scholarly journals Targeting diseased tissues by pHLIP insertion at low cell surface pH

2014 ◽  
Vol 5 ◽  
Author(s):  
Oleg A. Andreev ◽  
Donald M. Engelman ◽  
Yana K. Reshetnyak
Keyword(s):  
Cell Surface ◽  
Surface Ph

scholarly journals Probe for the measurement of cell surface pH in vivo and ex vivo

2016 ◽  
Vol 113 (29) ◽  
pp. 8177-8181 ◽  
Author(s):  
Michael Anderson ◽  
Anna Moshnikova ◽  
Donald M. Engelman ◽  
Yana K. Reshetnyak ◽  
Oleg A. Andreev
Keyword(s):  
Cell Surface ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Metastatic Cancer ◽  
Surface Acidity ◽  
Measurement Tool ◽  
Mouse Tumor ◽  
Cell Surfaces ◽  
Surface Ph

We have developed a way to measure cell surface pH by positioning a pH-sensitive fluorescent dye, seminaphtharhodafluor (SNARF), conjugated to the pH low insertion peptide (pHLIP). It has been observed that many diseased tissues are acidic and that tumors are especially so. A combination of effects acidifies tumor cell interiors, and cells pump out lactic acid and protons to maintain intracellular pH, acidifying the extracellular space. Overexpression of carbonic anhydrases on cell surfaces further contributes to acidification. Thus, the pH near tumor cell surfaces is expected to be low and to increase with distance from the membrane, so bulk pH measurements will not report surface acidity. Our new surface pH-measurement tool was validated in cancer cells grown in spheroids, in mouse tumor models in vivo, and in excised tumors. We found that the surface pH is sensitive to cell glycolytic activity: the pH decreases in high glucose and increases if glucose is replaced with nonmetabolized deoxyglucose. For highly metastatic cancer cells, the pH measured at the surface was 6.7–6.8, when the surrounding external pH was 7.4. The approach is sensitive enough to detect 0.2–0.3 pH unit changes in vivo in tumors induced by i.p. injection of glucose. The pH at the surfaces of highly metastatic cells within tumors was found to be about 6.1–6.4, whereas in nonmetastatic tumors, it was 6.7–6.9, possibly creating a way to distinguish more aggressive from less aggressive tumors. Other biological roles of surface acidity may be found, now that targeted measurements are possible.

Response of cell surface pH to pCO2 and iron limitation in the marine diatom Thalassiosira weissflogii

2009 ◽  
Vol 114 (1-2) ◽  
pp. 31-36 ◽  
Author(s):  
Allen J. Milligan ◽  
Cécile E. Mioni ◽  
François M.M. Morel
Keyword(s):  
Cell Surface ◽  
Iron Limitation ◽  
Marine Diatom ◽  
Thalassiosira Weissflogii ◽  
Surface Ph

Evolutionary determinants of normal arterial plasma pH in ectothermic vertebrates

2002 ◽  
Vol 205 (5) ◽  
pp. 641-650
Author(s):  
Richard F. Burton
Keyword(s):  
Cell Surface ◽  
Interstitial Fluid ◽  
Acid Base ◽  
Arterial Pco2 ◽  
Surface Ph ◽  
Mean Values ◽  
Surface Sites ◽  
Arterial Ph ◽  
Arterial Plasma ◽  
Optimal Ph

SUMMARYMean values of normal arterial pH in different species of fish, amphibians and reptiles at 15 and 25°C, taken from the literature, are negatively correlated with arterial PCO2 and plasma [Na+]. At either temperature, the data accord with the hypothesis that extracellular acid–base homeostasis evolved to maintain an optimal pH at particular cell-surface sites that are similar in all species. These hypothetical sites bear fixed negative charges that attract H+, but which are partially screened by Na+; for the surface pH to be constant, the bulk interstitial pH should then vary inversely with [Na+], as is the case. At the same time, the bulk interstitial fluid must be more acid than arterial plasma by an amount that increases with decreasing arterial PCO2. With allowance made for additional screening by Ca2+ and Mg2+, the relevant cell-surface pH is probably approximately 6.2.

scholarly journals Litmus-Body: A Molecularly Targeted Sensor for Cell-Surface pH Measurements

ACS Sensors ◽  
2020 ◽  
Vol 5 (6) ◽  
pp. 1555-1566 ◽  
Author(s):  
Joe Chin-Hun Kuo ◽  
Marc C. Goudge ◽  
Ann E. Metzloff ◽  
Ling-Ting Huang ◽  
Marshall J. Colville ◽  
...  
Keyword(s):  
Cell Surface ◽  
Ph Measurements ◽  
Surface Ph

scholarly journals Cell surface pH imaging using poly(ethylene glycol)-phospholipid: its potential as the core structure of membrane anchored-probes

2019 ◽  
Vol 153 (6) ◽  
pp. 254-260
Author(s):  
Ryuichi Ohgaki ◽  
Yuji Teramura ◽  
Daichi Hayashi ◽  
Shushi Nagamori ◽  
Madoka Takai ◽  
...  
Keyword(s):  
Cell Surface ◽  
Ethylene Glycol ◽  
Core Structure ◽  
Poly Ethylene Glycol ◽  
Surface Ph ◽  
The Core ◽  
Ph Imaging ◽  
Poly Ethylene

scholarly journals Programmable DNA Framework Sensors for In Situ Cell-Surface pH Analysis

2021 ◽  
Vol 93 (36) ◽  
pp. 12170-12174
Author(s):  
Jingxin Liu ◽  
Weiwu Li ◽  
Rongsong Li ◽  
Xiuzhao Yin ◽  
Shiliang He ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Ph

A MOF-Shell-Confined I-Motif-Based pH Probe (MOFC-i) Strategy for Sensitive and Dynamic Imaging of Cell Surface pH

2021 ◽  
Author(s):  
Huihui Yang ◽  
Jun Chen ◽  
Yuling Liang ◽  
Yanfei Zhang ◽  
Wen Yin ◽  
...  
Keyword(s):  
Cell Surface ◽  
Dynamic Imaging ◽  
Surface Ph ◽  
Ph Probe

The glycocalyx maintains a cell surface pH nanoenvironment crucial for integrin-mediated migration of human melanoma cells

2009 ◽  
Vol 458 (6) ◽  
pp. 1069-1083 ◽  
Author(s):  
Hermann Krähling ◽  
Sabine Mally ◽  
Johannes A. Eble ◽  
Josette Noël ◽  
Albrecht Schwab ◽  
...  
Keyword(s):  
Cell Surface ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Surface Ph ◽  
Human Melanoma Cells ◽  
A Cell
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