scholarly journals Identification of microRNAs and Their Target Genes Explores miRNA-Mediated Regulatory Network of Cytoplasmic Male Sterility Occurrence during Anther Development in Radish (Raphanus sativus L.)

2016 ◽  
Vol 7 ◽  
Author(s):  
Wei Zhang ◽  
Yang Xie ◽  
Liang Xu ◽  
Yan Wang ◽  
Xianwen Zhu ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Regulatory Network ◽  
Raphanus Sativus ◽  
Target Genes ◽  
Anther Development ◽  
Raphanus Sativus L

scholarly journals Comparative profile analysis reveals differentially expressed microRNAs regulate anther and pollen development in kenaf cytoplasmic male sterility line

Genome ◽  
2019 ◽  
Vol 62 (7) ◽  
pp. 455-466
Author(s):  
Peng Chen ◽  
Qiqi Shi ◽  
Zhichen Liang ◽  
Hai Lu ◽  
Ru Li
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Regulatory Network ◽  
Pollen Development ◽  
Protein Modification ◽  
Target Genes ◽  
Profile Analysis ◽  
Differentially Expressed ◽  
Auxin Signaling ◽  
Mirna Regulation

Cytoplasmic male sterility (CMS) is advantageous in extensive crop breeding and represents a perfect model for understanding anther and pollen development research. MicroRNAs (miRNAs) play key roles in regulating various biological processes. However, the miRNA-mediated regulatory network in kenaf CMS occurrence remains largely unknown. In the present study, a comparative deep sequencing approach was used to investigate the miRNAs and their roles in regulating anther and pollen development during CMS occurrence. We identified 283 known and 46 new candidate miRNAs in kenaf anther. A total of 67 differentially expressed miRNAs (DEMs) were discovered between CMS and its maintainer line. Among them, 40 and 27 miRNAs were up- and downregulated, respectively. These 67 DEMs were predicted to target 189 genes. Validation of DEMs and putative target genes were confirmed by using real-time quantitative PCR. In addition, a potential miRNA-mediated regulatory network, which mainly involves the auxin signaling pathway, signal transduction, glycolysis and energy metabolism, gene expression, transmembrane transport, protein modification and metabolism, and floral development, that mediates anther development during CMS occurrence was proposed. Taken together, our findings provide a better understanding of the molecular mechanism of miRNA regulation in pollen development and CMS occurrence in kenaf.

Discovery of a novel cytoplasmic male-sterility and its restorer lines in radish (Raphanus sativus L.)

2008 ◽  
Vol 117 (6) ◽  
pp. 905-913 ◽  
Author(s):  
Young-Pyo Lee ◽  
Suhyung Park ◽  
Chaewan Lim ◽  
Hyojung Kim ◽  
Heerae Lim ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Raphanus Sativus ◽  
Raphanus Sativus L

STERILITY IN SUCCESSIVE BACKCROSSES OF RAPHANOBRASSICA (2n = 4x = 36) WITH RECURRENT BRASSICA OLERACEA (2n = 2x = 18)

1979 ◽  
Vol 21 (4) ◽  
pp. 479-485 ◽  
Author(s):  
Gilbert D. McCollum
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Brassica Oleracea ◽  
Chromosome Numbers ◽  
Raphanus Sativus ◽  
Seed Set ◽  
Cole Crops ◽  
Hybrid Plants ◽  
Raphanus Sativus L ◽  
Brassica Oleracea L

A series of crosses to develop cytoplasmic male sterility in the cole crops by transferring the Brassica oleracea L. (2n = 18) genome into the cytoplasm of radish (Raphanus sativus L., 2n = 18) through the amphidiploid Raphanobrassica (2n = 4x = 36) was terminated because of complete seed sterility of the only two BC6 hybrid plants obtained. There was no improvement of seed set in six backcrosses to diploid B. oleracea, and the chromosome numbers of the few plants producing seeds were higher than expected, frequently being close to the triploid number (2n = 3x = 27) of the BC1. Zygotes with less than two radish chromosomes were not found; those with less than four were few; and those with less than six rarely produced offspring.

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