scholarly journals Intracellular pH regulation by acid-base transporters in mammalian neurons

2014 ◽  
Vol 5 ◽  
Author(s):  
Vernon A. Ruffin ◽  
Ahlam I. Salameh ◽  
Walter F. Boron ◽  
Mark D. Parker
Keyword(s):  
Intracellular Ph ◽  
Ph Regulation ◽  
Acid Base ◽  
Intracellular Ph Regulation

Myocardial intracellular pH regulation during chloride depletion

1981 ◽  
Vol 51 (6) ◽  
pp. 1630-1634 ◽  
Author(s):  
N. C. Gonzalez ◽  
R. L. Clancy
Keyword(s):  
Intracellular Ph ◽  
Ph Regulation ◽  
Significant Negative Correlation ◽  
Myocardial Cell ◽  
Ringer Solution ◽  
Acid Base ◽  
Co2 Partial Pressure ◽  
Intracellular Ph Regulation ◽  
Chloride Depletion

The possible role of a HCO-3/Cl- transmembrane exchange as a mechanism of alkalinization in the myocardial cell was studied in isolated rabbit hearts perfused with Ringer solution. Cl- depletion was produced by replacing Cl- of the perfusate by SO2(-4) or glucuronate. Intracellular pH (pHi) was calculated both in Cl--free and Cl--containing hearts from the distribution of 14C-labeled 5′,5′-dimethyloxazolidine-2,4-dione. Acid-base alterations were produced by changing perfusate HCO-3 concentration and/or CO2 partial pressure (PCO2). Depletion of Cl- resulted in an increase in pHi for any given level of extracellular pH. Increasing PCO2 at constant perfusate HCO-3 resulted in changes in pHi and cell HCO-3 (HCO-3i) that were similar in both Cl--free and Cl--containing hearts. Increasing perfusate HCO-3 at constant PCO2 resulted in increases in pHi and HCO-3i in both Cl--free and Cl--containing preparations. When the increases in HCO-3i secondary to an increase in extracellular HCO-3 were plotted as a function of the initial HCO-3i, a significant negative correlation was observed, suggesting that the elevation of HCO-3i was influenced by the initial HCo-3i and not by the presence or absence of Cl-. It is concluded that even though HCO-3 may enter the myocardial cells in exchange for Cl- during Cl-depletion, lack of Cl- does not alter the ability of the myocardial cell to regulate its pHi. This argues against a HCO-3/Cl- exchange as a mechanism of regulation of myocardial pHi.

scholarly journals Intracellular pH regulation: characterization and functional investigation of H+ transporters in Stylophora pistillata

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Laura Capasso ◽  
Philippe Ganot ◽  
Víctor Planas-Bielsa ◽  
Sylvie Tambutté ◽  
Didier Zoccola
Keyword(s):  
Intracellular Ph ◽  
Ph Regulation ◽  
Expression Patterns ◽  
Acid Base Balance ◽  
Acid Base ◽  
Stylophora Pistillata ◽  
Intracellular Ph Regulation ◽  
Seawater Acidification ◽  
Base Balance

AbstractBackgroundReef-building corals regularly experience changes in intra- and extracellular H+concentrations ([H+]) due to physiological and environmental processes. Stringent control of [H+] is required to maintain the homeostatic acid-base balance in coral cells and is achieved through the regulation of intracellular pH (pHi). This task is especially challenging for reef-building corals that share an endosymbiotic relationship with photosynthetic dinoflagellates (family Symbiodinaceae), which significantly affect the pHiof coral cells. Despite their importance, the pH regulatory proteins involved in the homeostatic acid-base balance have been scarcely investigated in corals. Here, we report in the coralStylophora pistillataa full characterization of the genomic structure, domain topology and phylogeny of three major H+transporter families that are known to play a role in the intracellular pH regulation of animal cells; we investigated their tissue-specific expression patterns and assessed the effect of seawater acidification on their expression levels.ResultsWe identified members of the Na+/H+exchanger (SLC9), vacuolar-type electrogenic H+-ATP hydrolase (V-ATPase) and voltage-gated proton channel (HvCN) families in the genome and transcriptome ofS. pistillata. In addition, we identified a novel member of the HvCN gene family in the cnidarian subclass Hexacorallia that has not been previously described in any species. We also identified key residues that contribute to H+transporter substrate specificity, protein function and regulation. Last, we demonstrated that some of these proteins have different tissue expression patterns, and most are unaffected by exposure to seawater acidification.ConclusionsIn this study, we provide the first characterization of H+transporters that might contribute to the homeostatic acid-base balance in coral cells. This work will enrich the knowledge of the basic aspects of coral biology and has important implications for our understanding of how corals regulate their intracellular environment.

Mechanisms of intracellular pH regulation during postischemic reperfusion of diabetic rat hearts

Diabetes ◽  
1995 ◽  
Vol 44 (2) ◽  
pp. 196-202 ◽  
Author(s):  
N. Khandoudi ◽  
M. Bernard ◽  
P. Cozzone ◽  
D. Feuvray
Keyword(s):  
Intracellular Ph ◽  
Ph Regulation ◽  
Diabetic Rat ◽  
Intracellular Ph Regulation ◽  
Postischemic Reperfusion ◽  
Rat Hearts

scholarly journals The Boron & De Weer Model of Intracellular pH Regulation

2020 ◽  
Author(s):  
Rossana Occhipinti ◽  
Soroush Safaei ◽  
Peter J. Hunter ◽  
Walter F. Boron
Keyword(s):  
Cell Membrane ◽  
Intracellular Ph ◽  
Ph Regulation ◽  
Quantitative Model ◽  
Historical Background ◽  
Acid Base ◽  
Experimental Conditions ◽  
Subsequent Work ◽  
Energy Dependent ◽  
Parameter Values

The classic Boron & De Weer (1976) paper provided the first evidence of active regulation of pH} in cells by an energy-dependent acid-base transporter. These authors also developed a quantitative model --- comprising passive fluxes of acid-base equivalents across the cell membrane, intracellular reactions, and an active transport mechanism in the cell membrane (modelled as a proton pump) --- to help interpret their measurements of intracellular pH under perturbations of both extracellular CO2/HCO3- and extracellular NH3/NH4+. This Physiome paper seeks to make that model, and the experimental conditions under which it was developed, available in a reproducible and well-documented form, along with a software implementation that makes the model easy to use and understand. We have also taken the opportunity to update some of the units used in the original paper, and to provide a few parameter values that were missing in the original paper. Finally, we provide an historical background to the Boron & De Weer (1976) proposal for active pH regulation and a commentary on subsequent work that has enriched our understanding of this most basic aspect of cellular physiology.

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