scholarly journals Identification and Characterization of the Sucrose Synthase 2 Gene (Sus2) in Durum Wheat

2016 ◽  
Vol 7 ◽  
Author(s):  
Mariateresa Volpicella ◽  
Immacolata Fanizza ◽  
Claudia Leoni ◽  
Agata Gadaleta ◽  
Domenica Nigro ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Sucrose Synthase ◽  
Identification And Characterization

Identification and characterization of the Populus sucrose synthase gene family

Gene ◽  
2014 ◽  
Vol 539 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Xinmin An ◽  
Zhong Chen ◽  
Jingcheng Wang ◽  
Meixia Ye ◽  
Lexiang Ji ◽  
...  
Keyword(s):  
Gene Family ◽  
Sucrose Synthase ◽  
Identification And Characterization

Identification and characterization of durum wheat microRNAs in leaf and root tissues

2017 ◽  
Vol 17 (5) ◽  
pp. 583-598 ◽  
Author(s):  
Veronica Fileccia ◽  
Edoardo Bertolini ◽  
Paolo Ruisi ◽  
Dario Giambalvo ◽  
Alfonso Salvatore Frenda ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Root Tissues ◽  
Identification And Characterization

scholarly journals HRM technology for the identification and characterization of INDEL and SNPs mutations in genes involved in drought and salt tolerance of durum wheat

2011 ◽  
Vol 9 (2) ◽  
pp. 166-169 ◽  
Author(s):  
Linda Mondini ◽  
Miloudi M. Nachit ◽  
Enrico Porceddu ◽  
Mario A. Pagnotta
Keyword(s):  
Durum Wheat ◽  
Abiotic Stress Tolerance ◽  
Nucleotide Polymorphisms ◽  
Specific Primers ◽  
Single Nucleotide ◽  
Drought And Salt Tolerance ◽  
Pcr Products ◽  
Identification And Characterization ◽  
Single Snps

WRKY transcription factors are one of the largest families of transcriptional regulators and form an integral part of signalling webs which modulate many plant processes, such as abiotic stress tolerance. In the present paper, an innovative method has been applied to identify novel WRKY-1 alleles involved in the responses to salt and drought stresses in Triticum durum. This technique involves scanning for sequencing variations in cDNA-derived PCR amplicons, using high-resolution melting (HRM) followed by direct Sanger sequencing of only those amplicons which were predicted to carry nucleotide changes. HRM represents a novel advance in detection of single-nucleotide polymorphisms (SNPs) by measuring temperature-induced strand separation of short PCR amplicons. The use of this approach is still limited in the field of plant biology. Here, HRM analysis has been applied to the discovery and genotyping of durum wheat SNPs. Specific primers have been designed, starting at multi-alignment of WRKY-1-conserved portions. The PCR amplicons, containing single SNPs, produce distinctive HRM profiles, and by sequencing the PCR products identified, SNPs have been characterized and validated. The results showed that all the revealed SNPs are located on salt-tolerant varieties, confirming their value in breeding activities.

scholarly journals Identification and characterization of the duplicate rice sucrose synthase genes OsSUS5 and OsSUS7 which are associated with the plasma membrane

2011 ◽  
Vol 31 (6) ◽  
pp. 553-561 ◽  
Author(s):  
Jung-Il Cho ◽  
Hyun-Bi Kim ◽  
Chi-Yeol Kim ◽  
Tae-Ryong Hahn ◽  
Jong-Seong Jeon
Keyword(s):  
Plasma Membrane ◽  
Sucrose Synthase ◽  
Identification And Characterization
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