scholarly journals Characterization and Expression Analysis of PtAGL24, a SHORT VEGETATIVE PHASE/AGAMOUS-LIKE 24 (SVP/AGL24)-Type MADS-Box Gene from Trifoliate Orange (Poncirus trifoliata L. Raf.)

2016 ◽  
Vol 7 ◽  
Author(s):  
Lei-Ming Sun ◽  
Jin-Zhi Zhang ◽  
Chun-Gen Hu
2015 ◽  
Vol 43 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Xiaohui WANG ◽  
Junhuan CHENG ◽  
Feng XU ◽  
Xingxiang LI ◽  
Weiwei ZHANG ◽  
...  

As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. To find genes involved in the floral transition of Ginkgo biloba, a MADS-box gene, designated as GbMADS2, was cloned from G. biloba based on EST sequences by RT-PCR. Sequence analysis results showed that the cDNA sequence of GbMADS2 contained a 663 bp length ORF encoding 221 amino acids protein, which displayed typical structure of plant MADS-box protein including MADS, I, and K domains and C terminus. The sequence of GbMADS2 protein was highly homologous to those of MADS-box proteins from other plant species with the highest homologous to AGAMOUS (CyAG) from Cycas revoluta. The phylogenetic tree analysis revealed that GbMADS2 belonged to AGAMOUS clade genes. Real-time PCR analysis indicated that expression levels of GbMADS2 gene in female and male flower were significantly higher than those in root, stem, and leaves, and that GbMADS2 expression level increased along with time of flower development. The spatial and time-course expression profile of GbMADS2 implied that GbMADS2 might be involved in development of reproductive organs. The isolation and expression analysis of GbMADS2 provided basis for further studying the molecular mechanism of flower development in G. biloba.


2013 ◽  
Vol 40 (6) ◽  
pp. 3901-3911 ◽  
Author(s):  
Yongjun Shu ◽  
Diansi Yu ◽  
Dan Wang ◽  
Donglin Guo ◽  
Changhong Guo

2011 ◽  
Vol 28 (2) ◽  
pp. 167-172 ◽  
Author(s):  
Yuri Tanaka ◽  
Tomomichi Yamamura ◽  
Teruhiko Terakawa

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86349 ◽  
Author(s):  
Qingyang Huang ◽  
Wenhua Li ◽  
Ruifeng Fan ◽  
Ying Chang

2006 ◽  
Vol 143 (1) ◽  
pp. 225-235 ◽  
Author(s):  
Ben Trevaskis ◽  
Million Tadege ◽  
Megan N. Hemming ◽  
W. James Peacock ◽  
Elizabeth S. Dennis ◽  
...  

2014 ◽  
Vol 289 (5) ◽  
pp. 903-920 ◽  
Author(s):  
Zongda Xu ◽  
Qixiang Zhang ◽  
Lidan Sun ◽  
Dongliang Du ◽  
Tangren Cheng ◽  
...  

Plant Gene ◽  
2017 ◽  
Vol 12 ◽  
pp. 72-79 ◽  
Author(s):  
Frances C. Sussmilch ◽  
Valérie Hecht ◽  
Jacqueline K. Vander Schoor ◽  
James L. Weller

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1197
Author(s):  
Yujie Zhao ◽  
Honglian Zhao ◽  
Yuying Wang ◽  
Xinhui Zhang ◽  
Xueqing Zhao ◽  
...  

MADS-box is a critical transcription factor regulating the development of floral organs and plays essential roles in the growth and development of floral transformation, flower meristem determination, the development of male and female gametophytes, and fruit development. In this study, 36 MIKC-type MADS-box genes were identified in the ‘Taishanhong’ pomegranate genome. By utilizing phylogenetic analysis, 36 genes were divided into 14 subfamilies. Bioinformatics methods were used to analyze the gene structure, conserved motifs, cis-acting elements, and the protein interaction networks of the MIKC-type MADS-box family members in pomegranate, and their expressions pattern in different tissues of pomegranate were analyzed. Tissue-specific expression analysis revealed that the E-class genes (PgMADS03, PgMADS21, and PgMADS27) were highly expressed in floral tissues, while PgMADS29 was not expressed in all tissues, indicating that the functions of the E-class genes were differentiated. PgMADS15 of the C/D-class was the key gene in the development network of pomegranate flower organs, suggesting that PgMADS15 might play an essential role in the peel and inner seed coat development of pomegranate. The results in this study will provide a reference for the classification, cloning, and functional research of pomegranate MADS-box genes.


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