scholarly journals Dynamin Binding Protein Is Required for Xenopus laevis Kidney Development

2019 ◽  
Vol 10 ◽  
Author(s):  
Bridget D. DeLay ◽  
Tanya A. Baldwin ◽  
Rachel K. Miller
Keyword(s):  
Xenopus Laevis ◽  
Kidney Development ◽  
Binding Protein

scholarly journals Dynamin binding protein is required for Xenopus laevis kidney development

2018 ◽  
Author(s):  
Bridget D. DeLay ◽  
Tanya A. Baldwin ◽  
Rachel K. Miller
Keyword(s):  
Xenopus Laevis ◽  
Antisense Oligonucleotides ◽  
Kidney Development ◽  
Binding Protein ◽  
Human Kidney ◽  
Normal Kidney ◽  
Similar Reduction ◽  
Developmental Defects ◽  
Early Markers ◽  
Developing Kidney

ABSTRACTThe adult human kidney contains over one million nephrons, with each nephron consisting of a tube containing segments that have specialized functions in nutrient and water absorption and waste excretion. The embryonic kidney of Xenopus laevis consists of a single functional nephron composed of regions that are analogous to those found in the human nephron, making it a simple model for the study of nephrogenesis. The exocyst complex, which traffics proteins to the cell membrane in vesicles via CDC42, is essential for normal kidney development. Here, we show that the CDC42-GEF, dynamin binding protein (Dnmbp/Tuba), is essential for nephrogenesis in Xenopus. dnmbp is expressed in Xenopus embryo kidneys during development, and knockdown of Dnmbp using two separate morpholino antisense oligonucleotides results in reduced expression of late pronephric markers, whereas the expression of early markers of nephrogenesis remains unchanged. A greater reduction in expression of markers of differentiated distal and connecting tubules was seen in comparison to proximal tubule markers, indicating that Dnmbp reduction may have a greater impact on distal and connecting tubule differentiation. dnmbp knockout using CRISPR results in a similar reduction of late markers of pronephric tubulogenesis. Overexpression of dnmbp in the kidney also resulted in disrupted pronephric tubules, suggesting that dnmbp levels in the developing kidney are tightly regulated, with either increased or decreased levels leading to developmental defects. Together, these data suggest that Dnmbp is required for nephrogenesis.

A cDNA clone for a polyadenylated RNA-binding protein of Xenopus laevis oocytes hybridizes to four developmentally regulated mRNAs

1985 ◽  
Vol 5 (10) ◽  
pp. 2697-2704
Author(s):  
L J Lorenz ◽  
J D Richter
Keyword(s):  
Xenopus Laevis ◽  
Cdna Clone ◽  
Rna Binding ◽  
Binding Protein ◽  
Single Gene ◽  
In Vitro Translation ◽  
Xenopus Laevis Oocytes ◽  
Cdna Insert ◽  
Alternative Processing

Xenopus laevis oocytes contain a unique group of proteins which decrease during oogenesis, bind poly(A) RNA, and possibly play a role in the regulation of translation. A monoclonal antibody generated against one of these proteins was used to screen an expression vector cDNA library. A cDNA clone was isolated and confirmed to code for the binding protein by in vitro translation of hybrid-selected RNA followed by immunoprecipitation. This cDNA, when used in RNA gel blots, hybridized to four transcripts of 2.0, 1.7 (two transcripts of similar size), and 1.2 kilobases. All of the transcripts decreased in amount during oogenesis and were not evident in somatic cells. In addition, the fraction of the transcripts associated with polysomes decreased during oogenesis. Digestion of the cDNA insert with PstI generated two fragments of 220 and 480 base pairs which, when used as probes in an RNA gel blot, hybridized to unique as well as common transcripts. Genomic Southern blots suggested the presence of a single gene, indicating that these transcripts arose by alternative processing.

scholarly journals The RNA-binding protein Xp54nrb isolated from a Ca2+-dependent screen is expressed in neural structures during Xenopus laevis development

2011 ◽  
Vol 55 (10-11-12) ◽  
pp. 923-931 ◽  
Author(s):  
Isabelle Neant ◽  
Nina Deisig ◽  
Pierluigi Scerbo ◽  
Catherine Leclerc ◽  
Marc Moreau
Keyword(s):  
Xenopus Laevis ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

cDNA cloning and developmental expression of cellular nucleic acid-binding protein (CNBP) gene in Xenopus laevis

Gene ◽  
2000 ◽  
Vol 241 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Alessandra De Dominicis ◽  
Francesco Lotti ◽  
Paola Pierandrei-Amaldi ◽  
Beatrice Cardinali
Keyword(s):  
Nucleic Acid ◽  
Xenopus Laevis ◽  
Cdna Cloning ◽  
Binding Protein ◽  
Developmental Expression ◽  
Nucleic Acid Binding ◽  
Nucleic Acid Binding Protein ◽  
Acid Binding Protein

scholarly journals Embryonic Poly(A)-Binding Protein Stimulates Translation in Germ Cells

2005 ◽  
Vol 25 (5) ◽  
pp. 2060-2071 ◽  
Author(s):  
Gavin S. Wilkie ◽  
Philippe Gautier ◽  
Diane Lawson ◽  
Nicola K. Gray
Keyword(s):  
Xenopus Laevis ◽  
Oocyte Maturation ◽  
Germ Cells ◽  
Binding Protein ◽  
Expression Patterns ◽  
General Role ◽  
Early Embryos ◽  
Relative Divergence

ABSTRACT The function of poly(A)-binding protein 1 (PABP1) in poly(A)-mediated translation has been extensively characterized. Recently, Xenopus laevis oocytes and early embryos were shown to contain a novel poly(A)-binding protein, ePABP, which has not been described in other organisms. ePABP was identified as a protein that binds AU-rich sequences and prevents shortening of poly(A) tails. Here, we show that ePABP is also expressed in X. laevis testis, suggesting a more general role for ePABP in gametogenesis. We find that ePABP is conserved throughout vertebrates and that mouse and X. laevis cells have similar tissue-specific ePABP expression patterns. Furthermore, we directly assess the role of ePABP in translation. We show that ePABP is associated with polysomes and can activate the translation of reporter mRNAs in vivo. Despite its relative divergence from PABP1, we find that ePABP has similar functional domains and can bind to several PABP1 partners, suggesting that they may use similar mechanisms to activate translation. In addition, we find that PABP1 and ePABP can interact, suggesting that these proteins may be bound simultaneously to the same mRNA. Finally, we show that the activity of both PABP1 and ePABP increases during oocyte maturation, when many mRNAs undergo polyadenylation.

scholarly journals Expression of the Ca2+-binding protein, parvalbumin, during embryonic development of the frog, Xenopus laevis

1987 ◽  
Vol 104 (4) ◽  
pp. 841-847 ◽  
Author(s):  
BK Kay ◽  
AJ Shah ◽  
WE Halstead
Keyword(s):  
Xenopus Laevis ◽  
Fusion Protein ◽  
Binding Protein ◽  
Expression Library ◽  
Simple Method ◽  
High Affinity ◽  
Tadpole Tail ◽  
Developing Muscle ◽  
Beta Galactosidase ◽  
Xenopus Laevis Tadpole

A cDNA segment encoding the Ca2+-binding protein, parvalbumin, was isolated with the use of antibodies, from a lambda gtll expression library of Xenopus laevis tadpole poly(A)+ RNAs. The bacterially expressed beta-galactosidase-parvalbumin fusion protein of one lambda recombinant shows high affinity 45Ca2+ binding. The sequence of the tadpole parvalbumin is highly similar to previously characterized beta-parvalbumins of other organisms. Data from protein and RNA blotting experiments demonstrate that parvalbumin is absent in oocytes, eggs, and early staged embryos, and only becomes expressed during embryogenesis at the time of myogenesis. The protein can be detected in individual developing muscle cells and in muscle fibers of tadpole tail muscles. A simple method is also described for the isolation of neural tube-notochord-somite complexes from Xenopus embryos.

A Serpin with MT 43,000 Is a Binding Protein of Mr 25,000 Protein, a Substrate for Protein Ser/Thr Kinase Detected in Xenopus laevis Oocytes

2001 ◽  
Vol 129 (2) ◽  
pp. 229-236 ◽  
Author(s):  
N. Goto ◽  
S. Muto ◽  
I. Sugimoto ◽  
K. Ikawa-Kitayama ◽  
K. Igarashi ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Binding Protein ◽  
Protein A ◽  
Xenopus Laevis Oocytes
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