scholarly journals Identification of MicroRNAs and Their Target Genes Related to the Accumulation of Anthocyanins in Litchi chinensis by High-Throughput Sequencing and Degradome Analysis

2017 ◽  
Vol 7 ◽  
Author(s):  
Rui Liu ◽  
Biao Lai ◽  
Bing Hu ◽  
Yonghua Qin ◽  
Guibing Hu ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Degradome Analysis ◽  
Litchi Chinensis

Identification of milRNAs and their target genes in Ganoderma lucidum by high-throughput sequencing and degradome analysis

2020 ◽  
Vol 136 ◽  
pp. 103313 ◽  
Author(s):  
Junjie Shao ◽  
Liqiang Wang ◽  
Yang Liu ◽  
Qianru Qi ◽  
Bin Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Ganoderma Lucidum ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Degradome Analysis

scholarly journals Identification of miRNAs and Their Target Genes in Peach (Prunus persica L.) Using High-Throughput Sequencing and Degradome Analysis

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79090 ◽  
Author(s):  
Xiaoyan Luo ◽  
Zhihong Gao ◽  
Ting Shi ◽  
Zongming Cheng ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Prunus Persica ◽  
Degradome Analysis

Uncovering leaf rust responsive miRNAs in wheat (Triticum aestivum L.) using high-throughput sequencing and prediction of their targets through degradome analysis

Planta ◽  
2016 ◽  
Vol 245 (1) ◽  
pp. 161-182 ◽  
Author(s):  
Dhananjay Kumar ◽  
Summi Dutta ◽  
Dharmendra Singh ◽  
Kumble Vinod Prabhu ◽  
Manish Kumar ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Triticum Aestivum L ◽  
Degradome Analysis

scholarly journals Identification of Exogenous Nitric Oxide-Responsive miRNAs from Alfalfa (Medicago sativa L.) under Drought Stress by High-Throughput Sequencing

Genes ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 30
Author(s):  
Yaodong Zhao ◽  
Wenjing Ma ◽  
Xiaohong Wei ◽  
Yu Long ◽  
Ying Zhao ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Drought Stress ◽  
Medicago Sativa ◽  
High Throughput ◽  
Drought Resistance ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Medicago Sativa L

Alfalfa (Medicago sativa L.) is a high quality leguminous forage. Drought stress is one of the main factors that restrict the development of the alfalfa industry. High-throughput sequencing was used to analyze the microRNA (miRNA) profiles of alfalfa plants treated with CK (normal water), PEG (polyethylene glycol-6000; drought stress), and PEG + SNP (sodium nitroprusside; nitric oxide (NO) sprayed externally under drought stress). We identified 90 known miRNAs belonging to 46 families and predicted 177 new miRNAs. Real-time quantitative fluorescent PCR (qRT-PCR) was used to validate high-throughput expression analysis data. A total of 32 (14 known miRNAs and 18 new miRNAs) and 55 (24 known miRNAs and 31 new miRNAs) differentially expressed miRNAs were identified in PEG and PEG + SNP samples. This suggested that exogenous NO can induce more new miRNAs. The differentially expressed miRNA maturation sequences in the two treatment groups were targeted by 86 and 157 potential target genes, separately. The function of target genes was annotated by gene ontology (GO) enrichment and kyoto encyclopedia of genes and genomes (KEGG) analysis. The expression profiles of nine selected miRNAs and their target genes verified that their expression patterns were opposite. This study has documented that analysis of miRNA under PEG and PEG + SNP conditions provides important insights into the improvement of drought resistance of alfalfa by exogenous NO at the molecular level. This has important scientific value and practical significance for the improvement of plant drought resistance by exogenous NO.

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