scholarly journals Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum—Phytophthora capsici Phytopathosystem

2016 ◽  
Vol 7 ◽  
Author(s):  
Chidambareswaren Mahadevan ◽  
Anu Krishnan ◽  
Gayathri G. Saraswathy ◽  
Arun Surendran ◽  
Abdul Jaleel ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Phytophthora Capsici ◽  
Piper Nigrum ◽  
Label Free ◽  
Proteomics Analysis

scholarly journals Comparative Proteomics Analysis of Mouse Habu Nephritis Models with and without Unilateral Nephrectomy

2016 ◽  
Vol 39 (5) ◽  
pp. 1761-1776 ◽  
Author(s):  
Lei Chen ◽  
Yang Lu ◽  
Jun Wen ◽  
Xu Wang ◽  
Lingling Wu ◽  
...  
Keyword(s):  
Renal Injury ◽  
Quantitative Proteomics ◽  
Mesangial Cells ◽  
Unilateral Nephrectomy ◽  
Label Free ◽  
Proteomics Analysis ◽  
Single Kidney ◽  
Regulation Of Actin Cytoskeleton ◽  
Insight Into

Background/Aims: Individuals possessing a single kidney are at greater risk of renal injury upon exposure to harmful stimuli. This study aimed to explore the pathogenesis of renal injury in glomerulonephritis with versus without unilateral nephrectomy (UNX). Methods: Histological analysis and label-free quantitative proteomics were performed on two models—the Habu snake venom-induced glomerulonephritis model with versus without UNX (HabuU and Habu models, respectively). The role of villin 1, a differentially expressed protein (DEP) in mouse mesangial cells, was investigated. Results: Persistent mesangiolysis and focal hypercellularity together with reduced activation of cell proliferation in the HabuU model induced more serious renal injury compared with that in the Habu model. The DEPs between the two models were identified by label-free liquid chromatography-mass spectrometry. The KEGG pathway results indicated that regulation of actin cytoskeleton and focal adhesion were specifically enriched in the HabuU model. The cytoskeleton regulation protein villin 1 was downregulated in the HabuU model, but unchanged in the Habu model. Knockdown of villin 1 promoted apoptosis and inhibited the proliferation of mouse mesangial cells, suggesting villin 1 to be involved in qlomerular lesion self-repair insufficiency. Conclusion: By assessing the proteomic profiles of the two models, this study identified several important differences, particularly villin 1 expression, in regulatory mechanisms between the two models. Our findings provide novel insight into the mechanism of serious renal injury in glomerulonephritis with UNX.

scholarly journals The effect of lipocalin-2 (LCN2) on apoptosis: a proteomics analysis study in an LCN2 deficient mouse model

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Dongming Wu ◽  
Xiaopeng Wang ◽  
Ye Han ◽  
Yayun Wang
Keyword(s):  
Knockout Mice ◽  
Quantitative Proteomics ◽  
Energy Homeostasis ◽  
Metabolic Diseases ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Lipocalin 2 ◽  
Wild Type ◽  
Proteomics Analysis ◽  
Associated Proteins

Abstract Background Recent studies have shown that lipocalin-2 (LCN2) has multiple functions involved in various biological and pathological processes including energy homeostasis, cancer, inflammation, and apoptosis. We aimed to investigate the effect of LCN2 on apoptosis that influences the pathogenetic process of metabolic diseases and cancer. Methods We performed a proteomics analysis of livers taken from LCN2-knockout mice and wild type mice by using label-free LC-MS/MS quantitative proteomics. Results Proteomic analysis revealed that there were 132 significantly differentially expressed proteins (49 upregulated and 83 downregulated) among 2140 proteins in the liver of LCN2-knockout mice compared with wild type mice. Of these, seven apoptosis-associated proteins were significantly upregulated and seven apoptosis-associated proteins downregulated. Conclusion Proteomics demonstrated that there were seven upregulated and seven downregulated apoptosis-associated proteins in liver of LCN2-knockout mice. It is important to clarify the effect of LCN2 on apoptosis that might contribute to the pathogenesis of insulin resistance, cancer, and various nervous system diseases.

Label-free quantitative proteomics analysis reveals distinct molecular characteristics in endocardial endothelium

2018 ◽  
Vol 451 (1-2) ◽  
pp. 1-10 ◽  
Author(s):  
Abdul Jaleel ◽  
A. Aneesh Kumar ◽  
G. S. Ajith Kumar ◽  
Arun Surendran ◽  
Chandrashekaran C. Kartha
Keyword(s):  
Quantitative Proteomics ◽  
Molecular Characteristics ◽  
Label Free ◽  
Proteomics Analysis ◽  
Endocardial Endothelium

scholarly journals Label-free quantitative proteomics analysis of jujube (Ziziphus jujuba Mill.) during different growth stages

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22106-22119
Author(s):  
Xiaoli Huang ◽  
Zhaohua Hou
Keyword(s):  
Quantitative Proteomics ◽  
Growth Stage ◽  
Growth Stages ◽  
Label Free ◽  
Flavor Quality ◽  
Chinese Jujube ◽  
Proteomics Analysis ◽  
Zizyphus Jujuba ◽  
Ziziphus Jujuba ◽  
Different Growth Stages

Chinese jujube (Zizyphus jujuba Mill.), a member of the Rhamnaceae family with favorable nutritional and flavor quality, exhibited characteristic climacteric changes during its fruit growth stage.

Label-Free Quantitative Proteomics Analysis of the Sorafenib Resistance in HepG2 Cells

2021 ◽  
Author(s):  
Zi-Xuan Wang ◽  
Hong-Wei Chu ◽  
Kai-Guang Yang ◽  
Bao-Feng Zhao ◽  
Zhen Liang ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Quantitative Proteomics ◽  
Label Free ◽  
Proteomics Analysis

Label-Free Quantitative Proteomics Analysis of Antibiotic Response inStaphylococcus aureusto Oxacillin

2014 ◽  
Vol 13 (3) ◽  
pp. 1223-1233 ◽  
Author(s):  
Xiaofen Liu ◽  
Yingwei Hu ◽  
Pei-Jing Pai ◽  
Daijie Chen ◽  
Henry Lam
Keyword(s):  
Quantitative Proteomics ◽  
Label Free ◽  
Proteomics Analysis ◽  
Antibiotic Response
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