scholarly journals Identification of Ice Plant (Mesembryanthemum crystallinum L.) MicroRNAs Using RNA-Seq and Their Putative Roles in High Salinity Responses in Seedlings

2016 ◽  
Vol 7 ◽  
Author(s):  
Chih-Pin Chiang ◽  
Won C. Yim ◽  
Ying-Hsuan Sun ◽  
Miwa Ohnishi ◽  
Tetsuro Mimura ◽  
...  
Keyword(s):  
High Salinity ◽  
Mesembryanthemum Crystallinum ◽  
Rna Seq ◽  
Ice Plant

scholarly journals RNA-Seq Analysis of the Response of the Halophyte, Mesembryanthemum crystallinum (Ice Plant) to High Salinity

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0118339 ◽  
Author(s):  
Hironaka Tsukagoshi ◽  
Takamasa Suzuki ◽  
Kouki Nishikawa ◽  
Sakae Agarie ◽  
Sumie Ishiguro ◽  
...  
Keyword(s):  
High Salinity ◽  
Mesembryanthemum Crystallinum ◽  
Rna Seq ◽  
Ice Plant

scholarly journals Root system architecture analysis in Mesembryanthemum crystallinum (ice plant) seedlings reveals characteristic root halotropic response

Biology Open ◽  
2020 ◽  
pp. bio.052142
Author(s):  
Mayuko Otsuka ◽  
Hikaru Kato ◽  
Shyota Yamada ◽  
Tatsuhiko Nakayama ◽  
Satomi Sakaoka ◽  
...  
Keyword(s):  
Root Growth ◽  
Salinity Stress ◽  
High Salinity ◽  
Growth Traits ◽  
Characteristic Root ◽  
Root System Architecture ◽  
Mesembryanthemum Crystallinum ◽  
Stress Factors ◽  
Plant Roots ◽  
Ice Plant

One of the major environmental stress factors that affect root growth is salinity. Arabidopsis thaliana, a glycophyte, shows halotropism, whereby it alters the direction of root growth in a non-gravitropic pattern to evade high soil salinity. Asymmetric auxin distribution regulated by the relocation of auxin-efflux carrier proteins is a key cellular event in the halotropic response. However, there are no reports of halotropism in halophytes. Here, we investigated root growth traits in Mesembryanthemum crystallinum, ice plant, under high salinity conditions. We hypothesized that ice plant roots would show halotropic responses different from those of Arabidopsis. Notably, similar to halotropism observed in Arabidopsis, ice plant roots showed continuous root bending under salinity stress. However, the root elongation rate did not change in ice plants. Expression analyses of several genes revealed that auxin transport might be partially involved in ice plant halotropism. This study enhances our understanding of halophyte root adaptation to high salinity stress.

scholarly journals Effective Agrobacterium-mediated transformation protocols for callus and roots of halophyte ice plant (Mesembryanthemum crystallinum)

2019 ◽  
Vol 60 (1) ◽  
Author(s):  
Hau-Hsuan Hwang ◽  
Chih-Hao Wang ◽  
Hsiao-Huei Chen ◽  
Jia-Fang Ho ◽  
Shin-Fei Chi ◽  
...  
Keyword(s):  
Mesembryanthemum Crystallinum ◽  
Ice Plant ◽  
Agrobacterium Mediated Transformation

Diurnal expression of five protein phosphatase type 2C genes in the common ice plant, Mesembryanthemum crystallinum

2007 ◽  
Vol 34 (7) ◽  
pp. 581 ◽  
Author(s):  
Ko Sato ◽  
Hiroaki Ohsato ◽  
Shunsuke Izumi ◽  
Saori Miyazaki ◽  
Hans J. Bohnert ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Phosphatase ◽  
Continuous Light ◽  
Mesembryanthemum Crystallinum ◽  
Diurnal Fluctuation ◽  
Mrna Levels ◽  
Ice Plant ◽  
Carboxylase Activity ◽  
The Common ◽  
Protein Phosphatase Type

The common ice plant, Mesembryanthemum crystallinum L., is a eu-halophytic model species with an environmental stress-initiated switch from C3 photosynthesis to crassulacean acid metabolism (CAM). Phosphoenolpyruvate carboxylase activity in 6-week-old plants exposed to salt stress for 5 days was ~15-fold higher than before stress, indicating the salinity-dependent induction of the C3 to CAM transition. Five plant protein phosphatase type 2C (PP2C) genes were cloned, representative of five of the 10 plant PP2C sub-families. We measured mRNA levels of these PP2Cs and of myo-inositol 1-phosphate synthase (Inps1) in 6-week-old plants before (C3) and after (CAM) salt stress. Remarkably, four PP2C genes and Inps1 were expressed with a diurnal fluctuation in plants in C3 mode. After salt-induced CAM induction, the six genes were expressed with more prominent fluctuations than before stress, suggesting that these PP2C genes may be involved in the diurnal regulation of protein phosphorylation in CAM. Under continuous light treatment the expression of two PP2C genes continued to fluctuate, indicating that their expression is controlled by circadian rhythm.

scholarly journals Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum

2021 ◽  
Vol 22 (21) ◽  
pp. 11813
Author(s):  
Ryota Kataoka ◽  
Mami Akashi ◽  
Takeshi Taniguchi ◽  
Yoshiyuki Kinose ◽  
Ahmet Emre Yaprak ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Tricarboxylic Acid Cycle ◽  
Mesembryanthemum Crystallinum ◽  
Initial Growth ◽  
Plant Metabolites ◽  
Ice Plant ◽  
Soil Phytoremediation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mesembryanthemum crystallinum L. (common ice plant) is an edible halophyte. However, if ice plants are used to phytoremediate salinity soil, there are problems of slow initial growth, and a long period before active NaCl uptake occurs under higher salinity conditions. Application of endophytic bacteria may improve the problem, but there remain gaps in our understanding of how endophytic bacteria affect the growth and the biochemical and physiological characteristics of ice plants. The aims of this study were to identify growth-promoting endophytic bacteria from the roots of ice plants and to document the metabolomic response of ice plants after application of selected endophytic bacteria. Two plant growth-promoting endophytic bacteria were selected on the basis of their ability to promote ice plant growth. The two strains putatively identified as Microbacterium spp. and Streptomyces spp. significantly promoted ice plant growth, at 2-times and 2.5-times, respectively, compared with the control and also affected the metabolome of ice plants. The strain of Microbacterium spp. resulted in increased contents of metabolites related to the tricarboxylic acid cycle and photosynthesis. The effects of salt stress were alleviated in ice plants inoculated with the endobacterial strains, compared with uninoculated plants. A deeper understanding of the complex interplay among plant metabolites will be useful for developing microbe-assisted soil phytoremediation strategies, using Mesembryanthemum species.

scholarly journals Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

2020 ◽  
Vol 8 (1) ◽  
pp. 88 ◽  
Author(s):  
Jian Zhang ◽  
Pengcheng Wang ◽  
Hongmei Tian ◽  
Zhen Tao ◽  
Tingting Guo
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Endophytic Bacterium ◽  
Complex Salt ◽  
Rna Seq ◽  
Ice Plant ◽  
Soil Ecosystems ◽  
Plant Growth Promoting ◽  
Microbe Interactions ◽  
Growth Promoting

Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (Mesembryanthemum crystallinum) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB Halomonas sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of Halomonas sp. MC1 to fully understand the dynamics of complex salt–microbe interactions.

Effect of Different CO2 Regimes on the Induction of Crassulacean Acid Metabolism in Mesembryanthemum crystallinum L

1979 ◽  
Vol 6 (6) ◽  
pp. 589 ◽  
Author(s):  
K Winter
Keyword(s):  
Water Stress ◽  
Phosphoenolpyruvate Carboxylase ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
High Salinity ◽  
Stomatal Closure ◽  
Initial Step ◽  
Mesembryanthemum Crystallinum ◽  
Initial Event ◽  
Response To Water

Induction of crassulacean acid metabolism (CAM) in Mesembryanthemum crystallinum in response to high salinity was studied in plants grown in different CO2 regimes to determine whether the induction of CAM could be controlled by CO2 supply in the light and dark; a possible consequence of stomatal closure in response to water stress. The activity of extractable phosphoenolpyruvate carboxylase (EC 4.1.1.31) and the nocturnal change in malate content were followed at frequent intervals after onset of the treatments. The results suggest that the initial event during the induction of CAM is a change in the biochemical apparatus, indicated by the activity of phosphoenolpyruvate carboxylase, which then leads to the day/night fluctuations of malate synthesis typical of CAM. This initial step is not controlled by the availability of CO2 in the light or dark.

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