scholarly journals Aquaporin-7: A Dynamic Aquaglyceroporin With Greater Water and Glycerol Permeability Than Its Bacterial Homolog GlpF

2020 ◽  
Vol 11 ◽  
Author(s):  
Fraser J. Moss ◽  
Paween Mahinthichaichan ◽  
David T. Lodowski ◽  
Thomas Kowatz ◽  
Emad Tajkhorshid ◽  
...  
Keyword(s):  
Aquaporin 7 ◽  
Bacterial Homolog ◽  
Glycerol Permeability

Aquaporin-7 and glycerol permeability as novel obesity drug-target pathways

2006 ◽  
Vol 27 (7) ◽  
pp. 345-347 ◽  
Author(s):  
G FRUHBECK ◽  
V CATALAN ◽  
J GOMEZAMBROSI ◽  
A RODRIGUEZ
Keyword(s):  
Drug Target ◽  
Aquaporin 7 ◽  
Glycerol Permeability

scholarly journals Progressive Adipocyte Hypertrophy in Aquaporin-7-deficient Mice

2005 ◽  
Vol 280 (16) ◽  
pp. 15493-15496 ◽  
Author(s):  
Mariko Hara-Chikuma ◽  
Eisei Sohara ◽  
Tatemitsu Rai ◽  
Masahito Ikawa ◽  
Masaru Okabe ◽  
...  
Keyword(s):  
Body Fat ◽  
Fat Mass ◽  
Body Fat Mass ◽  
Wild Type ◽  
Adipocyte Volume ◽  
Aquaporin 7 ◽  
Adipocyte Hypertrophy ◽  
Adipocyte Plasma Membranes ◽  
Deficient Mice ◽  
Glycerol Permeability

Aquaporin-7 (AQP7) is a water/glycerol transporting protein expressed in adipocyte plasma membranes. We report here remarkable age-dependent hypertrophy in adipocytes in AQP7-deficient mice. Wild type and AQP7 null mice had similar growth at 0–16 weeks as assessed by body weight; however, by 16 weeks AQP7 null mice had 3.7-fold increased body fat mass. Adipocytes from AQP7 null mice of age 16 weeks were greatly enlarged (diameter 118 μm) compared with wild type mice (39 μm). Adipocytes from AQP7 null mice also accumulated excess glycerol (251versus86 nmol/mg of protein) and triglycerides (3.4versus1.7 μmol/mg of protein). In contrast, at age 4 weeks, adipocyte volume and body fat mass were comparable in wild type and AQP7 null mice. To investigate the mechanism(s) responsible for the progressive adipocyte hypertrophy, glycerol permeability and fat metabolism were studied in adipocytes isolated from the younger mice. Plasma membrane glycerol permeability measured by [14C]glycerol uptake was 3-fold reduced in AQP7-deficient adipocytes. However, adipocyte lipolysis, measured by free fatty acid release and hormone-sensitive lipase activity, and lipogenesis, measured by [14C]glucose incorporation into triglycerides, were not affected by AQP7 deletion. These data suggest that adipocyte hypertrophy in AQP7 deficiency results from defective glycerol exit and consequent accumulation of glycerol and triglycerides. Increasing AQP7 expression/function in adipocytes may reduce adipocyte volume and fat mass in obesity.

scholarly journals Insights into the Human Glutamate Transporters from the Bacterial Homolog Glt(Ph)

2013 ◽  
Vol 104 (2) ◽  
pp. 109a
Author(s):  
Germano Heinzelmann ◽  
Serdar Kuyucak
Keyword(s):  
Glutamate Transporters ◽  
Bacterial Homolog

scholarly journals Analysis of Htra Gene from Zebrafish (Danio Rerio)

2015 ◽  
Vol 10 (2) ◽  
Author(s):  
M. Murwantoko ◽  
Chio Oka ◽  
Masashi Kawaichi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Danio Rerio ◽  
Serine Proteases ◽  
Catalytic Domain ◽  
Fruit Fly ◽  
Wide Range ◽  
Igf Binding ◽  
Bacterial Homolog

HtrA which is characterized by the combination of a trypsin-like catalytic domain with at least one C-terminalPDZ domain is a highly conserved family of serine proteases found in a wide range of organisms. However theidentified HtrA family numbers varies among spesies, for example the number of mammalian, Eschericia coli,fruit fly-HtrA family are 4, 3 and 1 gene respectively. One gene is predicted exist in zebrafish. Since no completeinformation available on zebrafish HtrA, in this paper zebrafish HtrA (zHtrA) gene was analyzed. The zHtrA isbelonged to HtrA1 member and predicted encodes 478 amino acids with a signal peptide, a IGF binding domain,a Kazal-type inhibitor domain in the up stream of HtrA-bacterial homolog. At the amino acid sequence the zHtrA1showed the 69%, 69%, 68%, 54% and 54% with the rat HtrA1, mouse HtrA1, human HtrA1, human HtrA3 andmouse HtrA4 respectively. The zHtrA1 is firstly expressed at 60 hpf and mainly in the vertebral rudiments in thetail region.

scholarly journals Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245739
Author(s):  
Inês Vieira da Silva ◽  
Bárbara P. Soares ◽  
Catarina Pimpão ◽  
Rui M. A. Pinto ◽  
Teresa Costa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Biochemical Parameters ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Mrna Levels ◽  
Promising Target ◽  
Dietary Treatments ◽  
Glycerol Permeability

The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation.

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