scholarly journals Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster

2021 ◽  
Vol 12 ◽  
Author(s):  
Wojciech Krzeptowski ◽  
Lucyna Walkowicz ◽  
Ewelina Krzeptowska ◽  
Edyta Motta ◽  
Kacper Witek ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Visual System ◽  
Neurotrophic Factor ◽  
Optic Lobe ◽  
Dendritic Tree ◽  
Constant Darkness ◽  
Normal Morphology ◽  
Pigment Dispersing Factor ◽  
Group 1 ◽  
Lateral Neurons

Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) is one of a few neurotrophic factors described in Drosophila melanogaster (DmMANF) but its function is still poorly characterized. In the present study we found that DmMANF is expressed in different clusters of clock neurons. In particular, the PDF-positive large (l-LNv) and small (s-LNv) ventral lateral neurons, the CRYPTOCHROME-positive dorsal lateral neurons (LNd), the group 1 dorsal neurons posterior (DN1p) and different tim-positive cells in the fly’s visual system. Importantly, DmMANF expression in the ventral lateral neurons is not controlled by the clock nor it affects its molecular mechanism. However, silencing DmMANF expression in clock neurons affects the rhythm of locomotor activity in light:dark and constant darkness conditions. Such phenotypes correlate with abnormal morphology of the dorsal projections of the s-LNv and with reduced arborizations of the l-LNv in the medulla of the optic lobe. Additionally, we show that DmMANF is important for normal morphology of the L2 interneurons in the visual system and for the circadian rhythm in the topology of their dendritic tree. Our results indicate that DmMANF is important not only for the development of neurites but also for maintaining circadian plasticity of neurons.

scholarly journals Expression of a Drosophila melanogaster acetylcholine receptor-related gene in the central nervous system.

1988 ◽  
Vol 8 (2) ◽  
pp. 778-785 ◽  
Author(s):  
S C Wadsworth ◽  
L S Rosenthal ◽  
K L Kammermeyer ◽  
M B Potter ◽  
D J Nelson
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Drosophila Melanogaster ◽  
Acetylcholine Receptor ◽  
Optic Lobe ◽  
Cdna Probe ◽  
Amino Acid Sequence Homology ◽  
Gene Encoding ◽  
Salivary Gland Polytene Chromosome ◽  
The Central Nervous System

We isolated Drosophila melanogaster genomic sequences with nucleotide and amino acid sequence homology to subunits of vertebrate acetylcholine receptor by hybridization with a Torpedo acetylcholine receptor subunit cDNA probe. Five introns are present in the portion of the Drosophila gene encoding the unprocessed protein and are positionally conserved relative to the human acetylcholine receptor alpha-subunit gene. The Drosophila genomic clone hybridized to salivary gland polytene chromosome 3L within region 64B and was termed AChR64B. A 3-kilobase poly(A)-containing transcript complementary to the AChR64B clone was readily detectable by RNA blot hybridizations during midembryogenesis, during metamorphosis, and in newly enclosed adults. AChR64B transcripts were localized to the cellular regions of the central nervous system during embryonic, larval, pupal, and adult stages of development. During metamorphosis, a temporal relationship between the morphogenesis of the optic lobe and expression of AChR64B transcripts was observed.

scholarly journals Hypomorphic mutations in the larval photokinesis A (lphA) gene have stage-specific effects on visual system function in Drosophila melanogaster.

Genetics ◽  
1995 ◽  
Vol 139 (4) ◽  
pp. 1623-1629
Author(s):  
B Gordesky-Gold ◽  
J M Warrick ◽  
A Bixler ◽  
J E Beasley ◽  
L Tompkins
Keyword(s):  
Drosophila Melanogaster ◽  
Visual System ◽  
Specific Aspect ◽  
System Structure ◽  
P Element ◽  
System Function ◽  
Specific Effects ◽  
Isolation And Characterization ◽  
In Trans ◽  
The Many

Abstract Of the many genes that are expressed in the visual system of Drosophila melanogaster adults, some affect larval vision. However, with the exception of one X-linked mutation, no genes that have larval-specific effects on visual system structure or function have previously been reported. We describe the isolation and characterization of two mutant alleles that define the larval photokinesis A (lphA) gene, one allele of which is associated with a P-element insertion at cytogenetic locus 8E1-10. Larvae that express lphA mutations are, like normal animals, negatively photokinetic, but they are less responsive to white light than lphA + controls. Larvae that are heterozygous in trans for a mutant lphA allele and a deficiency that uncovers the lphA locus are blind, which indicates that the mutant allele is hypomorphic. lphA larvae respond normally to odorants and taste stimuli. Moreover, the lphA mutations do not affect adult flies' fast phototaxis or visually driven aspects of male sexual behavior, and electroretinograms recorded from the compound eyes of lphA/deficiency heterozygotes and lphA1/lphA2 females are normal. These observations suggest that the lphA gene affects a larval-specific aspect of visual system function.

Circadian release of pigment-dispersing factor in the visual system of the housefly,Musca domestica

2008 ◽  
Vol 509 (4) ◽  
pp. 422-435 ◽  
Author(s):  
Katarzyna Miśkiewicz ◽  
Friedrich-Wilhelm Schürmann ◽  
Elżbieta Pyza
Keyword(s):  
Visual System ◽  
Musca Domestica ◽  
Pigment Dispersing Factor

scholarly journals A neurodevelopmental origin of behavioral individuality in the Drosophila visual system

Science ◽  
2020 ◽  
Vol 367 (6482) ◽  
pp. 1112-1119 ◽  
Author(s):  
Gerit Arne Linneweber ◽  
Maheva Andriatsilavo ◽  
Suchetana Bias Dutta ◽  
Mercedes Bengochea ◽  
Liz Hellbruegge ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Visual System ◽  
Brain Development ◽  
Interindividual Variation ◽  
Visual Object ◽  
Stochastic Variation ◽  
Behavioral Variation ◽  
The Individual ◽  
And Behavior

The genome versus experience dichotomy has dominated understanding of behavioral individuality. By contrast, the role of nonheritable noise during brain development in behavioral variation is understudied. Using Drosophila melanogaster, we demonstrate a link between stochastic variation in brain wiring and behavioral individuality. A visual system circuit called the dorsal cluster neurons (DCN) shows nonheritable, interindividual variation in right/left wiring asymmetry and controls object orientation in freely walking flies. We show that DCN wiring asymmetry instructs an individual’s object responses: The greater the asymmetry, the better the individual orients toward a visual object. Silencing DCNs abolishes correlations between anatomy and behavior, whereas inducing DCN asymmetry suffices to improve object responses.

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