scholarly journals Cx43-hemichannel function and regulation in physiology and pathophysiology: insights from the bovine corneal endothelial cell system and beyond

2014 ◽  
Vol 5 ◽  
Author(s):  
Catheleyne D'hondt ◽  
Jegan Iyyathurai ◽  
Bernard Himpens ◽  
Luc Leybaert ◽  
Geert Bultynck
Keyword(s):  
Endothelial Cell ◽  
Cell System ◽  
Corneal Endothelial Cell ◽  
Bovine Corneal Endothelial Cell

Fluid transport across cultured bovine corneal endothelial cell monolayers

1992 ◽  
Vol 262 (1) ◽  
pp. C98-C103 ◽  
Author(s):  
P. Narula ◽  
M. Xu ◽  
K. Y. Kuang ◽  
R. Akiyama ◽  
J. Fischbarg
Keyword(s):  
Endothelial Cell ◽  
Electrical Resistance ◽  
Corneal Endothelium ◽  
Fluid Transport ◽  
Corneal Endothelial Cell ◽  
Potential Difference ◽  
Cell Monolayers ◽  
Transport Fluid ◽  
Bovine Corneal Endothelial Cell

The mammalian corneal endothelium is known to transport fluid from the stromal compartment to the aqueous humor, thereby maintaining corneal transparency. Corneal endothelial cells have been cultured for some years now, but whether they preserve their in vivo ability to actively transport fluid is not known. We have now grown bovine corneal endothelial cell monolayers (BCECM) on permeable substrates (Transwell) and report that, just like their counterparts in vivo, these cultured cells pump fluid from the basal to the apical compartment and display measurable electrical resistance and potential difference across the monolayer. BCECM were grown on collagen-treated permeable supports using Dulbecco's modified Eagle's medium (DMEM)/20% fetal bovine serum with antibiotics. Cells grew to confluence in 5-7 days and displayed polygonal shape. Only cells from passages 1-3 were utilized. Inserts were fitted directly into Lucite chambers specially built. The rate of fluid pumping by BCECM was 3.96 +/- 0.49 (SE) microliter.h-1.cm-2 (n = 13) and could be measured continuously for several hours; fluid pumping was inhibited by 0.2 mM amiloride. The specific electrical resistance of the monolayers was 180 +/- 22 omega.cm2 (n = 11). A mean electrical potential difference of 63.8 +/- 3.7 microV (n = 15, range 40-100 microV, apical side negative) was recorded across the monolayers in DMEM. The availability of the commercial inserts makes this procedure practical; as a consequence, the rate of fluid transport by cultured corneal endothelium has been quantitated for the first time. This method can now be extended to other cultured layers.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Myopia, corneal endothelial cell density and morphology in a Japanese population-based cross-sectional study: the JPHC-NEXT Eye Study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Naohiko Aketa ◽  
Miki Uchino ◽  
Motoko Kawashima ◽  
Yuichi Uchino ◽  
Kenya Yuki ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Density ◽  
Contact Lenses ◽  
Population Based ◽  
Cross Sectional Study ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Density ◽  
Sectional Study ◽  
Cross Sectional ◽  
Corneal Endothelial Cell Density

AbstractThis population-based cross-sectional study was performed to determine the mean corneal endothelial cell density (ECD), coefficient of variation (CV), and hexagonality (HEX), and their associations with myopia in Japanese adults living in Chikusei city. Of 7109 participants with available data, 5713 (2331 male and 3382 female) participants were eligible for analysis. After assessing the relationship between participant characteristics and spherical equivalent refraction (SER), the association of SER with the abnormal value of ECD (< 2000 cells/mm), CV (≥ 0.40), and HEX (≤ 50%) were determined using the logistic regression models adjusting for potential confounders (age, intraocular pressure, keratometric power, height, and antihypertensive drug use). In male participants, there was no statistically significant relationships between SER and endothelial parameters. In female participants, compared to emmetropia, SER ≤ − 6 D had significantly higher odds ratio (OR) of having the abnormal value of CV (OR = 2.07, 95% confidence interval [CI] 1.39–3.10) and HEX (OR = 2.04, 95% CI 1.29–3.23), adjusted for potential confounders, indicating that the high myopia was associated with the abnormal values of CV and HEX. Further adjustment for contact lenses wear partly attenuated these associations. Association between the SER and ECD was not detected.

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