scholarly journals Loss-of-Function Mutations in CsMLO1 Confer Durable Powdery Mildew Resistance in Cucumber (Cucumis sativus L.)

2015 ◽  
Vol 6 ◽  
Author(s):  
Jingtao Nie ◽  
Yunli Wang ◽  
Huanle He ◽  
Chunli Guo ◽  
Wenying Zhu ◽  
...  
2013 ◽  
Vol 126 (8) ◽  
pp. 2149-2161 ◽  
Author(s):  
Xiaoming He ◽  
Yuhong Li ◽  
Sudhakar Pandey ◽  
Brain S. Yandell ◽  
Mamta Pathak ◽  
...  

2005 ◽  
Vol 112 (2) ◽  
pp. 243-250 ◽  
Author(s):  
Y. Sakata ◽  
N. Kubo ◽  
M. Morishita ◽  
E. Kitadani ◽  
M. Sugiyama ◽  
...  

2006 ◽  
Vol 112 (5) ◽  
pp. 986-986 ◽  
Author(s):  
Y. Sakata ◽  
N. Kubo ◽  
M. Morishita ◽  
E. Kitadani ◽  
M. Sugiyama ◽  
...  

1992 ◽  
Vol 117 (3) ◽  
pp. 500-503 ◽  
Author(s):  
Steven P.C. Groot ◽  
Sierd Zijlstra ◽  
Johannes Jansen

Powdery mildew-resistant (PMR) cultivars of cucumber (Cucumis sativus L.) grown in greenhouses in the Netherlands during winter show chlorosis on the full-grown leaves of the main stem. The symptoms are yellowing and occasional necrosis between the main veins of the leaves, resembling symptoms of P toxicity. Severity of the chlorosis may vary from one winter to another. Variation is also observed in the severity of the symptoms between cultivars and individual plants of a cultivar. High P nutrition results in an increase of the severity of chlorosis and provides a better discriminating environment for the selection of PMR genotypes that are less susceptible for leaf chlorosis.


2008 ◽  
Vol 21 (1) ◽  
pp. 30-39 ◽  
Author(s):  
Yuling Bai ◽  
Stefano Pavan ◽  
Zheng Zheng ◽  
Nana F. Zappel ◽  
Anja Reinstädler ◽  
...  

The resistant cherry tomato (Solanum lycopersicum var. cerasiforme) line LC-95, derived from an accession collected in Ecuador, harbors a natural allele (ol-2) that confers broad-spectrum and recessively inherited resistance to powdery mildew (Oidium neolycopersici). As both the genetic and phytopathological characteristics of ol-2–mediated resistance are reminiscent of powdery mildew immunity conferred by loss-of-function mlo alleles in barley and Arabidopsis, we initiated a candidate-gene approach to clone Ol-2. A tomato Mlo gene (SlMlo1) with high sequence-relatedness to barley Mlo and Arabidopsis AtMLO2 mapped to the chromosomal region harboring the Ol-2 locus. Complementation experiments using transgenic tomato lines as well as virus-induced gene silencing assays suggested that loss of SlMlo1 function is responsible for powdery mildew resistance conferred by ol-2. In progeny of a cross between a resistant line bearing ol-2 and the susceptible tomato cultivar Moneymaker, a 19-bp deletion disrupting the SlMlo1 coding region cosegregated with resistance. This polymorphism results in a frameshift and, thus, a truncated nonfunctional SlMlo1 protein. Our findings reveal the second example of a natural mlo mutant that possibly arose post-domestication, suggesting that natural mlo alleles might be evolutionarily short-lived due to fitness costs related to loss of mlo function.


2011 ◽  
Vol 12 (9) ◽  
pp. 866-878 ◽  
Author(s):  
MATT HUMPHRY ◽  
ANJA REINSTÄDLER ◽  
SERGEY IVANOV ◽  
TON BISSELING ◽  
RALPH PANSTRUGA

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