate and PPO-inhibitor resistance in Palmer amaranth (Amaranthus palmeri) from southwestern Nebraska – ERRATUM

2020 ◽  
pp. 1-1
Author(s):  
Maxwel C Oliveira ◽  
Darci A Giacomini ◽  
Nikola Arsenijevic ◽  
Gustavo Vieira ◽  
Patrick J Tranel ◽  
...  
Keyword(s):  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Inhibitor Resistance

scholarly journals Coevolution of resistance to PPO inhibitors in waterhemp (Amaranthus tuberculatus) and Palmer amaranth (Amaranthus palmeri)

Weed Science ◽  
2019 ◽  
Vol 67 (05) ◽  
pp. 521-526 ◽  
Author(s):  
Kathryn J. Lillie ◽  
Darci A. Giacomini ◽  
Jonathan D. Green ◽  
Patrick J. Tranel
Keyword(s):  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Pcr Assay ◽  
Common Waterhemp ◽  
First Case ◽  
Amaranthus Tuberculatus ◽  
Single Field ◽  
Inhibitor Resistance ◽  
Amino Acid Glycine ◽  
Quantitative Pcr Assay

AbstractThe first case of evolved protoporphyrinogen oxidase (PPO)-inhibitor resistance was observed in 2001 in common waterhemp [Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif]. This resistance in A. tuberculatus is most commonly conferred by deletion of the amino acid glycine at the 210th position (ΔGly-210) of the PPO enzyme (PPO2) encoded by PPX2. In a field in Kentucky in 2015, inadequate control of Amaranthus plants was observed following application of a PPO inhibitor. Morphological observations indicated that survivors included both A. tuberculatus and Palmer amaranth (Amaranthus palmeri S. Watson). Research was conducted to confirm species identities and resistance and then to determine whether resistance evolved independently in the two species or via hybridization. Results from a quantitative PCR assay based on the ribosomal internal transcribed spacer confirmed that both A. tuberculatus and A. palmeri coexisted in the field. The mutation conferring ΔGly-210 in PPO2 was identified in both species; phylogenetic analysis of a region of PPX2, however, indicated that the mutation evolved independently in the two species. Genotyping of greenhouse-grown plants that survived lactofen indicated that all A. tuberculatus survivors, but only a third of A. palmeri survivors, contained the ΔGly-210 mutation. Consequently, A. palmeri plants were evaluated for the presence of an arginine to glycine or methionine substitution at position 128 of PPO2 (Arg-128-Gly and Arg-128-Met). The Arg-128-Gly substitution was found to account for resistance that was not accounted for by the ΔGly-210 mutation in plants from the A. palmeri population. Results from this study provide a modern-day example of both parallel and convergent evolution occurring within a single field.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma
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