scholarly journals Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert Living Lepus yarkandensis

2019 ◽  
Vol 10 ◽  
Author(s):  
Jianping Zhang ◽  
Shuwei Li ◽  
Fang Deng ◽  
Buheliqihan Baikeli ◽  
Shuguang Huang ◽  
...  
Keyword(s):  
Expression Levels ◽  
Arid Desert ◽  
Aquaporin Family

Higher expression levels of aquaporin (AQP)1 and AQP5 in the lungs of arid‐desert living Lepus yarkandensis

2019 ◽  
Vol 104 (4) ◽  
pp. 1186-1195
Author(s):  
Jianping Zhang ◽  
Shuwei Li ◽  
Jie Liu ◽  
Lexing Li ◽  
Fang Deng ◽  
...  
Keyword(s):  
Expression Levels ◽  
Arid Desert

Gene expression analysis of metabolic markers during bone regeneration in a rat model

2014 ◽  
Vol 23 (03) ◽  
pp. 207-211
Author(s):  
C. Kasch ◽  
A. Osterberg ◽  
Thordis Granitzka ◽  
T. Lindner ◽  
M. Haenle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Saline Solution ◽  
Female Rats ◽  
Synthesis Rate ◽  
Metabolic Markers ◽  
Expression Levels ◽  
Fibrotic Tissue ◽  
Intermittent Pth ◽  
Lower Expression

SummaryThe RANK/RANKL/OPG system plays an important role in the regulation of bone metabolism and bony integration around implants. The aim of this study was to analyse gene expression of OPG, RANK, and RANKL in regenerating bone during implant integration. Additionally, the effect of intermittent para - thyroid hormone (PTH) treatment was analysed. A titanium chamber was implanted in the proximal tibiae of 48 female rats. The animals received either human PTH or saline solution (NaCl). After 21 and 42 days, RNA was isolated from tissue adjacent to the implant and expression of RANK, RANKL, and OPG was analysed. After 21 days, very low expression levels of all genes were shown. In contrast, increased gene expression after 42 days was determined. Expression of RANK and RANKL was lower than that for OPG. The lower expression levels after 21 days might be due to still ossifying, fibrotic tissue around the titanium chamber. An increased OPG synthesis rate associated with decreased RANKL expression after 42 days revealed bone-forming processes. Despite significant differences in gene expression between the time points, only slight differences were observed between application of intermittent PTH and NaCl after a period of 42 days.

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽  
2018 ◽  
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Rungnapa Leelatanawit ◽  
Somjai Wongtriphop ◽  
Jutatip Khudet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Chain Fatty Acid ◽  
Regulatory Genes ◽  
Ovarian Maturation ◽  
Long Chain Fatty Acid ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

Measuring MicroRNA Expression Levels in Oncology: from Samples to Data Analysis

2013 ◽  
Vol 18 (4) ◽  
pp. 273-287 ◽  
Author(s):  
Loris De Cecco ◽  
Matteo Dugo ◽  
Silvana Canevari ◽  
Maria Grazia Daidone ◽  
Maurizio Callari
Keyword(s):  
Data Analysis ◽  
Microrna Expression ◽  
Expression Levels

Climate Change and Drought: Impact of Food Insecurity on Gender Based Vulnerability in District Tharparkar

2018 ◽  
Vol 57 (3) ◽  
pp. 307-321 ◽  
Author(s):  
Manzoor Hussain Memon ◽  
Naveed Aamir ◽  
Nadeem Ahmed
Keyword(s):  
Climate Change ◽  
Food Insecurity ◽  
Water Scarcity ◽  
Work Load ◽  
Jel Classification ◽  
Arid Ecosystems ◽  
Global Changes ◽  
The People ◽  
Gender Based ◽  
Arid Desert

Climate change has now become a reality that has intensified the sufferings of people living in arid ecosystems. Decrease in rainfall, rise in temperature and increase in the frequency of extreme events are some of the changes observed in the semi-arid desert of district Tharparkar. For thousands of years, people of Tharparkar are coping with drought and aridity of the land by using indigenous knowledge. However, global changes in the climatic pattern and deterioration of social and economic conditions have pushed the inhabitants of this arid region into extreme vulnerable situation. This paper investigates the link between climate-induced natural disasters, particularly drought, from the perspective of changing climate patterns which have resulted in food insecurity and water scarcity. The paper analyses the rainfall pattern in the last 38 years—dividing it into two periods i.e. from 1975-1994 and 1995-2014. The findings of the paper have challenged the prevailing notions about aridity and rainfall patterns in Tharparkar district. The research found that there is an increase in average annual precipitation in the district with erratic patterns. Thus, the nature of drought in the district has changed from its historic pattern of less or no rainfall to more but erratic rainfall that is more threatening to livelihoods of the people that in turn have multiplier effect on water and food insecurity. In particularly, women are more vulnerable in the absence of social security and lack of basic necessities for their survival amidst drought. For instance, traditionally the burden of managing water resources falls on women, which leads to an increased work load during the time of drought and also water scarcity. JEL Classification: Q54, Q56, Q25, I30 Keywords: Climate, Environment and Development, Drought, Water, Poverty

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