scholarly journals Cutting a Long Intron Short: Recursive Splicing and Its Implications

2016 ◽  
Vol 7 ◽  
Author(s):  
Theodore Georgomanolis ◽  
Konstantinos Sofiadis ◽  
Argyris Papantonis
Keyword(s):  
Recursive Splicing

scholarly journals Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Tetsutaro Hayashi ◽  
Haruka Ozaki ◽  
Yohei Sasagawa ◽  
Mana Umeda ◽  
Hiroki Danno ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Full Length ◽  
Total Rna ◽  
Recursive Splicing

scholarly journals Recursive splicing in long vertebrate genes

Nature ◽  
2015 ◽  
Vol 521 (7552) ◽  
pp. 371-375 ◽  
Author(s):  
Christopher R. Sibley ◽  
Warren Emmett ◽  
Lorea Blazquez ◽  
Ana Faro ◽  
Nejc Haberman ◽  
...  
Keyword(s):  
Recursive Splicing

scholarly journals Subdivision of Large Introns in Drosophila by Recursive Splicing at Nonexonic Elements

Genetics ◽  
2005 ◽  
Vol 170 (2) ◽  
pp. 661-674 ◽  
Author(s):  
James M. Burnette ◽  
Etsuko Miyamoto-Sato ◽  
Marc A. Schaub ◽  
Jamie Conklin ◽  
A. Javier Lopez
Keyword(s):  
Recursive Splicing

scholarly journals The temporal landscape of recursive splicing during Pol II transcription elongation in human cells

PLoS Genetics ◽  
2018 ◽  
Vol 14 (8) ◽  
pp. e1007579 ◽  
Author(s):  
Xiao-Ou Zhang ◽  
Yu Fu ◽  
Haiwei Mou ◽  
Wen Xue ◽  
Zhiping Weng
Keyword(s):  
Transcription Elongation ◽  
Human Cells ◽  
Pol Ii ◽  
Recursive Splicing

scholarly journals Molecular and genetic dissection of recursive splicing

2021 ◽  
Vol 5 (1) ◽  
pp. e202101063
Author(s):  
Brian Joseph ◽  
Chaz Scala ◽  
Shu Kondo ◽  
Eric C Lai
Keyword(s):  
Complete Suppression ◽  
Genetic Dissection ◽  
Splice Acceptor ◽  
Splice Donor ◽  
Cryptic Exon ◽  
Exon Expression ◽  
Multiple Factors ◽  
Exon Usage ◽  
Recursive Splicing

Intronic ratchet points (RPs) are abundant within long introns in the Drosophila genome and consist of juxtaposed splice acceptor and splice donor (SD) sites. Although they appear to encompass zero-nucleotide exons, we recently clarified that intronic recursive splicing (RS) requires a cryptic exon at the RP (an RS-exon), which is subsequently always skipped and thus absent from mRNA. In addition, Drosophila encodes a smaller set of expressed exons bearing features of RS. Here, we investigate mechanisms that regulate the choice between RP and RS-exon SDs. First, analysis of Drosophila RP SD mutants demonstrates that SD competition suppresses inclusion of cryptic exons in endogenous contexts. Second, characterization of RS-exon reporters implicates exonic sequences as influencing choice of RS-exon usage. Using RS-exon swap and mutagenesis assays, we show exonic sequences can determine RS-exon inclusion. Finally, we provide evidence that splicing can suppress utilization of RP SDs to enable RS-exon expression. Overall, multiple factors can influence splicing of Drosophila RS-exons, which usually result in their complete suppression as zero-nucleotide RPs, but occasionally yield translated RS-exons.

scholarly journals SplicePie: a novel analytical approach for the detection of alternative, non-sequential and recursive splicing

2015 ◽  
Vol 43 (12) ◽  
pp. e80-e80 ◽  
Author(s):  
Irina Pulyakhina ◽  
Isabella Gazzoli ◽  
Peter A.C. ’t Hoen ◽  
Nisha Verwey ◽  
Johan den Dunnen ◽  
...  
Keyword(s):  
Analytical Approach ◽  
Recursive Splicing

scholarly journals SplicePie: a novel analytical approach for the detection of alternative, non-sequential and recursive splicing

2015 ◽  
Vol 43 (22) ◽  
pp. 11068-11068 ◽  
Author(s):  
Irina Pulyakhina ◽  
Isabella Gazzoli ◽  
Peter A.C.'t Hoen ◽  
Nisha Verwey ◽  
Johan T. den Dunnen ◽  
...  
Keyword(s):  
Analytical Approach ◽  
Recursive Splicing
Sign in / Sign up

Export Citation Format

Share Document