scholarly journals Inflammation and Mechanical Stress Stimulate Osteogenic Differentiation of Human Aortic Valve Interstitial Cells

2018 ◽  
Vol 9 ◽  
Author(s):  
Maria Bogdanova ◽  
Aleksandra Kostina ◽  
Katarina Zihlavnikova Enayati ◽  
Arsenii Zabirnyk ◽  
Anna Malashicheva ◽  
...  
Keyword(s):  
Aortic Valve ◽  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Interstitial Cells ◽  
Valve Interstitial Cells ◽  
Human Aortic Valve

scholarly journals miRNA Expression Profiling Uncovers a Role of miR-139-5p in Regulating the Calcification of Human Aortic Valve Interstitial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Fan Zhang ◽  
Naixuan Cheng ◽  
Yingchun Han ◽  
Congcong Zhang ◽  
Haibo Zhang
Keyword(s):  
Aortic Valve ◽  
Osteogenic Differentiation ◽  
Mirna Expression ◽  
Structural Heart Disease ◽  
Interstitial Cells ◽  
Calcific Aortic Valve Disease ◽  
Valve Interstitial Cells ◽  
Functional Studies ◽  
Human Aortic Valve ◽  
Osteogenic Gene

Calcific aortic valve disease (CAVD) is the most common structural heart disease, and the morbidity is increased with elderly population. Several microRNAs (miRNAs) have been identified to play crucial roles in CAVD, and numerous miRNAs are still waiting to be explored. In this study, the miRNA expression signature in CAVD was analyzed unbiasedly by miRNA-sequencing, and we found that, compared with the normal control valves, 152 miRNAs were upregulated and 186 miRNAs were downregulated in calcified aortic valves. The functions of these differentially expressed miRNAs were associated with cell differentiation, apoptosis, adhesion and immune response processes. Among downregulated miRNAs, the expression level of miR-139-5p was negatively correlated with the osteogenic gene RUNX2, and miR-139-5p was also downregulated during the osteogenic differentiation of primary human aortic valve interstitial cells (VICs). Subsequent functional studies revealed that miR-139-5p overexpression inhibited the osteogenic differentiation of VICs by negatively modulating the expression of pro-osteogenic gene FZD4 and CTNNB1. In conclusion, these results suggest that miR-139-5p plays an important role in osteogenic differentiation of VICs via the Wnt/β-Catenin pathway, which may further provide a new therapeutic target for CAVD.

Novel mechanisms for osteogenic differentiation of human aortic valve interstitial cells

2020 ◽  
Vol 159 (5) ◽  
pp. 1742-1753.e7 ◽  
Author(s):  
Yongjun Wang ◽  
Xiaoxiong Xiao ◽  
Tingwen Zhou ◽  
Dong Han ◽  
Nianguo Dong
Keyword(s):  
Aortic Valve ◽  
Osteogenic Differentiation ◽  
Interstitial Cells ◽  
Valve Interstitial Cells ◽  
Human Aortic Valve

3D Contractile Responses of Normal and Diseased Human Aortic Valve Interstitial Cells

2021 ◽  
Vol 5 (sup1) ◽  
pp. 1-1
Author(s):  
Alex Khang ◽  
Chiara Camillo ◽  
Giovanni Ferrari ◽  
Michael S. Sacks
Keyword(s):  
Aortic Valve ◽  
Interstitial Cells ◽  
Contractile Responses ◽  
Valve Interstitial Cells ◽  
Human Aortic Valve

Phospholipids accumulation and calcification in cultured primary human aortic valve interstitial cells: New insights revealed by confocal Raman imaging

2019 ◽  
Vol 51 (1) ◽  
pp. 104-114
Author(s):  
Krzysztof Czamara ◽  
Magdalena Kopytek ◽  
Malgorzata Szulczewska ◽  
Agnieszka Kaczor ◽  
Joanna Natorska
Keyword(s):  
Aortic Valve ◽  
Interstitial Cells ◽  
Raman Imaging ◽  
Valve Interstitial Cells ◽  
Confocal Raman ◽  
Human Aortic Valve
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