scholarly journals Editorial: Insect Olfactory Proteins (From Gene Identification to Functional Characterization)

2019 ◽  
Vol 10 ◽  
Author(s):  
Peng He ◽  
Nicolas Durand ◽  
Shuang-Lin Dong
Keyword(s):  
Functional Characterization ◽  
Gene Identification ◽  
Olfactory Proteins

scholarly journals Functional characterization of the common c.-32-13T>G mutation of GAA gene: identification of potential therapeutic agents

2013 ◽  
Vol 42 (2) ◽  
pp. 1291-1302 ◽  
Author(s):  
A. Dardis ◽  
I. Zanin ◽  
S. Zampieri ◽  
C. Stuani ◽  
A. Pianta ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Therapeutic Agents ◽  
Gene Identification ◽  
The Common ◽  
C 32 ◽  
Gaa Gene

Target gene identification and functional characterization of miR-1704 in chicken

2019 ◽  
Vol 31 (3) ◽  
pp. 229-236
Author(s):  
Yongcai Wang ◽  
Shunhong Wang ◽  
Shanghe Wang ◽  
Guoxi Li ◽  
RuiRui Jiang ◽  
...  
Keyword(s):  
Target Gene ◽  
Functional Characterization ◽  
Gene Identification

A Novel Reconstitution Method for Inducing the Formation of Regular 2-Dimensional Arrays of Membrane Proteins and Lipids

1988 ◽  
Vol 46 ◽  
pp. 152-153 ◽  
Author(s):  
A. Engel ◽  
A. Holzenburg ◽  
K. Stauffer ◽  
J. Rosenbusch ◽  
U. Aebi
Keyword(s):  
Membrane Proteins ◽  
Flow Rate ◽  
Lipid Membranes ◽  
Functional Characterization ◽  
Dimensional Structure ◽  
Electron Crystallography ◽  
Peltier Element ◽  
Protein Ratio ◽  
Precise Control ◽  
3 Dimensional

Reconstitution of solubilized and purified membrane proteins in the presence of phospholipids into vesicles allows their functions to be studied by simple bulk measurements (e.g. diffusion of differently sized solutes) or by conductance measurements after transformation into planar membranes. On the other hand, reconstitution into regular protein-lipid arrays, usually forming at a specific lipid-to-protein ratio, provides the basis for determining the 3-dimensional structure of membrane proteins employing the tools of electron crystallography.To refine reconstitution conditions for reproducibly inducing formation of large and highly ordered protein-lipid membranes that are suitable for both electron crystallography and patch clamping experiments aimed at their functional characterization, we built a flow-dialysis device that allows precise control of temperature and flow-rate (Fig. 1). The flow rate is generated by a peristaltic pump and can be adjusted from 1 to 500 ml/h. The dialysis buffer is brought to a preselected temperature during its travel through a meandering path before it enters the dialysis reservoir. A Z-80 based computer controls a Peltier element allowing the temperature profile to be programmed as function of time.

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