scholarly journals The Effects of Photobiomodulation of 808 nm Diode Laser Therapy at Higher Fluence on the in Vitro Osteogenic Differentiation of Bone Marrow Stromal Cells

2018 ◽  
Vol 9 ◽  
Author(s):  
Andrea Amaroli ◽  
Dimitrios Agas ◽  
Fulvio Laus ◽  
Vincenzo Cuteri ◽  
Reem Hanna ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Laser Therapy ◽  
Diode Laser ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells

scholarly journals Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool forIn VitroOsteogenesis and Bone Tissue Engineering Approaches

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Ute Hempel ◽  
Katrin Müller ◽  
Carolin Preissler ◽  
Carolin Noack ◽  
Sabine Boxberger ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Peroxisome Proliferator ◽  
Differentiation Potential

Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesisin vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated proteinγ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts.

In Vitro Study of a Novel Oxysterol for Osteogenic Differentiation on Rabbit Bone Marrow Stromal Cells

2013 ◽  
Vol 132 (1) ◽  
pp. 70e-80e ◽  
Author(s):  
Akishige Hokugo ◽  
Sarah Sorice ◽  
Anisa Yalom ◽  
James C. Lee ◽  
Andrew Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
In Vitro Study ◽  
Marrow Stromal Cells ◽  
Vitro Study ◽  
Rabbit Bone

scholarly journals Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

2008 ◽  
Vol 26 (5) ◽  
pp. 713-720 ◽  
Author(s):  
Lijin Zou ◽  
Xuenong Zou ◽  
Li Chen ◽  
Haisheng Li ◽  
Tina Mygind ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells

Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro

2002 ◽  
Vol 85 (4) ◽  
pp. 737-746 ◽  
Author(s):  
Oliver Frank ◽  
Manuel Heim ◽  
Marcel Jakob ◽  
Andrea Barbero ◽  
Dirk Schäfer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Pcr Analysis ◽  
Rt Pcr

Effects of extracellular calcium on viability and osteogenic differentiation of bone marrow stromal cells in vitro

Human Cell ◽  
2013 ◽  
Vol 26 (3) ◽  
pp. 114-120 ◽  
Author(s):  
Shaowen Cheng ◽  
Wei Wang ◽  
Zhongqin Lin ◽  
Ping Zhou ◽  
Xiaolei Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Extracellular Calcium ◽  
Marrow Stromal Cells

In vitro study of osteogenic differentiation of bone marrow stromal cells on heat-treated porcine trabecular bone blocks

Biomaterials ◽  
2004 ◽  
Vol 25 (3) ◽  
pp. 527-535 ◽  
Author(s):  
Su-A Park ◽  
Jung-Woog Shin ◽  
Young-Il Yang ◽  
Young-Kon Kim ◽  
Ki-Dong Park ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Trabecular Bone ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
In Vitro Study ◽  
Marrow Stromal Cells ◽  
Vitro Study ◽  
Heat Treated
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