scholarly journals Macrophage Subpopulations and the Acute Inflammatory Response of Elderly Human Skeletal Muscle to Physiological Resistance Exercise

2020 ◽  
Vol 11 ◽  
Author(s):  
Simon M. Jensen ◽  
Cecilie J. L. Bechshøft ◽  
Mette F. Heisterberg ◽  
Peter Schjerling ◽  
Jesper L. Andersen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Inflammatory Response ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Acute Inflammatory Response ◽  
Macrophage Subpopulations ◽  
Physiological Resistance

Resistance exercise increases NF-κB activity in human skeletal muscle

2012 ◽  
Vol 302 (6) ◽  
pp. R667-R673 ◽  
Author(s):  
Luke Vella ◽  
Marissa K. Caldow ◽  
Amy E. Larsen ◽  
Daniella Tassoni ◽  
Paul A. Della Gatta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dna Binding ◽  
Inflammatory Response ◽  
Resistance Exercise ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Control Group ◽  
Promoter Regions ◽  
Protein Levels ◽  
Single Bout

Intense resistance exercise causes a significant inflammatory response. NF-κB has been identified as a prospective key transcription factor mediating the postexercise inflammatory response. The purpose of this study was to determine whether a single bout of intense resistance exercise regulates NF-κB signaling in human skeletal muscle. Muscle biopsy samples were obtained from the vastus lateralis of five recreationally active, but not strength-trained, males (21.9 ± 1.3 yr) prior to, and at 2 and 4 h following, a single bout of intense resistance exercise. A further five subjects (4 males, 1 female) (23 ± 0.89 yr) were recruited as a nonexercise control group to examine the effect of the muscle biopsy protocol on key markers of skeletal muscle inflammation. Protein levels of IκBα and phosphorylated NF-κB (p65), as well as the mRNA expression of inflammatory myokines monocyte chemoattractant protein 1 (MCP-1), IL-6, and IL-8 were measured. Additionally, NF-κB (p65) DNA binding to the promoter regions of MCP-1, IL-6, and IL-8 was investigated. IκBα protein levels decreased, while p-NF-κB (p65) protein levels increased 2 h postexercise and returned to near-baseline levels by 4-h postexercise. Immunohistochemical data verified these findings, illustrating an increase in p-NF-κB (p65) protein levels, and nuclear localization at 2 h postexercise. Furthermore, NF-κB DNA binding to MCP-1, IL-6, and IL-8 promoter regions increased significantly 2 h postexercise as did mRNA levels of these myokines. No significant change was observed in the nonexercise control group. These novel data provide evidence that intense resistance exercise transiently activates NF-κB signaling in human skeletal muscle during the first few hours postexercise. These findings implicate NF-κB in the transcriptional control of myokines known to be central to the postexercise inflammatory response.

scholarly journals Arachidonic acid supplementation transiently augments the acute inflammatory response to resistance exercise in trained men

2018 ◽  
Vol 125 (2) ◽  
pp. 271-286 ◽  
Author(s):  
James F. Markworth ◽  
Randall F. D'Souza ◽  
Kirsten M. M. Aasen ◽  
Sarah M. Mitchell ◽  
Brenan R. Durainayagam ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Arachidonic Acid ◽  
Inflammatory Response ◽  
Resistance Exercise ◽  
Peripheral Blood ◽  
Inflammatory Responses ◽  
Acute Inflammatory Response ◽  
Peripheral Blood Mononuclear ◽  
Acid Supplementation ◽  
Exercise Induced

Strenuous exercise can result in skeletal muscle damage, leading to the systemic mobilization, activation, and intramuscular accumulation of blood leukocytes. Eicosanoid metabolites of arachidonic acid (ARA) are potent inflammatory mediators, but whether changes in dietary ARA intake influence exercise-induced inflammation is not known. This study investigated the effect of 4 wk of dietary supplementation with 1.5 g/day ARA ( n = 9, 24 ± 1.5 yr) or corn-soy oil placebo ( n = 10, 26 ± 1.3 yr) on systemic and intramuscular inflammatory responses to an acute bout of resistance exercise (8 sets each of leg press and extension at 80% one-repetition maximum) in previously trained men. Whole EDTA blood, serum, peripheral blood mononuclear cells (PMBCs), and skeletal muscle biopsies were collected before exercise, immediately postexercise, and at 2, 4, and 48 h of recovery. ARA supplementation resulted in higher exercise-stimulated serum creatine kinase activity [incremental area under the curve (iAUC) P = 0.046] and blood leukocyte counts (iAUC for total white cells, P < 0.001; neutrophils: P = 0.007; monocytes: P = 0.015). The exercise-induced fold change in peripheral blood mononuclear cell mRNA expression of interleukin-1β ( IL1B), CD11b ( ITGAM), and neutrophil elastase ( ELANE), as well as muscle mRNA expression of the chemokines interleukin-8 ( CXCL8) and monocyte chemoattractant protein 1 ( CCL2) was also greater in the ARA group than placebo. Despite this, ARA supplementation did not influence the histological presence of leukocytes within muscle, perceived muscle soreness, or the extent and duration of muscle force loss. These data show that ARA supplementation transiently increased the inflammatory response to acute resistance exercise but did not impair recovery.NEW & NOTEWORTHY Daily arachidonic acid supplementation for 4 wk in trained men augmented the acute systemic and intramuscular inflammatory response to a subsequent bout of resistance exercise. Greater exercise-induced inflammatory responses in men receiving arachidonic acid supplementation were not accompanied by increased symptoms of exercise-induced muscle damage. Although increased dietary arachidonic acid intake does not appear to influence basal inflammation in humans, the acute inflammatory response to exercise stress is transiently increased following arachidonic acid supplementation.

scholarly journals Resistance Exercise Reverses Aging in Human Skeletal Muscle

PLoS ONE ◽  
2007 ◽  
Vol 2 (5) ◽  
pp. e465 ◽  
Author(s):  
Simon Melov ◽  
Mark A. Tarnopolsky ◽  
Kenneth Beckman ◽  
Krysta Felkey ◽  
Alan Hubbard
Keyword(s):  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle

scholarly journals Heat stress enhances mTOR signaling after resistance exercise in human skeletal muscle

2011 ◽  
Vol 61 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Ryo Kakigi ◽  
Hisashi Naito ◽  
Yuji Ogura ◽  
Hiroyuki Kobayashi ◽  
Norio Saga ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Heat Stress ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Mtor Signaling

Transcriptional Signatures of Human Skeletal Muscle in Response to Aerobic and Resistance Exercise

2017 ◽  
Vol 49 (5S) ◽  
pp. 339
Author(s):  
Jared M. Dickinson ◽  
Andrew C. D’Lugos ◽  
Marcus Naymik ◽  
Matt De Both ◽  
Ashley Siniard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle

scholarly journals Aerobic and resistance exercise training reverses age‐dependent decline in NAD + salvage capacity in human skeletal muscle

2019 ◽  
Vol 7 (12) ◽  
Author(s):  
Roldan M. de Guia ◽  
Marianne Agerholm ◽  
Thomas S. Nielsen ◽  
Leslie A. Consitt ◽  
Ditte Søgaard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Exercise Training ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Resistance Exercise Training ◽  
Age Dependent

The Impact of Acetaminophen Consumption on mTOR Signaling in Human Skeletal Muscle Following Resistance Exercise

2017 ◽  
Vol 49 (5S) ◽  
pp. 801
Author(s):  
Andrew C. D’Lugos ◽  
Shivam H. Patel ◽  
Jordan C. Ormsby ◽  
Tara N. Mahmood ◽  
Don P. Curtis ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Mtor Signaling ◽  
The Impact

scholarly journals Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle

Autophagy ◽  
2015 ◽  
Vol 11 (3) ◽  
pp. 538-546 ◽  
Author(s):  
Anna Ulbricht ◽  
Sebastian Gehlert ◽  
Barbara Leciejewski ◽  
Thorsten Schiffer ◽  
Wilhelm Bloch ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Selective Autophagy
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