scholarly journals Dilution of Seawater Affects the Ca2 + Transport in the Outer Mantle Epithelium of Crassostrea gigas

2020 ◽  
Vol 11 ◽  
Author(s):  
J. Kirsikka Sillanpää ◽  
Joao Carlos dos Reis Cardoso ◽  
Rute Castelo Félix ◽  
Liliana Anjos ◽  
Deborah Mary Power ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Outer Mantle Epithelium

scholarly journals Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas

2018 ◽  
Vol 285 (1891) ◽  
pp. 20181676 ◽  
Author(s):  
J. Kirsikka Sillanpää ◽  
Henrik Sundh ◽  
Kristina S. Sundell
Keyword(s):  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
Ussing Chamber ◽  
Shell Growth ◽  
Kinetic Studies ◽  
Linear Component ◽  
Growth Area ◽  
The Pacific ◽  
Marine Bivalves ◽  
Outer Mantle Epithelium

Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca 2+ ) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca 2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca 2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a K m of 6.2 mM and a V max of 3.3 nM min −1 . The transcellular transfer of Ca 2+ accounts for approximately 60% of the total Ca 2+ transfer across the OME of C. giga s at environmental Ca 2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca 2+ -channels, plasma-membrane Ca 2+ -ATPase and Na + /Ca 2+ -exchanger all participate in the transcellular Ca 2+ transfer across the OME and a model for this Ca 2+ transfer is presented and discussed.

Retos de la acuicultura marina litoral: Caso cultivo de ostras en el Golfo de Nicoya, Costa Rica

2019 ◽  
Author(s):  
Rebeca Quesada Céspedes ◽  
Sidey Arias Valverde ◽  
Oscar Pacheco Urpí ◽  
Gerardo Zúñiga Calero ◽  
Oscar José Pacheco Prieto ◽  
...  
Keyword(s):  
Costa Rica ◽  
Crassostrea Gigas

Costa Rica muestra una condición incipiente en el desarrollo de la acuicultura marina, a pesar de los esfuerzos invertidos por más de veinte años. La experiencia en el cultivo de Crassostrea gigas alcanzada por comunidades, la institucionalidad pública y ONG´s, desde el ámbito social- económico y ambiental, evidencian que el país cuenta con las condiciones idóneas, para direccionar la ostricultura como un nuevo sector socio-económico con y desde las comunidades marino costeras del litoral pacífico. El presente trabajo expone los logros obtenidos por comunidades y actores locales; además los retos que ambas partes se han propuesto para hacer de esta actividad una herramienta más que contribuye con la sustentabilidad y empoderamiento de familias y comunidades que actualmente se encuentran en condiciones de pobreza.

Баркодинг митохондриальной ДНК тихоокеанской устрицы Crassostrea gigas (Thunberg, 1793) (Mollusca: Bivalvia: Ostreidae), культивируемой в Черном море

2018 ◽  
Vol 54 (12) ◽  
pp. 1419-1425
Author(s):  
Ю. В. Слынько ◽  
Е. Е. Слынько ◽  
А. В. Пиркова ◽  
Л. В. Ладыгина ◽  
В. И. Рябушко
Keyword(s):  
Crassostrea Gigas

A novel heptapeptide derived from Crassostrea gigas shows anticoagulant activity by targeting for thrombin active domain

2021 ◽  
Vol 334 ◽  
pp. 127507
Author(s):  
Shuzhen Cheng ◽  
Maolin Tu ◽  
Hanxiong Liu ◽  
Yue An ◽  
Ming Du ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Anticoagulant Activity

scholarly journals Meeting the Salinity Requirements of the Bivalve Mollusc Crassostrea gigas in the Depuration Process and Posterior Shelf-Life Period to Improve Food Safety and Product Quality

Water ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1126
Author(s):  
João A. Silvestre ◽  
Sílvia F. S. Pires ◽  
Vitória Pereira ◽  
Miguel Colaço ◽  
Ana P. L. Costa ◽  
...  
Keyword(s):  
Food Safety ◽  
Shelf Life ◽  
Crassostrea Gigas ◽  
Storage Period ◽  
Microbiological Contamination ◽  
Bivalve Molluscs ◽  
Antioxidant Defences ◽  
Life Period ◽  
Microbiological Analyses ◽  
Depuration Process

Microbiological contamination of bivalve molluscs is one of the major concerns inherent to food safety, thus depuration is frequently needed to assure food safety levels associated with their consumption. Salinity plays an important role in the metabolic activity of bivalves and as such can influence their depuration capacity. This study aimed to evaluate the effect of salinity (25, 30, 35 and 40) on the efficiency of the depuration process, along with the quality and shelf-life of Crassostrea gigas. For this, a 24-h depuration was carried out, followed by a storage period at 5 ± 1 °C for six days. Microbiological analyses and biochemical parameters related to oxidative stress response were analysed. Escherichia coli load was reduced in only 24 h, disregarding the salinity of the system. After the shelf-life period, the activity of the antioxidant defences at salinities 35 and 40 is higher but is still not sufficient to avoid lipid peroxidation. Over time, there is a decrease in oyster metabolism probably due to being chilled and to the action of exposure to air. In sum, this study suggests salinities between 25 and 30 as preferential for the depuration process of C. gigas and subsequent quality during shelf-life.

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