scholarly journals Identification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro Culture

2016 ◽  
Vol 7 ◽  
Author(s):  
Zongli Chu ◽  
Junying Chen ◽  
Haixia Xu ◽  
Zhongdong Dong ◽  
Feng Chen ◽  
...  
2001 ◽  
Vol 29 (1-2) ◽  
pp. 127-134 ◽  
Author(s):  
Izabela Marcińska ◽  
Magdalena Szechyńska ◽  
Jolanta Biesaga-Kościelniak ◽  
Maria Filek

2016 ◽  
Vol 32 (5) ◽  
pp. 367-376 ◽  
Author(s):  
M. A. Bannikova ◽  
I. R. Gorbatyuk ◽  
I. S. Hnatiuk ◽  
L. V. Malysheva-Otto ◽  
V. P. Duplij

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).


2012 ◽  
Vol 55 (5) ◽  
pp. 349-360 ◽  
Author(s):  
Yong Chun Li ◽  
Fan Rong Meng ◽  
Chun Yan Zhang ◽  
Ning Zhang ◽  
Ming Shan Sun ◽  
...  

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.


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