scholarly journals Role of AE2 for pHi regulation in biliary epithelial cells

2014 ◽  
Vol 4 ◽  
Author(s):  
Axel R. Concepcion ◽  
María Lopez ◽  
Alberto Ardura-Fabregat ◽  
Juan F. Medina
2004 ◽  
Vol 127 (3) ◽  
pp. 903-913 ◽  
Author(s):  
Andrew P. Feranchak ◽  
R. Brian Doctor ◽  
Marlyn Troetsch ◽  
Kathryn Brookman ◽  
Sylene M. Johnson ◽  
...  

2007 ◽  
Vol 39 (10) ◽  
pp. A35-A36
Author(s):  
A. Ferrigno ◽  
V. Rizzo ◽  
F. Carlucci ◽  
A. Tabucchi ◽  
E. Boncompagni ◽  
...  

Hepatology ◽  
2008 ◽  
Vol 47 (3) ◽  
pp. 958-965 ◽  
Author(s):  
Shinji Shimoda ◽  
Kenichi Harada ◽  
Hiroaki Niiro ◽  
Tomoharu Yoshizumi ◽  
Yuji Soejima ◽  
...  

Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti

To learn more of the nature and origin of alcoholic hyalin (AH), 15 liver biopsy specimens from patients with alcoholic hepatitis were studied in detail.AH was found not only in hepatocytes but also in ductular cells (Figs. 1 and 2), although in the latter location only rarely. The bulk of AH consisted of a randomly oriented network of closely packed filaments measuring about 150 Å in width. Bundles of filaments smaller in diameter (40-90 Å) were observed along the periphery of the main mass (Fig. 1), often surrounding it in a rim-like fashion. Fine filaments were also found close to the nucleus in both hepatocytes and biliary epithelial cells, the latter even though characteristic AH was not present (Figs. 3 and 4). Dispersed among the larger filaments were glycogen, RNA particles and profiles of endoplasmic reticulum. Dilated cisternae of endoplasmic reticulum were often conspicuous around the periphery of the AH mass. A limiting membrane was not observed.


Author(s):  
W.T. Gunning ◽  
M.R. Marino ◽  
M.S. Babcock ◽  
G.D. Stoner

The role of calcium in modulating cellular replication and differentiation has been described for various cell types. In the present study, the effects of Ca++ on the growth and differentiation of cultured rat esophageal epithelial cells was investigated.Epithelial cells were isolated from esophagi taken from 8 week-old male CDF rats by the enzymatic dissociation method of Kaighn. The cells were cultured in PFMR-4 medium supplemented with 0.25 mg/ml dialyzed fetal bovine serum, 5 ng/ml epidermal growth factor, 10-6 M hydrocortisone 10-6 M phosphoethanolamine, 10-6 M ethanolamine, 5 pg/ml insulin, 5 ng/ml transferrin, 10 ng/ml cholera toxin and 50 ng/ml garamycin at 36.5°C in a humidified atmosphere of 3% CO2 in air. At weekly intervals, the cells were subcultured with a solution containing 1% polyvinylpyrrolidone, 0.01% EGTA, and 0.05% trypsin. After various passages, the replication rate of the cells in PFMR-4 medium containing from 10-6 M to 10-3 M Ca++ was determined using a clonal growth assay.


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