scholarly journals Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean

2017 ◽  
Vol 8 ◽  
Author(s):  
Jing Wu ◽  
Jibao Chen ◽  
Lanfen Wang ◽  
Shumin Wang
Keyword(s):  
Transcription Factors ◽  
Drought Stress ◽  
Stress Response ◽  
Common Bean ◽  
Wrky Transcription Factors ◽  
Terminal Drought ◽  
Genome Wide ◽  
Terminal Drought Stress

scholarly journals Genome-Wide Identification and Analysis of the WRKY Gene Family and Cold Stress Response in Acer truncatum

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1867
Author(s):  
Yan Li ◽  
Xiang Li ◽  
Jiatong Wei ◽  
Kewei Cai ◽  
Hongzhi Zhang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Stress Response ◽  
Cold Stress ◽  
Gene Family ◽  
Gene Families ◽  
Regulatory Function ◽  
Wrky Gene ◽  
Wrky Transcription Factors ◽  
Biotic Stresses ◽  
Genome Wide

WRKY transcription factors constitute one of the largest gene families in plants and are involved in many biological processes, including growth and development, physiological metabolism, and the stress response. In earlier studies, the WRKY gene family of proteins has been extensively studied and analyzed in many plant species. However, information on WRKY transcription factors in Acer truncatum has not been reported. In this study, we conducted genome-wide identification and analysis of the WRKY gene family in A. truncatum, 54 WRKY genes were unevenly located on all 13 chromosomes of A. truncatum, the highest number was found in chromosomes 5. Phylogenetic relationships, gene structure, and conserved motif identification were constructed, and the results affirmed 54 AtruWRKY genes were divided into nine subgroup groups. Tissue species analysis of AtruWRKY genes revealed which were differently exhibited upregulation in flower, leaf, root, seed and stem, and the upregulation number were 23, 14, 34, 18, and 8, respectively. In addition, the WRKY genes expression in leaf under cold stress showed that more genes were significantly expressed under 0, 6 and 12 h cold stress. The results of this study provide a new insight the regulatory function of WRKY genes under abiotic and biotic stresses.

scholarly journals Evidence for genotypic differences among elite lines of common bean in the ability to remobilize photosynthate to increase yield under drought

2016 ◽  
Vol 155 (6) ◽  
pp. 857-875 ◽  
Author(s):  
I. M. RAO ◽  
S. E. BEEBE ◽  
J. POLANIA ◽  
M. GRAJALES ◽  
C. CAJIAO ◽  
...  
Keyword(s):  
Drought Stress ◽  
Grain Yield ◽  
Common Bean ◽  
Harvest Index ◽  
Genotypic Differences ◽  
Area Index ◽  
Wild Bean ◽  
Terminal Drought ◽  
Terminal Drought Stress ◽  
Pod Harvest Index

SUMMARYCommon bean (Phaseolus vulgaris L.) is the most important food legume for human consumption. Drought stress is the major abiotic stress limitation of bean yields in smallholder farming systems worldwide. The current work aimed to determine the role of enhanced photosynthate mobilization to improve adaptation to intermittent and terminal drought stress and to identify a few key adaptive traits that can be used for developing drought-resistant genotypes. Field studies were conducted over three seasons at Centro Internacional de Agricultura Tropical, Palmira, Colombia to determine genotypic differences in adaptation to intermittent (two seasons) and terminal (one season) drought stress compared with irrigated conditions. A set of 36 genotypes, including 33 common bean, two wild bean and one cowpea were evaluated using a 6 × 6 lattice design under irrigated and rainfed field conditions. Three common bean elite lines (NCB 226, SEN 56, SER 125) were identified with superior levels of adaptation to both intermittent and terminal drought stress conditions. The greater performance of these lines under drought stress was associated with their ability to remobilize photosynthate to increase grain yield based on higher values of harvest index, pod harvest index, leaf area index and canopy biomass. Two wild bean germplasm accessions (G 19902, G 24390) showed very poor adaptation to both types of drought stress. One small-seeded black line (NCB 226) was superior in combining greater values of canopy biomass with greater ability to mobilize photosynthates to grain under both types of drought stress. Two small-seeded red lines (SER 78, SER 125) seem to combine the desirable traits of enhanced mobilization of photosynthates to seed with effective use of water through canopy cooling under terminal drought stress. Pod harvest index showed significant positive association with grain yield under both types of drought stress and this trait can be used by breeders as an additional selection method to grain yield in evaluation of breeding populations for both types of drought stress.

scholarly journals Genome-wide characterization and expression analysis of citrus NUCLEAR FACTOR-Y (NF-Y) transcription factors identified a novel NF-YA gene involved in drought-stress response and tolerance

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0199187 ◽  
Author(s):  
Suzam L. S. Pereira ◽  
Cristina P. S. Martins ◽  
Aurizangela O. Sousa ◽  
Luciana R. Camillo ◽  
Caroline P. Araújo ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Drought Stress ◽  
Stress Response ◽  
Expression Analysis ◽  
Nuclear Factor ◽  
Nuclear Factor Y ◽  
Genome Wide

scholarly journals A classic approach for determining genomic prediction accuracy under terminal drought stress and well-watered conditions in wheat landraces and cultivars

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247824
Author(s):  
Morteza Shabannejad ◽  
Mohammad-Reza Bihamta ◽  
Eslam Majidi-Hervan ◽  
Hadi Alipour ◽  
Asa Ebrahimi
Keyword(s):  
Drought Stress ◽  
Genomic Selection ◽  
Bread Wheat ◽  
Genomic Prediction ◽  
Prediction Accuracy ◽  
Terminal Drought ◽  
Genome Wide ◽  
Best Linear Unbiased ◽  
Terminal Drought Stress ◽  
Trait Associations

The present study aimed to improve the accuracy of genomic prediction of 16 agronomic traits in a diverse bread wheat (Triticum aestivum L.) germplasm under terminal drought stress and well-watered conditions in semi-arid environments. An association panel including 87 bread wheat cultivars and 199 landraces from Iran bread wheat germplasm was planted under two irrigation systems in semi-arid climate zones. The whole association panel was genotyped with 9047 single nucleotide polymorphism markers using the genotyping-by-sequencing method. A number of 23 marker-trait associations were selected for traits under each condition, whereas 17 marker-trait associations were common between terminal drought stress and well-watered conditions. The identified marker-trait associations were mostly single nucleotide polymorphisms with minor allele effects. This study examined the effect of population structure, genomic selection method (ridge regression-best linear unbiased prediction, genomic best-linear unbiased predictions, and Bayesian ridge regression), training set size, and type of marker set on genomic prediction accuracy. The prediction accuracies were low (-0.32) to moderate (0.52). A marker set including 93 significant markers identified through genome-wide association studies with P values ≤ 0.001 increased the genomic prediction accuracy for all traits under both conditions. This study concluded that obtaining the highest genomic prediction accuracy depends on the extent of linkage disequilibrium, the genetic architecture of trait, genetic diversity of the population, and the genomic selection method. The results encouraged the integration of genome-wide association study and genomic selection to enhance genomic prediction accuracy in applied breeding programs.

scholarly journals GWAS Analysis of Wheat Pre-breeding Germplasm for Terminal Drought Stress Using Next Generation Sequencing Technology

2020 ◽  
Author(s):  
Sajid Shokat ◽  
Deepmala Sehgal ◽  
Fulai Liu ◽  
Sukhwinder Singh
Keyword(s):  
Drought Stress ◽  
Candidate Genes ◽  
Vegetation Index ◽  
Genome Wide Association Study ◽  
Potential Candidate ◽  
Terminal Drought ◽  
Genome Wide ◽  
Population Structure Analysis ◽  
A Genome ◽  
Terminal Drought Stress

Bread wheat (Triticum aestivum L.) is one of the most important cereal crops for food security. Of all the stresses that curtail wheat productivity, drought has the most detrimental effects. Especially terminal drought stress i.e. at the time of flowering imposes a big challenge to sustain grain production. In the current study, 339 pre-breeding lines derived from three-way crosses of exotics x elite lines were evaluated in the irrigated and drought stress environments at Obregon, Mexico for the year 2016 and 2018. Drought significantly reduced yield (Y), spike length (SL), number of grains per spikes (NGS) and thousand kernel weight (TKW) by 46.4, 19.2, 23.5 and 25.9%, respectively in comparison to irrigated conditions. Kernel abortion (KA), highly correlated with Y, increased significantly (11.6%) under drought stress environment. Population structure analysis in this panel revealed three sub-populations and a genome wide linkage disequilibrium (LD) decay was at 2.5 cM. Single marker and haplotypes-based genome wide association study (GWAS) revealed significant associations on three chromosomes; 4A (HB10.7), 2D (HB6.10) and 3B (HB8.12) with Y, SL and TKW, respectively. Likewise, associations on chromosomes 6B (HB17.1) and 3A (HB7.11) were identified for NGS and on 3A (HB7.12) for KA. Five traits i.e. normalized difference vegetation index (NDVI), canopy temperature depression (CTD) days to heading (DTH), NGS, KA were associated at chromosome 3A both under irrigated and drought conditions however, different haplotypes were estimated. Twenty-six SNPs were part of 10 haplotype blocks associated with Y, SL, TKW, NGS and KA. In silico analysis of the associated SNPs/haplotypes showed hits with candidate genes known to confer abiotic stress resistance in model species and crops. Potential candidate genes include those coding for sulfite exporter TauE/SafE family in Arabidopsis thaliana, TBC domain containing protein in Oryza sativa subsp. Japonica and heat shock proteins in Aegilops tauschii subsp. tauschii were revealed. The SNPs linked to the promising genes identified in the study can be used for marker-assisted selection.

scholarly journals Evaluation of Ethiopian Common Bean (Phaseolus vulgaris L.) Genotypes Under Terminal Drought Stress Condition

2021 ◽  
Vol 3 (1) ◽  
pp. 71-78
Author(s):  
Selamawit Abebe Gitore
Keyword(s):  
Drought Stress ◽  
Common Bean ◽  
Analysis Of Variance ◽  
Geometric Mean ◽  
Stress Conditions ◽  
Terminal Drought ◽  
Plant Characteristics ◽  
Growing Conditions ◽  
Terminal Drought Stress ◽  
Water Treatments

The aim of this study was to evaluate twenty-five common bean genotypes for terminal drought stress. Simple lattice designs were used with two replications under stress and non-stress growing conditions on the field. The experiment was performed using irrigation water during the dry season (December-March). Up to flowering, the stress plots were irrigated and the non-stress plots were provided with water up to physiological maturity. Under both stress and non-stress conditions, several plant characteristics related to yield were assessed. The generated data in this study was subject to analysis of variance (ANOVA) using SAS software version 9.0.0. Data from non-stress (NS) and drought stress (DS) treatments were compared to assess the effect of drought stress or water regime on yield-related traits. In order to perform a combined analysis of variance, the datasets from the two treatments were combined (ANOVA). In this experiment, all the genotypes used showed significant differences in yield and some of the components of yield. For all the characteristics measured, except for flowering days, there were substantial variations between the two water treatments. There was no significant correlation between genotypes and water treatments for almost all the traits tested, with the exception of days to flowering, harvest index and root pulling resistance. Genotypes such as SER 125, MALB-67, MALB-65, MALB-51 and MALB-3 performed better under the two water treatments on the basis of mean productivity (MP) and geometric mean (GM). Understanding the relationships between plant characteristics related to drought stress tolerance and their genetic variability for stress-related grain yield, especially terminal water stress conditions, should prompt common bean breeders to take better measurements of yield and more comprehensive features of drought response.

scholarly journals Genome-wide in silico identification, characterization and transcriptional analysis of the family of growth-regulating factors in common bean (Phaseolus vulgaris L.) subjected to polyethylene glycol-induced drought stress

2017 ◽  
Vol 69 (1) ◽  
pp. 5-14 ◽  
Author(s):  
İlker Büyük ◽  
Sümer Aras
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
In Silico ◽  
Expression Patterns ◽  
Transcriptional Analysis ◽  
Genome Wide ◽  
The Common ◽  
Root Tissues

According to most recent findings, growth regulating factors (GRFs) are plant-specific transcription factors (TFs) that play important roles in many processes, including abiotic and biotic stress response mechanisms. Completion of the common bean (Phaseolus vulgaris) genome project has provided researchers with the opportunity to identify all GRF genes in this species. With this aim, a genome-wide in silico study was performed and 10 GRF proteins (called PhvGRFs) were identified in the common bean genome. Conserved and mandatory motifs (QLQ and WRC) were confirmed in all identified PhvGRFs and two segmental duplication events were determined. Most of the PhvGRFs were found to be more similar to Arabidopsis thaliana GRFs than to Zea mays GRFs in a phylogenetic tree. According to the expression analysis of 10 PhvGRFs, inversely related expression patterns were observed in the roots of Yakutiye and Zulbiye cultivars based on their capacity to adopt to drought stress. After drought treatment of the Zulbiye cultivar, a drought-sensitive common bean cultivar, PhvGRF1, PhvGRF2, PhvGRF3, PhvGRF5, PhvGRF6, PhvGRF9 and PhvGRF10 genes were upregulated 2- to 4-fold in root tissues, as compared to the untreated control. The trend of PhvGRF1, PhvGRF2, PhvGRF3, PhvGRF5, PhvGRF6, PhvGRF7, PhvGRF9 and PhvGRF10 genes showed a consistent decline of 2- to 6-fold in root tissues of the drought-tolerant Yakutiye cultivar subjected to 24 h of drought stress. We demonstrated that the expression patterns of the identified PhvGRFs correlated with the drought-stress response in a cultivar-specific manner in the common bean. We suggest that members of the GRF family can also be used for genetic engineering applications in the common bean.

Sign in / Sign up

Export Citation Format

Share Document