scholarly journals The role of dynamic instability in microtubule organization

2014 ◽  
Vol 5 ◽  
Author(s):  
Tetsuya Horio ◽  
Takashi Murata
1995 ◽  
Vol 349 (1329) ◽  
pp. 297-297

Many cellular mechanisms use a process of variation and selection to generate specific patterns. Among these, dynamic instability of microtubules has been shown to employ a specific mechanism to intentionally generate variation. In many systems the growth of neurons or neuronal processes is excessive, the final connections being established by stabilization of functional interactions. When changes in neuronal networks take place, such as in metamorphosis, use is made of the plasticity of neuronal connectivity. In the immune system, specific responses are generated by variation and selection. Processes that explore a wide range of conditions and a wide range of structures can be called exploratory processes. These are very robust and capable of responding to damage, variability in the environment and ontogenic changes in the organisms. Such robustness would be useful for adapting to changes that occur during phylogenetic changes as well. Given the extensive history of extinction and radiation in evolution, it may be supposed that these mechanisms have themselves been selected for their capacity to survive rapid changes in the organism and for their ability to generate cellular variation.


Author(s):  
C. J. Teo ◽  
Z. S. Spakovszky

One of the major challenges for the successful operation of high-power-density micro-devices lies in the stable operation of the bearings supporting the high-speed rotating turbomachinery. Previous modeling efforts by Piekos [1], Liu et al. [2] and Spakovszky and Liu [3] have mainly focused on the operation and stability of journal bearings. However, since thrust bearings play the vital role of providing axial support and stiffness, there is a need to gain a fuller understanding of their behavior. In this work, a rigorous theory is presented to analyze the effects of compressibility in micro-flows (characterized by low Reynolds numbers and high Mach numbers) through hydrostatic thrust bearings for application to microturbomachines. The analytical model, which combines a 1-D compressible flow model with Finite-Element Analysis, serves as a useful tool for establishing operating protocols and assessing the stability characteristics of hydrostatic thrust bearings. The model is capable of predicting key steady-state performance indicators, such as bearing mass flow, axial stiffness and natural frequency as a function of the hydrostatic supply pressure and thrust bearing geometry. The model has been applied to investigate the static stability of hydrostatic thrust bearings in micro-turbine-generators, where the electrostatic attraction between the stator and rotor gives rise to a negative axial stiffness contribution and may lead to device failure. Thrust bearing operating protocols have been established for a micro-turbopump, where the bearings also serve as an annular seal preventing the leakage of pressurized liquid from the pump to the gaseous flow in the turbine. The dual role of the annular pad poses challenges in the operation of both the device and the thrust bearing. The operating protocols provide essential information for the required thrust bearing supply pressures and axial gaps required to prevent the leakage of water into the thrust bearings for various pump outlet pressures. Good agreement is observed between the model predictions and experimental results. In addition, a dynamic stability analysis is also performed, which indicates the occurrence of unstable axial oscillations due to flow choking effects in both forward and aft thrust bearings. These a-priori dynamic stability predictions were subsequently verified experimentally on a micro-turbocharger. The frequencies of unstable axial oscillations predicted using the model compare favorably to those determined experimentally, thus vindicating the validity of the model. A simple and useful dynamic stability criterion is established, where the occurrence of flow choking in both thrust bearings give rise to dynamic instability.


2010 ◽  
Vol 4 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Masami Yamada ◽  
Shinji Hirotsune ◽  
Anthony Wynshaw-Boris

2011 ◽  
Vol 22 (7) ◽  
pp. 1025-1034 ◽  
Author(s):  
Rajat Bhattacharya ◽  
Hailing Yang ◽  
Fernando Cabral

A multigene family produces tubulin isotypes that are expressed in a tissue-specific manner, but the role of these isotypes in microtubule assembly and function is unclear. Recently we showed that overexpression or depletion of β5-tubulin, a minor isotype with wide tissue distribution, inhibits cell division. We now report that elevated β5-tubulin causes uninterrupted episodes of microtubule shortening and increased shortening rates. Conversely, depletion of β5-tubulin reduces shortening rates and causes very short excursions of growth and shortening. A tubulin conformation-sensitive antibody indicated that the uninterrupted shortening can be explained by a relative absence of stabilized patches along the microtubules that contain tubulin in an assembly-competent conformation and normally act to restore microtubule growth. In addition to these changes in dynamic instability, overexpression of β5-tubulin causes fragmentation that results from microtubule detachment from centrosomes, and it is this activity that best explains the effects of β5 on cell division. Paclitaxel inhibits microtubule detachment, increases the number of assembly-competent tubulin patches, and inhibits microtubule shortening, thus providing an explanation for why the drug can counteract the phenotypic effects of β5 overexpression. On the basis of these observations, we propose that cells can use β5-tubulin expression to adjust the behavior of the microtubule cytoskeleton.


2017 ◽  
Author(s):  
Katja M. Taute ◽  
Ernst-Ludwig Florin

ABSTRACTMany eukaryotic cell functions depend on dynamic instability, meaning the nucleotide-driven assembly and disassembly of microtubules. Assembly requires the constituent tubulin dimers to bind the nucleotide GTP, and its subsequent hydrolysis to GDP induces disassembly. The underlying structural mechanisms, however, are not well understood. Here, we determine the strength of contacts in the microtubule lattice by combining high precision measurements of the bending stiffness of analogues of GTP and GDP microtubules with a recent theoretical model. While previous structural studies have focussed on how the curvature of the tubulin dimer is affected by nucleotide binding, we present evidence of a dramatic regulation of the lateral interactions between the parallel protofilaments that dimers form in the microtubule. We conclude that the shear coupling between neighboring protofilaments is at least two orders of magnitude stronger in the GTP state than in the GDP state, and discuss the implications for the microtubule assembly.


Author(s):  
Eileen O'Toole ◽  
Garrett Greenan ◽  
Karen I. Lange ◽  
Martin Srayko ◽  
Thomas Müller-Reichert

Zygote ◽  
2005 ◽  
Vol 13 (3) ◽  
pp. 219-226 ◽  
Author(s):  
Tomoya Kotani ◽  
Masakane Yamashita

Vertebrate oocytes do not contain centrosomes and therefore form an acentrosomal spindle during oocyte maturation. γ-Tubulin is known to be essential for nucleation of microtubules at centrosomes, but little is known about the behaviour and role of γ-tubulin during spindle formation in oocytes. We first observed sequential localization of γ-tubulin during spindle formation in Xenopus oocytes. γ-Tubulin assembled in the basal regions of the germinal vesicle (GV) at the onset of germinal vesicle breakdown (GVBD) and remained on the microtubule-organizing centre (MTOC) until a complex of the MTOC and transient-microtubule array (TMA) reached the oocyte surface. Prior to bipolar spindle formation, oocytes formed an aggregation of microtubules and γ-tubulin was concentrated at the centre of the aggregation. At the late stage of bipolar spindle formation, γ-tubulin accumulated at each pole. Anti-dynein antibody disrupted the localization of γ-tubulin, indicating that the translocation described above is dependent on dynein activity. We finally revealed that XMAP215, a microtubule-associated protein cooperating with γ-tubulin for the assembly of microtubules, but not γ-tubulin, was phosphorylated during oocyte maturation. These results suggest that γ-tubulin is translocated by dynein to regulate microtubule organization leading to spindle formation and that modification of the molecules that cooperate with γ-tubulin, but not γ-tubulin itself, is important for microtubule reorganization.


2018 ◽  
Vol 503 (2) ◽  
pp. 1160-1167 ◽  
Author(s):  
Kazunori Kume ◽  
Sayuri Kaneko ◽  
Kenji Nishikawa ◽  
Masaki Mizunuma ◽  
Dai Hirata

1999 ◽  
Vol 147 (6) ◽  
pp. 1261-1274 ◽  
Author(s):  
Shuo Ma ◽  
Leda Triviños-Lagos ◽  
Ralph Gräf ◽  
Rex L. Chisholm

Cytoplasmic dynein intermediate chain (IC) mediates dynein–dynactin interaction in vitro (Karki, S., and E.L. Holzbaur. 1995. J. Biol. Chem. 270:28806–28811; Vaughan, K.T., and R.B. Vallee. 1995. J. Cell Biol. 131:1507–1516). To investigate the physiological role of IC and dynein–dynactin interaction, we expressed IC truncations in wild-type Dictyostelium cells. ICΔC associated with dynactin but not with dynein heavy chain, whereas ICΔN truncations bound to dynein but bound dynactin poorly. Both mutations resulted in abnormal localization to the Golgi complex, confirming dynein function was disrupted. Striking disorganization of interphase microtubule (MT) networks was observed when mutant expression was induced. In a majority of cells, the MT networks collapsed into large bundles. We also observed cells with multiple cytoplasmic asters and MTs lacking an organizing center. These cells accumulated abnormal DNA content, suggesting a defect in mitosis. Striking defects in centrosome morphology were also observed in IC mutants, mostly larger than normal centrosomes. Ultrastructural analysis of centrosomes in IC mutants showed interphase accumulation of large centrosomes typical of prophase as well as unusually paired centrosomes, suggesting defects in centrosome replication and separation. These results suggest that dynactin-mediated cytoplasmic dynein function is required for the proper organization of interphase MT network as well as centrosome replication and separation in Dictyostelium.


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