scholarly journals The Arabidopsis Transcription Factor ANAC032 Represses Anthocyanin Biosynthesis in Response to High Sucrose and Oxidative and Abiotic Stresses

2016 ◽  
Vol 7 ◽  
Author(s):  
Kashif Mahmood ◽  
Zhenhua Xu ◽  
Ashraf El-Kereamy ◽  
José A. Casaretto ◽  
Steven J. Rothstein
Keyword(s):  
Transcription Factor ◽  
Abiotic Stresses ◽  
Anthocyanin Biosynthesis ◽  
High Sucrose

scholarly journals The Transcription Factor AtGLK1 Acts Upstream of MYBL2 to Genetically Regulate Sucrose-Induced Anthocyanin Biosynthesis in Arabidopsis

2021 ◽  
Author(s):  
Dongming Zhao ◽  
Yuxuan Zheng ◽  
lingjun Yang ◽  
Ziyu Yao ◽  
Jianfeng Cheng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stresses ◽  
Transgenic Arabidopsis ◽  
Anthocyanin Biosynthesis ◽  
Negative Regulator ◽  
Transcriptional Level ◽  
Loss Of Function ◽  
Gus Staining ◽  
Real Time Quantitative Pcr ◽  
Plastid Signaling

Abstract Background: The regulation of anthocyanin biosynthesis by various factors including sugars, light and abiotic stresses is mediated by numerous regulatory factors acting at the transcriptional level. Here experimental evidence was provided in order to demonstrate that the nuclear GARP transcription factor AtGLK1 plays an important role in regulating sucrose-induced anthocyanin biosynthesis in Arabidopsis.Results: The results obtained using real-time quantitative PCR and GUS staining assays revealed that AtGLK1 was mainly expressed in the green tissues of Arabidopsis seedlings and could be induced by sucrose. The loss-of-function glk1 glk2 double mutant has lower anthocyanin levels than the glk2 single mutant, although it has been determined that loss of AtGLK1 alone does not affect anthocyanin accumulation. Overexpression of AtGLK1 enhances the accumulation of anthocyanin in transgenic Arabidopsis seedlings accompanied by increased expression of anthocyanin biosynthetic and regulatory genes. Moreover, we found that AtGLK1 also participates in plastid-signaling mediated anthocyanin accumulations. Genetic, physiological, and molecular biological approaches demonstrated that AtGLK1 acts upstream of MYBL2, which is a key negative regulator of anthocyanin biosynthesis, to genetically regulate sucrose-induced anthocyanin biosynthesis.Conclusion: Our results indicated that AtGLK1 positively regulates sucrose-induced anthocyanin biosynthesis in Arabidopsis via MYBL2.

scholarly journals The transcription factor AtGLK1 acts upstream of MYBL2 to genetically regulate sucrose-induced anthocyanin biosynthesis in Arabidopsis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dongming Zhao ◽  
Yuxuan Zheng ◽  
lingjun Yang ◽  
Ziyu Yao ◽  
Jianfeng Cheng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stresses ◽  
Transgenic Arabidopsis ◽  
Anthocyanin Biosynthesis ◽  
Negative Regulator ◽  
Transcriptional Level ◽  
Loss Of Function ◽  
Gus Staining ◽  
Real Time Quantitative Pcr ◽  
Plastid Signaling

Abstract Background The regulation of anthocyanin biosynthesis by various factors including sugars, light and abiotic stresses is mediated by numerous regulatory factors acting at the transcriptional level. Here experimental evidence was provided in order to demonstrate that the nuclear GARP transcription factor AtGLK1 plays an important role in regulating sucrose-induced anthocyanin biosynthesis in Arabidopsis. Results The results obtained using real-time quantitative PCR and GUS staining assays revealed that AtGLK1 was mainly expressed in the green tissues of Arabidopsis seedlings and could be induced by sucrose. The loss-of-function glk1 glk2 double mutant has lower anthocyanin levels than the glk2 single mutant, although it has been determined that loss of AtGLK1 alone does not affect anthocyanin accumulation. Overexpression of AtGLK1 enhances the accumulation of anthocyanin in transgenic Arabidopsis seedlings accompanied by increased expression of anthocyanin biosynthetic and regulatory genes. Moreover, we found that AtGLK1 also participates in plastid-signaling mediated anthocyanin accumulations. Genetic, physiological, and molecular biological approaches demonstrated that AtGLK1 acts upstream of MYBL2, which is a key negative regulator of anthocyanin biosynthesis, to genetically regulate sucrose-induced anthocyanin biosynthesis. Conclusion Our results indicated that AtGLK1 positively regulates sucrose-induced anthocyanin biosynthesis in Arabidopsis via MYBL2.

scholarly journals An R2R3-type myeloblastosis transcription factor MYB103 is involved in phosphorus remobilization

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Fangwei Yu ◽  
Shenyun Wang ◽  
Wei Zhang ◽  
Hong Wang ◽  
Li Yu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Abiotic Stresses ◽  
Myb Transcription Factor ◽  
Ethylene Signaling ◽  
Biotic And Abiotic Stresses ◽  
P Deficiency ◽  
P Concentration ◽  
Increased Sensitivity

Abstract The members of myeloblastosis transcription factor (MYB TF) family are involved in the regulation of biotic and abiotic stresses in plants. However, the role of MYB TF in phosphorus remobilization remains largely unexplored. In the present study, we show that an R2R3 type MYB transcription factor, MYB103, is involved in phosphorus (P) remobilization. MYB103 was remarkably induced by P deficiency in cabbage (Brassica oleracea var. capitata L.). As cabbage lacks the proper mutant for elucidating the mechanism of MYB103 in P deficiency, another member of the crucifer family, Arabidopsis thaliana was chosen for further study. The transcript of its homologue AtMYB103 was also elevated in response to P deficiency in A. thaliana, while disruption of AtMYB103 (myb103) exhibited increased sensitivity to P deficiency, accompanied with decreased tissue biomass and soluble P concentration. Furthermore, AtMYB103 was involved in the P reutilization from cell wall, as less P was released from the cell wall in myb103 than in wildtype, coinciding with the reduction of ethylene production. Taken together, our results uncover an important role of MYB103 in the P remobilization, presumably through ethylene signaling.

scholarly journals Conserved and non-conserved functions of the rice homologs of the Arabidopsis trichome initiation-regulating MBW complex proteins

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Kaijie Zheng ◽  
Xutong Wang ◽  
Yating Wang ◽  
Shucai Wang
Keyword(s):  
Transcription Factor ◽  
Anthocyanin Biosynthesis ◽  
Transcriptional Activator ◽  
Myb Transcription Factor ◽  
Bhlh Transcription Factor ◽  
Seed Color ◽  
Transcription Activator ◽  
Root Hair Formation ◽  
Protoplast Transfection ◽  
Mbw Complex

Abstract Background Trichome initiation in Arabidopsis is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex formed by the R2R3 MYB transcription factor GLABRA1 (GL1), MYB23 or MYB82, the bHLH transcription factor GLABRA3 (GL3), ENHANCER OF GLABRA3 (EGL3) or TRANSPARENT TESTA8 (TT8), and the WD40-repeat protein TRANSPARENT TESTA GLABRA1 (TTG1). However, the functions of the rice homologs of the MBW complex proteins remained uncharacterized. Results Based on amino acid sequence identity and similarity, and protein interaction prediction, we identified OsGL1s, OsGL3s and OsTTG1s as rice homologs of the MBW complex proteins. By using protoplast transfection, we show that OsGL1D, OsGL1E, OsGL3B and OsTTG1A were predominantly localized in the nucleus, OsGL3B functions as a transcriptional activator and is able to interact with GL1 and TTG1. By using yeast two-hybrid and protoplast transfection assays, we show that OsGL3B is able to interact with OsGL1E and OsTTG1A, and OsGL1E and OsTTG1A are also able to interact with GL3. On the other hand, we found that OsGL1D functions as a transcription activator, and it can interact with GL3 but not OsGL3B. Furthermore, our results show that expression of OsTTG1A in the ttg1 mutant restored the phenotypes including alternations in trichome and root hair formation, seed color, mucilage production and anthocyanin biosynthesis, indicating that OsTTG1A and TTG1 may have similar functions. Conclusion These results suggest that the rice homologs of the Arabidopsis MBW complex proteins are able to form MBW complexes, but may have conserved and non-conserved functions.

scholarly journals Global Expressions Landscape of NAC Transcription Factor Family and Their Responses to Abiotic Stresses in Citrullus lanatus

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaolong Lv ◽  
Shanrong Lan ◽  
Kateta Malangisha Guy ◽  
Jinghua Yang ◽  
Mingfang Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stresses ◽  
Citrullus Lanatus ◽  
Nac Transcription Factor ◽  
Transcription Factor Family

The Malus crabapple transcription factor McMYB10 regulates anthocyanin biosynthesis during petal coloration

2014 ◽  
Vol 166 ◽  
pp. 42-49 ◽  
Author(s):  
Rong Jiang ◽  
Ji Tian ◽  
Tingting Song ◽  
Jie Zhang ◽  
Yuncong Yao
Keyword(s):  
Transcription Factor ◽  
Anthocyanin Biosynthesis

An R2R3-MYB transcription factor CmMYB21 represses anthocyanin biosynthesis in color fading petals of chrysanthemum

2022 ◽  
Vol 293 ◽  
pp. 110674
Author(s):  
Yiguang Wang ◽  
Li-Jie Zhou ◽  
Yuxi Wang ◽  
Zhiqiang Geng ◽  
Baoqing Ding ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Anthocyanin Biosynthesis ◽  
Myb Transcription Factor ◽  
Color Fading ◽  
R2r3 Myb

scholarly journals ‘Garnem’ and Myrobalan ‘P.2175’: Two Different Drought Responses and Their Implications in Drought Tolerance

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 299
Author(s):  
Beatriz Bielsa ◽  
María Ángeles Sanz ◽  
María José Rubio-Cabetas
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Abiotic Stresses ◽  
Interspecific Hybrids ◽  
Drought Response ◽  
Breeding Programs ◽  
Wide Range ◽  
Relative Water ◽  
Aba Content ◽  
Rootstock Breeding

One of the challenges in rootstock breeding programs is the combination of tolerances to different abiotic stresses in new interspecific hybrids adapted to a wide range of environmental conditions. In this work, two Prunus L. rootstocks: Myrobalan ‘P.2175’ (P. cerasifera Ehrh.) and the almond × peach hybrid ‘Garnem’ (P. amygdalus Batsch × P. persica (L.) Batsch) were subjected to drought during 24 h to understand their drought response mechanisms. The study was conducted monitoring leaf water potential (LWP), stomatal conductance (gs), relative water content (RWC), and electrolyte leakage (EL); as well as the abscisic acid (ABA) content in roots. The relative expression of five drought-relative genes was also studied. The obtained results allowed examining the drought tolerance potential of ‘Garnem’ and Myrobalan ‘P.2175’, demonstrating the great potential of ‘Garnem’ as drought tolerance source in future selections in breeding. Furthermore, based on the obtained data, the transcription factor Myb25-like could be a good biomarker of drought sensitivity for use in Prunus rootstock breeding programs.

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