scholarly journals An Expanded Survey of the Moth PBP/GOBP Clade in Bombyx mori: New Insight into Expression and Functional Roles

2021 ◽  
Vol 12 ◽  
Author(s):  
Xia Guo ◽  
Ning Xuan ◽  
Guoxia Liu ◽  
Hongyan Xie ◽  
Qinian Lou ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Expression Profile ◽  
Developmental Stages ◽  
Fat Body ◽  
Profile Analysis ◽  
The Elderly ◽  
Docking Studies ◽  
Ligand Docking ◽  
Elderly Adult ◽  
Adult Age

We studied the expression profile and ontogeny (from the egg stage through the larval stages and pupal stages, to the elderly adult age) of four OBPs from the silkworm moth Bombyx mori. We first showed that male responsiveness to female sex pheromone in the silkworm moth B. mori does not depend on age variation; whereas the expression of BmorPBP1, BmorPBP2, BmorGOBP1, and BmorGOBP2 varies with age. The expression profile analysis revealed that the studied OBPs are expressed in non-olfactory tissues at different developmental stages. In addition, we tested the effect of insecticide exposure on the expression of the four OBPs studied. Exposure to a toxic macrolide insecticide endectocide molecule (abamectin) led to the modulated expression of all four genes in different tissues. The higher expression of OBPs was detected in metabolic tissues, such as the thorax, gut, and fat body. All these data strongly suggest some alternative functions for these proteins other than olfaction. Finally, we carried out ligand docking studies and reported that PBP1 and GOBP2 have the capacity of binding vitamin K1 and multiple different vitamins.

scholarly journals Odorant Binding Protein C17 Contributes to the Response to Artemisia vulgaris Oil in Tribolium castaneum

2021 ◽  
Vol 3 ◽  
Author(s):  
Shan-Shan Gao ◽  
Rui-Min Li ◽  
Shuang Xue ◽  
Yuan-Chen Zhang ◽  
Yong-Lei Zhang ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Expression Profile ◽  
Developmental Stages ◽  
Fat Body ◽  
Profile Analysis ◽  
Control Group ◽  
Contact Toxicity ◽  
Artemisia Vulgaris ◽  
Plant Oil ◽  
Odorant Binding

The red flour beetle, Tribolium castaneum (T. castaneum), generates great financial losses to the grain storage and food processing industries. Previous studies have shown that essential oil (EO) from Artemisia vulgaris (A. vulgaris) has strong contact toxicity to larvae of the beetle, and odorant-binding proteins (OBPs) contribute to the defense of larvae against A. vulgaris. However, the functions of OBPs in insects defending against plant oil is still not clear. Here, expression of one OBP gene, TcOBPC17, was significantly induced 12–72 h after EO exposure. Furthermore, compared to the control group, RNA interference (RNAi) against TcOBPC17 resulted in a higher mortality rate after EO treatment, which suggests that TcOBPC17 involves in the defense against EO and induces a declining sensitivity to EO. In addition, the tissue expression profile analysis revealed that the expression of TcOBPC17 was more abundant in the metabolic detoxification organs of the head, fat body, epidermis, and hemolymph than in other larval tissue. The expression profile of developmental stages showed that TcOBPC17 had a higher level in early and late adult stages than in other developmental stages. Taken together, these results suggest that TcOBPC17 could participate in the sequestration process of exogenous toxicants in T. castaneum larvae.

Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis

2009 ◽  
Vol 154 (2) ◽  
pp. 188-194 ◽  
Author(s):  
Kwang Sik Lee ◽  
Bo Yeon Kim ◽  
Young Moo Choo ◽  
Hyung Joo Yoon ◽  
Pil Don Kang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Expression Profile ◽  
Cathepsin B ◽  
Fat Body

Expression profile analysis of circular RNAs in BmN cells ( Bombyx mori ) upon BmNPV infection

2020 ◽  
Vol 105 (3) ◽  
Author(s):  
Shaolun Zhang ◽  
Manman Shen ◽  
Haotong Yin ◽  
Haoling Huang ◽  
Tao Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Expression Profile ◽  
Profile Analysis ◽  
Circular Rnas ◽  
Expression Profile Analysis ◽  
Bmn Cells

scholarly journals Gene Discovery and Expression Profile Analysis through Sequencing of Expressed Sequence Tags from Different Developmental Stages of the Chytridiomycete Blastocladiella emersonii

2005 ◽  
Vol 4 (2) ◽  
pp. 455-464 ◽  
Author(s):  
Karina F. Ribichich ◽  
Silvia M. Salem-Izacc ◽  
Raphaela C. Georg ◽  
Ricardo Z. N. Vêncio ◽  
Luci D. Navarro ◽  
...  
Keyword(s):  
Life Cycle ◽  
Expression Profile ◽  
Expressed Sequence Tags ◽  
Developmental Stages ◽  
Profile Analysis ◽  
Protein Biosynthesis ◽  
Northern Analysis ◽  
Cdna Clones ◽  
Blastocladiella Emersonii ◽  
Expressed Sequence

ABSTRACT Blastocladiella emersonii is an aquatic fungus of the chytridiomycete class which diverged early from the fungal lineage and is notable for the morphogenetic processes which occur during its life cycle. Its particular taxonomic position makes this fungus an interesting system to be considered when investigating phylogenetic relationships and studying the biology of lower fungi. To contribute to the understanding of the complexity of the B. emersonii genome, we present here a survey of expressed sequence tags (ESTs) from various stages of the fungal development. Nearly 20,000 cDNA clones from 10 different libraries were partially sequenced from their 5′ end, yielding 16,984 high-quality ESTs. These ESTs were assembled into 4,873 putative transcripts, of which 48% presented no matches with existing sequences in public databases. As a result of Gene Ontology (GO) project annotation, 1,680 ESTs (35%) were classified into biological processes of the GO structure, with transcription and RNA processing, protein biosynthesis, and transport as prevalent processes. We also report full-length sequences, useful for construction of molecular phylogenies, and several ESTs that showed high similarity with known proteins, some of which were not previously described in fungi. Furthermore, we analyzed the expression profile (digital Northern analysis) of each transcript throughout the life cycle of the fungus using Bayesian statistics. The in silico approach was validated by Northern blot analysis with good agreement between the two methodologies.

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