scholarly journals Loss of the ETR1 ethylene receptor reduces the inhibitory effect of far-red light and darkness on seed germination of Arabidopsis thaliana

2014 ◽  
Vol 5 ◽  
Author(s):  
Rebecca L. Wilson ◽  
Arkadipta Bakshi ◽  
Brad M. Binder
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Red Light ◽  
Inhibitory Effect ◽  
Ethylene Receptor

scholarly journals The counteracting effect of potassium cyanide in sodium azide-inhibited germination of Paulownia tomentosa  Steud. seeds

2005 ◽  
Vol 57 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Suzana Zivkovic ◽  
Dragoljub Grubisic ◽  
Zlatko Giba ◽  
Radomir Konjevic
Keyword(s):  
Seed Germination ◽  
Light Pulse ◽  
Sodium Azide ◽  
Incubation Medium ◽  
Red Light ◽  
Potassium Cyanide ◽  
Inhibitory Effect ◽  
Escape Time ◽  
Paulownia Tomentosa ◽  
Respiratory Inhibitors

The effect of some respiratory inhibitors on light-induced Paulownia tomentosa Steud. seed germination was studied. Millimolar solution of sodium azide was sufficient to completely prevent germination induced by a 5-min red light pulse. The inhibitory effect of azide was absent if seeds were rinsed before phytochrome activation by light. Sodium azide was effective only if present in the period of Pfr activity. The escape time from azide inhibition compared to the escape from far-red light action, was delayed for about 24 hours. When azide was applied after phytochrome activation, its effect depended on how long it was present in the incubation medium. The removal of azide allowed full restoration of germination by another red light pulse and the far-red escape time did not differ from the escape of untreated, i.e. water-imbibed seeds. Potassium cyanide alone did not produce any effect in light-stimulated germination of these seeds. However, it counteracted the inhibitory effect of azide in light-stimulated germination, if applied simultaneously at a concentration three times higher.

scholarly journals Arabidopsis chloroplast J protein DJC75/CRRJ mediates nitrate-promoted seed germination in the dark

2020 ◽  
Vol 125 (7) ◽  
pp. 1091-1099
Author(s):  
Huai-Syuan Ciou ◽  
Yu-Lun Tsai ◽  
Chi-Chou Chiu
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Germination Rate ◽  
Red Light ◽  
Phytochrome B ◽  
Biosynthetic Gene ◽  
Unknown Mechanism ◽  
J Domain ◽  
Domain Protein ◽  
J Protein

Abstract Background and Aims Nitrate can stimulate seed germination of many plant species in the absence of light; however, the molecular mechanism of nitrate-promoted seed germination in the dark remains largely unclear and no component of this pathway has been identified yet. Here, we show that a plastid J-domain protein, DJC75/CRRJ, in arabidopsis (Arabidopsis thaliana) is important for nitrate-promoted seed germination in the dark. Methods The expression of DJC75 during imbibition in the dark was investigated. The seed germination rate of mutants defective in DJC75 was determined in the presence of nitrate when light cues for seed germination were eliminated by the treatment of imbibed seeds with a pulse of far-red light to inactivate phytochrome B (phyB), or by assaying germination in the dark with seeds harbouring the phyB mutation. The germination rates of mutants defective in CRRL, a J-like protein related to DJC75, and in two chloroplast Hsp70s were also measured in the presence of nitrate in darkness. Key Results DJC75 was expressed during seed imbibition in the absence of light. Mutants defective in DJC75 showed seed germination defects in the presence of nitrate when light cues for seed germination were eliminated. Mutants defective in CRRL and in two chloroplast Hsp70s also exhibited similar seed germination defects. Upregulation of gibberellin biosynthetic gene GA3ox1 expression by nitrate in imbibed phyB mutant seeds was diminished when DJC75 was knocked out. Conclusions Our data suggest that plastid J-domain protein DJC75 regulates nitrate-promoted seed germination in the dark by upregulation of expression of the gibberellin biosynthetic gene GA3ox1 through an unknown mechanism and that DJC75 may work in concert with chloroplast Hsp70s to regulate nitrate-promoted seed germination. DJC75 is the first pathway component identified for nitrate-promoted seed germination in the dark.

Characterization of SAN 9789-Stimulated Lettuce (Lactuca sativa) Seed Germination

Weed Science ◽  
1985 ◽  
Vol 33 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Karl-Olof Widell ◽  
Christer Sundqvist ◽  
Hemming I. Virgin
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Lactuca Sativa ◽  
Red Light ◽  
Inhibitory Effect ◽  
Lettuce Seeds ◽  
Grand Rapids ◽  
Minor Degree ◽  
A Minor

Dark germination of light-requiring lettuce seeds (Lactuca sativaL. ‘Grand Rapids’) was stimulated by SAN 9789 [4-chloro-5-(methylamino-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone] and to a minor degree by BASF 13761 [4-chloro-5-methoxy-2-phenyl-3(2H)-pyridazinone] and BASF 44521 [4-chloro-5-methoxy-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], but not by’ pyrazon [5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone], SAN 9785 [4-chloro-5-(dimethylamino)-2-phenyl-3 (2H)-pyridazinone], SAN 9774 [5-amino-4-chloro-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], or SAN 6706 [4-chloro-5-(dimethylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone]. SAN 9789 stimulation was inhibited by cis-4-cyclohexene-1,2-dicarboximide (CHDC), and abscisic acid (ABA) at 1 × 10-4M. Red light nullified the inhibitory effect of CHDC (1 × 10-4M) but not the inhibitory effect of ABA (1 × 10-4M) on SAN 9789 stimulated germination. Gibberellic acid (GA3) and kinetin (6-furfurylaminopurine) increased the germination stimulatory effect of SAN 9789 in darkness. Temperatures above 25 C decreased the effect of SAN 9789, with a temperature of 35 C completely inhibiting germination. The inhibitory effect of CHDC was strongly decreased at temperatures below 20 C. SAN 9789-induced germination in darkness was always the same (25 to 26% units increase in germination) even though the red light-stimulated germination differed with the seed batch.

scholarly journals Assessing the effects of accumulated Cd(II) on seed germination and root development of Arabidopsis thaliana

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Yejin Lee ◽  
Jinwoo Jang ◽  
Yangwon Jeon ◽  
Hyojin Kim ◽  
Geupil Jang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Inhibitory Effect ◽  
Model System ◽  
Toxic Effects ◽  
Auxin Response ◽  
E Coli ◽  
Germination And Growth ◽  
The Relationship ◽  
Visual Marker

AbstractIn this study, Arabidopsis thaliana was used as a model system to assess the toxic effects of cadmium on plant development and growth. The germination and growth of A. thaliana was inhibited by Cd(II), and the inhibitory effect was dosage-dependent. The significant decrease of germination rates and root growths of A. thaliana were observed from 50 mg/L and 25 mg/L of CdCl2, respectively. Although both shoot and root growths were suppressed by Cd(II), root developments were more sensitive to Cd(II) than shoot developments, as evidenced by shoot growths observed over 50 mg/L of CdCl2. In the concordance to this result, it was also observed that the expression of DR5::VENUS, a visual marker of auxin response, was dependent on the Cd(II) concentration and was strongly reduced from 5 mg/L of CdCl2. In addition, the E. coli-based biosensors were employed to quantify accumulated Cd(II) in plants to understand the correlation between toxic effects and Cd(II) in plants. As a result, it was revealed that 0.012 mg/g and 0.138 mg/g of Cd(II) in dried plants were corresponded to the concentration inhibiting root developments and root growths, respectively. Although it needs further investigations, the findings play a significant role in assessing the toxic effects of Cd(II) based on the relationship between the toxic effects and accumulated Cd(II) concentrations in plants.

scholarly journals Long‐term adaptation of Arabidopsis thaliana to Far‐red light

2021 ◽  
Author(s):  
Chen Hu ◽  
Wojciech J. Nawrocki ◽  
Roberta Croce
Keyword(s):  
Arabidopsis Thaliana ◽  
Red Light

The Effect of High Concentrations of Ethylene on Seed Germination of Douglas Fir (Pseudotsugamenziesii (Mirb.) Franco)

1975 ◽  
Vol 5 (3) ◽  
pp. 419-423 ◽  
Author(s):  
Carey Borno ◽  
Iain E. P. Taylor
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Douglas Fir ◽  
Control Treatment ◽  
Continuous Exposure ◽  
Short Term ◽  
Short Term Exposure ◽  
High Concentrations

Stratified, imbibed Douglas fir (Pseudotsugamenziesii (Mirb.) Franco) seeds were exposed to 100% ethylene for times between 0 and 366 h. Germination rate and germination percentage were increased by treatments up to 48 h. The 12-h treatment gave largest stimulation; 30% enhancement of final germination percentage over control. Treatment for 96 h caused increased germination rate for the first 5 days but reduced the germination percentage. Germinants were subject to continuous exposure to atmospheres containing 0.1 – 200 000 ppm ethylene in air, but it did not stimulate growth, and the gas was inhibitory above 100 ppm. Although some effects of high concentrations of ethylene may have been due to the lowering of oxygen supplies, this alone was insufficient to account for the full inhibitory effect. The mechanism of stimulation by short-term exposure to ethylene is discussed.

Allelopathic Potency and an Active Substance from Hairy Vetch

2020 ◽  
Author(s):  
Weihong Sun ◽  
Guofeng Yang ◽  
Lili Cong ◽  
Juan Sun ◽  
Lichao Ma
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Leaf Extract ◽  
Growth Inhibitor ◽  
Inhibitory Effect ◽  
Allelopathic Effect ◽  
Coumaric Acid ◽  
Hairy Vetch ◽  
Allelopathic Effects

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment,more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa.Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS).Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor.

Ontogenetic, Light, and Circadian Regulation of PAP Protein Genes during Seed Germination of Arabidopsis thaliana

2021 ◽  
Vol 500 (1) ◽  
pp. 312-316
Author(s):  
A. A. Andreeva ◽  
N. V. Kudryakova ◽  
Vl. V. Kuznetsov ◽  
V. V. Kusnetsov
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Circadian Regulation
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