scholarly journals Controlled Reperfusion Strategies Improve Cardiac Hemodynamic Recovery after Warm Global Ischemia in an Isolated, Working Rat Heart Model of Donation after Circulatory Death (DCD)

2016 ◽  
Vol 7 ◽  
Author(s):  
Emilie Farine ◽  
Petra Niederberger ◽  
Rahel K. Wyss ◽  
Natalia Méndez-Carmona ◽  
Brigitta Gahl ◽  
...  
Keyword(s):  
Rat Heart ◽  
Global Ischemia ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Circulatory Death ◽  
Working Rat Heart ◽  
Controlled Reperfusion

Resistance of Cardiac Mitochondria to Ischemia and Reperfusion in an Isolated Working Rat Heart Model of Donation After Circulatory Death

2017 ◽  
Vol 101 ◽  
pp. S54
Author(s):  
Rahel K. Wyss ◽  
Natalia Méndez Carmona ◽  
Maria-Nieves Sanz ◽  
Maria Arnold ◽  
Thierry P. Carrel ◽  
...  
Keyword(s):  
Rat Heart ◽  
Ischemia And Reperfusion ◽  
Cardiac Mitochondria ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Circulatory Death ◽  
Working Rat Heart

High pre-ischemic fatty acid levels decrease cardiac recovery in an isolated rat heart model of donation after circulatory death

Metabolism ◽  
2017 ◽  
Vol 71 ◽  
pp. 107-117 ◽  
Author(s):  
Petra Niederberger ◽  
Emilie Farine ◽  
Maria Arnold ◽  
Rahel K. Wyss ◽  
Maria N. Sanz ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Cardiac Recovery ◽  
Circulatory Death ◽  
Isolated Rat

scholarly journals Cardioprotective reperfusion strategies differentially affect mitochondria: Studies in an isolated rat heart model of donation after circulatory death (DCD)

2018 ◽  
Vol 19 (2) ◽  
pp. 331-344 ◽  
Author(s):  
Maria N. Sanz ◽  
Emilie Farine ◽  
Petra Niederberger ◽  
Natalia Méndez-Carmona ◽  
Rahel K. Wyss ◽  
...  
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Circulatory Death ◽  
Isolated Rat

Characterization of Endothelial Function Following Various Ischemic Durations in an Isolated Rat Heart Model of Donation After Circulatory Death

2017 ◽  
Vol 101 ◽  
pp. S53
Author(s):  
Natalia Méndez Carmona ◽  
Rahel K. Wyss ◽  
Maria Arnold ◽  
Hendrik T. Tevaearai Stahel ◽  
Thierry P. Carrel ◽  
...  
Keyword(s):  
Endothelial Function ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Circulatory Death ◽  
Isolated Rat

Molecular and Metabolic Mechanisms of Mechanical Mostconditioning in an Isolated Rat Heart Model of Donation after Circulatory Death

2017 ◽  
Vol 101 ◽  
pp. S52
Author(s):  
Maria Arnold ◽  
Natalia Méndez Carmona ◽  
Patrik Gulac ◽  
Rahel K. Wyss ◽  
Hendrik Tevaearai Stahel ◽  
...  
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Circulatory Death ◽  
Isolated Rat

scholarly journals Pre-ischemic Lactate Levels Affect Post-ischemic Recovery in an Isolated Rat Heart Model of Donation After Circulatory Death (DCD)

2021 ◽  
Vol 8 ◽  
Author(s):  
Maria Arnold ◽  
Adrian Segiser ◽  
Selianne Graf ◽  
Natalia Méndez-Carmona ◽  
Maria N. Sanz ◽  
...  
Keyword(s):  
Rat Heart ◽  
Left Ventricular ◽  
Isolated Rat Heart ◽  
Mitochondrial Calcium ◽  
Donation After Circulatory Death ◽  
Heart Model ◽  
Early Reperfusion ◽  
Lactate Levels ◽  
Circulatory Death ◽  
Isolated Rat

Introduction: Donation after circulatory death (DCD) could substantially improve donor heart availability. In DCD, the heart is not only exposed to a period of warm ischemia, but also to a damaging pre-ischemic phase. We hypothesized that the DCD-relevant pre-ischemic lactate levels negatively affect the post-ischemic functional and mitochondrial recovery in an isolated rat heart model of DCD.Methods: Isolated, working rat hearts underwent 28.5′ of global ischemia and 60′ of reperfusion. Prior to ischemia, hearts were perfused with one of three pre-ischemic lactate levels: no lactate (0 Lac), physiologic lactate (0.5 mM; 0.5 Lac), or DCD-relevant lactate (1 mM; 1 Lac). In a fourth group, an inhibitor of the mitochondrial calcium uniporter was added in reperfusion to 1 Lac hearts (1 Lac + Ru360).Results: During reperfusion, left ventricular work (heart rate-developed pressure product) was significantly greater in 0.5 Lac hearts compared to 0 Lac or 1 Lac. In 1 vs. 0.5 Lac hearts, in parallel with a decreased function, cellular and mitochondrial damage was greater, tissue calcium content tended to increase, while oxidative stress damage tended to decrease. The addition of Ru360 to 1 Lac hearts partially abrogated the negative effects of the DCD-relevant pre-ischemic lactate levels (greater post-ischemic left ventricular work and less cytochrome c release in 1 Lac+Ru360 vs. 1 Lac).Conclusion: DCD-relevant levels of pre-ischemic lactate (1 mM) reduce contractile, cellular, and mitochondrial recovery during reperfusion compared to physiologic lactate levels. Inhibition of mitochondrial calcium uptake during early reperfusion improves the post-ischemic recovery of 1 Lac hearts, indicating calcium overload as a potential therapeutic reperfusion target for DCD hearts.

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