scholarly journals Energetic Effects of Pre-hatch Albumen Removal on Embryonic Development and Early Ontogeny in Gallus gallus

2017 ◽  
Vol 7 ◽  
Author(s):  
Isaac Peña-Villalobos ◽  
Gabriela Piriz ◽  
Verónica Palma ◽  
Pablo Sabat
2019 ◽  
Vol 98 (8) ◽  
pp. 3278-3291 ◽  
Author(s):  
Shanshan Li ◽  
Shibin Bai ◽  
Xia Qin ◽  
Junpeng Zhang ◽  
David M. Irwin ◽  
...  

PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0196973 ◽  
Author(s):  
Chompunut Lumsangkul ◽  
Yang-Kwang Fan ◽  
Shen-Chang Chang ◽  
Jyh-Cherng Ju ◽  
Hsin-I. Chiang

2010 ◽  
Vol 91 (6) ◽  
pp. 1281-1287 ◽  
Author(s):  
Alexandre Alonso-Fernández ◽  
Alba Ruth Vergara ◽  
Fran Saborido-Rey

Knowledge on early ontogeny of Trisopterus luscus is scarce and incomplete. This paper describes the first successful attempt ever at achieving the natural spawning in captivity for this species. The reproductive specimens used for this study were obtained in the Ría de Vigo in September 2008 and subsequently transported to the facilities of the Institute of Marine Research (CSIC). The spawning frequency was variable among females, 1.8–4.6 days, but no size-dependent trend was determined. The average batch size, i.e. the number of eggs released per batch, has proven significantly dependent on female length and weight. In the present study the complete embryonic development in controlled conditions (sand–filtered seawater at ~13°C, with natural photoperiod), from egg to hatched larva, has been described for the first time ever. The development of pouting eggs was divided into six stages, based on the artificially-reared material. Pouting eggs are pelagic with a smooth, clear and spherical chorion, and a homogeneous yolk. The perivitelline space is narrow and oil globules are absent. Live eggs fertilized were 0.95–1.10 mm in diameter. The embryo hatches as a yolk-sac larva with closed mouth and gut. The pouting eggs hatched during the latter half of the fifth day.


Author(s):  
Inayah Yasir ◽  
Jian G. Qin

The present study describes the embryonic development and early ontogeny of Amphiprion ocellaris from fertilization to post hatching. Anemonefish spontaneously spawned at 27–28°C. The newly laid eggs were orange in colour and elliptical in shape (1.8×0.8 mm). Melanin appeared as a black mass situated at the vegetal pole in mature eggs. This is rarely seen in eggs of other fish species. We documented developmental times at 27–28°C to egg activation (0.5 h), cleavage (4 h), blastula (11.5 h), gastrula (20 h), neurula (24.5 h), somite (28.5 h), turnover (72 h), blood formation (113 h) and internal ear and jaw formation (144 h). Hatching occurred 152 h after fertilization. On day 4, the eye buds were pigmented and melanophores formed on the ventral surface of the embryo. Internal ear and gill formation were completed on day 5 and coincided with movement of the opercula and pectoral fins. The mouth formed on day 6 and the digestive tract appeared on day 7. By day 10, the yolk was fully absorbed and a substantial amount of food was observed in the gut. Dark and orange pigments were dispersed and aggregated through muscle contractions by day 14, but red pigments did not appear until the fish were three months old. This study contributes to a further understanding of the embryology and the early ontogeny of damselfish and may help improve the culture of coral reef fish.


2013 ◽  
Vol 76 (8) ◽  
pp. 803-803 ◽  
Author(s):  
Dayane Alcântara ◽  
Marcio N. Rodrigues ◽  
André L. R. Franciolli ◽  
Erika T. Da Fonseca ◽  
Fernanda M. O. Silva ◽  
...  

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5094 ◽  
Author(s):  
Luzie Jessl ◽  
Rebecca Lenz ◽  
Fabian G. Massing ◽  
Jessica Scheider ◽  
Jörg Oehlmann

Since it is known that environmental contaminants have the potential to cause endocrine disorders in humans and animals, there is an urgent need for in vivo tests to assess possible effects of these endocrine disrupting chemicals (EDCs). Although there is no standardized guideline, the avian embryo has proven to be particularly promising as it responds sensitively to a number of EDCs preferentially impacting the reproductive axis. In the present study we examined the effects of in ovo exposure to fulvestrant and tamoxifen as antiestrogenic model compounds and co-exposure to both substances and the potent estrogen 17α-ethinylestradiol (EE2) regarding sex differentiation and embryonic development of the domestic fowl (Gallus gallus domesticus). The substances were injected into the yolk of fertilized eggs on embryonic day 1. On embryonic day 19 sex genotype and phenotype were determined, followed by gross morphological and histological examination of the gonads. Sole EE2-treatment (20 ng/g egg) particularly affected male gonads and resulted in an increased formation of female-like gonadal cortex tissue and a reduction of seminiferous tubules. In ovo exposure to tamoxifen (0.1/1/10 µg/g egg) strongly impaired the differentiation of female gonads, led to a significant size reduction of the left ovary and induced malformations of the ovarian cortex, while fulvestrant (0.1/1/10 µg/g egg) did not affect sexual differentiation. However, both antiestrogens were able to antagonize the feminizing effects of EE2in genetic males when administered simultaneously. Since both estrogens and antiestrogens induce concentration-dependent morphological alterations of the sex organs, the chick embryo can be regarded as a promising model for the identification of chemicals with estrogenic and antiestrogenic activity.


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