scholarly journals Three-Dimensional Imaging and Gene Expression Analysis Upon Enzymatic Isolation of the Tongue Epithelium

2020 ◽  
Vol 11 ◽  
Author(s):  
Christian T. Meisel ◽  
Pierfrancesco Pagella ◽  
Cristina Porcheri ◽  
Thimios A. Mitsiadis
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Enzymatic Isolation ◽  
Tongue Epithelium

From Tissues to Cell Types and Back: Single-Cell Gene Expression Analysis of Tissue Architecture

2018 ◽  
Vol 1 (1) ◽  
pp. 29-51 ◽  
Author(s):  
Xi Chen ◽  
Sarah A. Teichmann ◽  
Kerstin B. Meyer
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Three Dimensional ◽  
Cell Types ◽  
Biological Knowledge ◽  
Tissue Architecture ◽  
Cell Gene Expression ◽  
Cell Gene

With the recent transformative developments in single-cell genomics and, in particular, single-cell gene expression analysis, it is now possible to study tissues at the single-cell level, rather than having to rely on data from bulk measurements. Here we review the rapid developments in single-cell RNA sequencing (scRNA-seq) protocols that have the potential for unbiased identification and profiling of all cell types within a tissue or organism. In addition, novel approaches for spatial profiling of gene expression allow us to map individual cells and cell types back into the three-dimensional context of organs. The combination of in-depth single-cell and spatial gene expression data will reveal tissue architecture in unprecedented detail, generating a wealth of biological knowledge and a better understanding of many diseases.

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