scholarly journals Inflammatory Effects of Menthol vs. Non-menthol Cigarette Smoke Extract on Human Lung Epithelial Cells: A Double-Hit on TRPM8 by Reactive Oxygen Species and Menthol

2017 ◽  
Vol 8 ◽  
Author(s):  
An-Hsuan Lin ◽  
Meng-Han Liu ◽  
Hsin-Kuo B. Ko ◽  
Diahn-Warng Perng ◽  
Tzong-Shyuan Lee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Human Lung ◽  
Cigarette Smoke Extract ◽  
Lung Epithelial Cells ◽  
Oxygen Species ◽  
Menthol Cigarette ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

scholarly journals High Glucose Promotes Tumor Invasion and Increases Metastasis-Associated Protein Expression in Human Lung Epithelial Cells by Upregulating Heme Oxygenase-1 via Reactive Oxygen Species or the TGF-β1/PI3K/Akt Signaling Pathway

2015 ◽  
Vol 35 (3) ◽  
pp. 1008-1022 ◽  
Author(s):  
Xiaowen Kang ◽  
Fanwu Kong ◽  
Xiaomei Wu ◽  
Yeping Ren ◽  
Shijie Wu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Epithelial Cells ◽  
Protein Expression ◽  
Human Lung ◽  
Reactive Oxygen ◽  
Lung Epithelial Cells ◽  
Oxygen Species ◽  
Lung Epithelial ◽  
Tgf Β1 ◽  
Human Lung Epithelial Cells

Background: Growing evidence indicates that heme oxygenase-1 (HO-1) is up-regulated in malignancies and subsequently alters tumor aggressiveness and various cancer-related factors, such as high glucose (HG) levels. HO-1 expression can be induced when glucose concentrations are above 25 mM; however, the role of HO-1 in lung cancer patients with diabetes remains unknown. Therefore, in this study we investigated the promotion of tumor cell invasion and the expression of metastasis-associated proteins by inducing the up-regulation of HO-1 expression by HG treatment in A549 human lung epithelial cells. Methods: The expression of HO-1and metastasis-associated protein expression was explored by western blot analysis. HO-1 enzymatic activity, reactive oxygen species (ROS) production and TGF-β1 production were examined by ELISA. Invasiveness was analyzed using a Transwell chamber. Results: HG treatment of A549 cells induced an increase in HO-1 expression, which was mediated by the HG-induced generation of reactive oxygen species (ROS) and transforming growth factor-β1 (TGF-β1) in a concentration- and time-dependent manner. Following the increase in HO-1 expression, the enzymatic activity of HO-1 also increased in HG-treated cells. Pretreatment with N-acetyl-L-cysteine (NAC) or with phosphatidylinositol 3-kinase (PI3K)/Akt inhibitors attenuated the HG-induced increase in HO-1 expression. HG treatment of A549 cells enhanced the invasion potential of these cells, as shown with a Transwell assay, and increased metastasis-associated protein expression. However, HO-1 siRNA transfection significantly decreased these capabilities. Conclusion: this study is the first to demonstrate that HG treatment of A549 human lung epithelial cells promotes tumor cell invasion and increases metastasis-associated protein expression by up-regulating HO-1 expression via ROS or the TGF-β1/PI3K/Akt signaling pathway.

Effects of Mainstream Cigarette Smoke on the Global Metabolome of Human Lung Epithelial Cells

2009 ◽  
Vol 22 (3) ◽  
pp. 492-503 ◽  
Author(s):  
Suryanarayana V. Vulimiri ◽  
Manoj Misra ◽  
Jonathan T. Hamm ◽  
Matthew Mitchell ◽  
Alvin Berger
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Mainstream Cigarette Smoke ◽  
Human Lung Epithelial Cells
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