scholarly journals Calcium Sensing Receptors Mediate Local Inhibitory Reflexes Evoked by L-Phenylalanine in Guinea Pig Jejunum

2017 ◽  
Vol 8 ◽  
Author(s):  
Rachel M. Gwynne ◽  
Kenny D. K. N. Ly ◽  
Laura J. Parry ◽  
Joel C. Bornstein
Keyword(s):  
Guinea Pig ◽  
Calcium Sensing ◽  
Pig Jejunum

The relationship between motor activity and transmural potential difference in the guinea pig intestine in vitro: is there a neural link?

1986 ◽  
Vol 64 (7) ◽  
pp. 993-998 ◽  
Author(s):  
Beverley Greenwood ◽  
Stephanie Diamant ◽  
J. S. Davison
Keyword(s):  
Guinea Pig ◽  
Motor Activity ◽  
Colonic Motility ◽  
Potential Difference ◽  
Histological Data ◽  
The Relationship ◽  
Spontaneous Fluctuations ◽  
Pig Jejunum

The aim of the experiments was to examine, in vitro, the role of the enteric nervous system in the relationship between motor activity and transmural potential difference (PD) in the guinea pig jejunum and colon using the nerve blocking agents tetrodotoxin (TTX) and aconitine. Histological data showed that perfusion of the intestinal segments with gassed Hepes solution was essential for the maintenance of transmural PD. Disruption of the mucosa was associated with a loss of spontaneous fluctuations in transmural PD without any loss of spontaneous motor activity. Under spontaneous conditions, a neural pathway exists linking jejunal and colonic motility with transmural PD. However, in some cases a mechanical link was also apparent, as an attenuated TTX and aconitine–resistant component.

INFLUENCE OF OVINE PROLACTIN ON TRANSPORT OF FLUID AND SODIUM CHLORIDE BY THE MAMMALIAN INTESTINE AND GALL BLADDER

1974 ◽  
Vol 63 (2) ◽  
pp. 311-317 ◽  
Author(s):  
J. R. MAINOYA ◽  
H. A. BERN ◽  
J. W. REGAN
Keyword(s):  
Gall Bladder ◽  
Guinea Pig ◽  
Rat Colon ◽  
Guinea Pig Ileum ◽  
Rat Ileum ◽  
Nacl Absorption ◽  
Ovine Prolactin ◽  
Mammalian Intestine ◽  
Pig Jejunum ◽  
Everted Sacs

SUMMARY Mucosal fluid, sodium and chloride transfer were measured in everted sacs of rat, guinea-pig and hamster jejunum, and in rat ileum and colon, and in guinea-pig gall bladder. After treatment of the animal with ovine prolactin, a highly significant enhancement of fluid and NaCl absorption was observed in rat, hamster and guinea-pig jejunum. Prolactin treatment caused a significant increase in fluid and NaCl transfer in rat ileum, but not in guinea-pig ileum or rat colon. Prolactin administration had no consistent effect on fluid and NaCl absorption by the guinea-pig gall bladder. The several regions of the mammalian gut appear to differ in their responsiveness to prolactin.

P4.23 Cholera toxin suppresses fatty acid induced motor activity in the guinea-pig jejunum

2009 ◽  
Vol 149 (1-2) ◽  
pp. 102
Author(s):  
C. Fung ◽  
M. Ellis ◽  
R.M. Gwynne ◽  
J.C. Bornstein
Keyword(s):  
Fatty Acid ◽  
Guinea Pig ◽  
Motor Activity ◽  
Cholera Toxin ◽  
Pig Jejunum

Activation of neuronal SST1 and SST2 receptors decreases neurogenic secretion in the guinea-pig jejunum

2010 ◽  
Vol 22 (11) ◽  
pp. 1209-e317 ◽  
Author(s):  
J. P. P. Foong ◽  
L. J Parry ◽  
J. C Bornstein
Keyword(s):  
Guinea Pig ◽  
Pig Jejunum

Biomechanical remodeling of obstructed guinea pig jejunum with reference to the zero-stress state

2006 ◽  
Vol 39 ◽  
pp. S392
Author(s):  
J Yang ◽  
J. Zhao ◽  
D. Liao ◽  
P. Kunwald ◽  
H. Gregersen
Keyword(s):  
Stress State ◽  
Guinea Pig ◽  
Pig Jejunum

Volume regulation initiated by Na(+)-nutrient cotransport in isolated mammalian villus enterocytes

1991 ◽  
Vol 260 (1) ◽  
pp. G26-G33 ◽  
Author(s):  
R. J. MacLeod ◽  
J. R. Hamilton
Keyword(s):  
Guinea Pig ◽  
Volume Regulation ◽  
Brush Border Membrane ◽  
Regulatory Volume Decrease ◽  
Membrane Vesicles ◽  
Relative Volume ◽  
Cell Shrinkage ◽  
K Channels ◽  
Volume Decrease ◽  
Pig Jejunum

We assessed ion transport during regulatory volume decrease (RVD) in jejunal villus enterocytes, isolated in suspension from guinea pig jejunum and swollen by exposure to L-alanine (L-Ala) or D-glucose (D-Glc) in the presence of Na+. Cell volume was measured electronically. Relative volume of cells (rel vol: cell vol/isotonic vol) within 1 min of L-Ala (20 mM) addition increased (1.10 +/- 0.03, P less than 0.005), but by 5 min there was no difference between cells in L-Ala or 20 mM D-Ala (0.95 +/- 0.02). Cell shrinkage after maximal swelling was greater with L-Ala than with D-Ala (14 +/- 4 vs. 2 +/- 1%, P less than 0.01). Initial swelling generated by L-Ala required extracellular Na+ (P less than 0.02). Volume increased 30 s after D-Glc (20 mM), and cells were larger than cells treated with L-Glc (1.04 +/- 0.01 vs. 0.95 +/- 0.01, P less than 0.001); subsequent cell shrinkage was complete in 2 min (8 +/- 2%, P less than 0.05). Swelling generated by methyl alpha-D-glucoside was prevented by 0.1 mM phloridzin (P less than 0.05). RVD after D-Glc swelling was prevented by inhibitors of K+ channels, 5 mM Ba2+ (P less than 0.001), 100 microM quinine (P less than 0.005), or 25 mM TEA (P less than 0.02), but the same inhibitors completely prevented L-Ala swelling. All inhibitors had no effect on L-Ala uptake into brush-border membrane vesicles in presence of Na+ gradient.(ABSTRACT TRUNCATED AT 250 WORDS)

Subcellular Distribution of Radio-Labelled Iron during Intestinal Absorption in Guinea-Pig Enterocytes with Special Reference to the Mitochondrial Localization of the Iron

1979 ◽  
Vol 56 (2) ◽  
pp. 179-188 ◽  
Author(s):  
J. M. P. Hopkins ◽  
T. J. Peters
Keyword(s):  
Guinea Pig ◽  
Density Gradient Centrifugation ◽  
Early Stage ◽  
Gradient Centrifugation ◽  
Intestinal Transport ◽  
Subcellular Fractionation ◽  
Mitochondrial Fraction ◽  
Mitochondrial Localization ◽  
The Matrix ◽  
Pig Jejunum

1. 59Fe-labelled ferric chloride was introduced into tied loops of guinea-pig jejunum. 2. After 5–30 min the loop was removed, the enterocytes were isolated, homogenized and subjected to analytical subcellular fractionation. 3. Uptake of 59Fe was extremely rapid and after 5 min 45% of the radioactivity sedimented in the mitochondrial fraction. 4. Density gradient centrifugation indicated that approximately 80% of this radioactivity was associated with the mitochondria themselves; the remainder was in brush-border fragments. 5. Selective disruption of the mitochondria demonstrates that the iron is localized to the matrix and the inner membrane, indicating transport of the absorbed iron into the organelle. 6. It is suggested that mitochondria are actively implicated at an early stage in the intestinal transport of iron.

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