scholarly journals Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum

2020 ◽  
Vol 11 ◽  
Author(s):  
Zibo Li ◽  
Yaoyao Zhang ◽  
Xingkui An ◽  
Qi Wang ◽  
Adel Khashaveh ◽  
...  
Keyword(s):  
Apolygus Lucorum ◽  
Chemosensory Genes

Chemosensory genes in the head of Spodoptera litura larvae

2021 ◽  
pp. 1-10
Author(s):  
Lu-Lu Li ◽  
Ji-Wei Xu ◽  
Wei-Chen Yao ◽  
Hui-Hui Yang ◽  
Youssef Dewer ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Phylogenetic Trees ◽  
Target Genes ◽  
Control Strategies ◽  
Insect Pest ◽  
Management Strategies ◽  
Food Sources ◽  
Larval Feeding ◽  
Chemosensory System ◽  
Chemosensory Genes

Abstract The tobacco cutworm Spodoptera litura (Lepidoptera: Noctuidae) is a polyphagous pest with a highly selective and sensitive chemosensory system involved in complex physiological behaviors such as searching for food sources, feeding, courtship, and oviposition. However, effective management strategies for controlling the insect pest populations under threshold levels are lacking. Therefore, there is an urgent need to formulate eco-friendly pest control strategies based on the disruption of the insect chemosensory system. In this study, we identified 158 putative chemosensory genes based on transcriptomic and genomic data for S. litura, including 45 odorant-binding proteins (OBPs, nine were new), 23 chemosensory proteins (CSPs), 60 odorant receptors (ORs, three were new), and 30 gustatory receptors (GRs, three were new), a number higher than those reported by previous transcriptome studies. Subsequently, we constructed phylogenetic trees based on these genes in moths and analyzed the dynamic expression of various genes in head capsules across larval instars using quantitative real-time polymerase chain reaction. Nine genes–SlitOBP8, SlitOBP9, SlitOBP25, SlitCSP1, SlitCSP7, SlitCSP18, SlitOR34, SlitGR240, and SlitGR242–were highly expressed in the heads of 3- to 5-day-old S. litura larvae. The genes differentially expressed in olfactory organs during larval development might play crucial roles in the chemosensory system of S. litura larvae. Our findings substantially expand the gene inventory for S. litura and present potential target genes for further studies on larval feeding in S. litura.

Fructose and glucose in buckwheat nectar enhance Peristenus spretus (Hymenoptera: Braconidae) survival and parasitism of the mirid Apolygus lucorum

2021 ◽  
pp. 104710
Author(s):  
Shike Xia ◽  
Shuping Luo ◽  
Jiaqun Li ◽  
Qing Yang ◽  
Changchun Dai ◽  
...  
Keyword(s):  
Apolygus Lucorum

scholarly journals Temperature-dependent life history of the green plant bug, Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae)

2010 ◽  
Vol 45 (3) ◽  
pp. 387-393 ◽  
Author(s):  
Yanhui Lu ◽  
Kongming Wu ◽  
Kris A. G. Wyckhuys ◽  
Yuyuan Guo
Keyword(s):  
Life History ◽  
Green Plant ◽  
Temperature Dependent ◽  
Apolygus Lucorum ◽  
History Of

scholarly journals Candidate Chemosensory Genes in the Stemborer Sesamia nonagrioides

2013 ◽  
Vol 9 (5) ◽  
pp. 481-495 ◽  
Author(s):  
Nicolas Glaser ◽  
Aurore Gallot ◽  
Fabrice Legeai ◽  
Nicolas Montagné ◽  
Erwan Poivet ◽  
...  
Keyword(s):  
Sesamia Nonagrioides ◽  
Chemosensory Genes

Identification and analysis of chemosensory genes encoding odorant-binding proteins, chemosensory proteins and sensory neuron membrane proteins in the antennae of Lissorhoptrus oryzophilus

2021 ◽  
pp. 1-11
Author(s):  
Yu Pan ◽  
Xinxin Zhang ◽  
Zhun Wang ◽  
Lizhong Qi ◽  
Xinsheng Zhang ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sensory Neuron ◽  
Binding Proteins ◽  
Mate Location ◽  
Lissorhoptrus Oryzophilus ◽  
Neuron Membrane ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Chemosensory Genes ◽  
Odorant Binding

Abstract The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive pest that causes damage to rice crops worldwide. The olfactory system is critical for host or mate location by weevils, but only limited information about the molecular mechanism of olfaction-related behaviour has been reported in this insect. In this study, we conducted SMRT-seq transcriptome analysis and obtained 54,378 transcripts, 38,706 of which were annotated. Based on these annotations, we identified 40 candidate chemosensory genes, including 31 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs) and three sensory neuron membrane proteins (SNMPs). Phylogenetic analysis showed that LoryOBPs, LoryCSPs and LorySNMPs were distributed in various clades. The results of tissue expression patterns indicated that LoryOBPs were highly abundant in the antennae, whereas LoryCSPs were highly abundant not only in the antennae but also in the abdomen, head and wings. Our findings substantially expand the gene database of L. oryzophilus and may serve as a basis for identifying novel targets to disrupt key olfactory genes, potentially providing an eco-friendly strategy to control this pest in the future.

Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals

2014 ◽  
Vol 60 ◽  
pp. 31-39 ◽  
Author(s):  
Yan-Le Zhou ◽  
Xiao-Qiang Zhu ◽  
Shao-Hua Gu ◽  
Huan-huan Cui ◽  
Yu-Yuan Guo ◽  
...  
Keyword(s):  
Rna Interference ◽  
Apolygus Lucorum

scholarly journals Analysis of Differentially Expressed Transcripts in Apolygus lucorum (Meyer-Dür) Exposed to Different Temperature Coefficient Insecticides

2020 ◽  
Vol 21 (2) ◽  
pp. 658 ◽  
Author(s):  
Jingjie An ◽  
Chang Liu ◽  
Ya’nan Dou ◽  
Zhanlin Gao ◽  
Zhihong Dang ◽  
...  
Keyword(s):  
Temperature Effect ◽  
Temperature Coefficient ◽  
Transcriptome Profiling ◽  
Underlying Mechanism ◽  
Green Plant ◽  
Rna Seq ◽  
Biochemical Processes ◽  
Apolygus Lucorum ◽  
Gene Information ◽  
Physiological And Biochemical

The existence of a temperature effect of insecticides frustrated the control of the green plant bug Apolygus lucorum (Meyer-Dür). Previous studies mostly focused on the application of insecticides, but the underlying mechanism remains incompletely understood. Here, we report a transcriptome profiling of A. lucorum treated by three kinds of temperature coefficient insecticides (TCIs) (positive TCI: imidacloprid, negative TCI: b-cypermethrin and non-effect TCI: phoxim) at 15 °C, 25 °C and 35 °C by using next- and third-generation RNA-Seq methods. A total of 34,739 transcripts were annotated from 277.74 Gb of clean data. There were more up-regulated transcripts than down-regulated transcripts in all three kinds of TCI treatments. Further Venn diagrams indicate the regulatory transcripts and regulatory modes were different at the three temperatures. The responses to imidacloprid involved more detox and stress response transcripts such as cytochrome P450 (CYP450), carboxylesterase (CarE) and catalase (CAT) at 35 °C, which was the case for beta-cypermethrin at 15 °C. UDP-glucuronyltransferase (UGT) and heat shock protein (HSP) transcripts were heavily involved, and thus deserve particular note in the temperature effect of insecticides. This high-confidence transcriptome atlas provides improved gene information for further study on the insecticide temperature effect related physiological and biochemical processes of A. lucorum.

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