scholarly journals Microalgae as Potential Anti-Inflammatory Natural Product Against Human Inflammatory Skin Diseases

2020 ◽  
Vol 11 ◽  
Author(s):  
Wu-Thong Choo ◽  
Ming-Li Teoh ◽  
Siew-Moi Phang ◽  
Peter Convey ◽  
Wei-Hsum Yap ◽  
...  
Keyword(s):  
Natural Product ◽  
Skin Diseases ◽  
Inflammatory Skin Diseases ◽  
Inflammatory Skin ◽  
Anti Inflammatory

scholarly journals Immunomodulatory properties of umbilical cord blood-derived small extracellular vesicles and their therapeutic potential for inflammatory skin disorders

2021 ◽  
Author(s):  
Silvia C Rodrigues ◽  
Renato M S Cardoso ◽  
Patricia C Freire ◽  
Claudia F Gomes ◽  
Filipe V Duarte ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Extracellular Vesicles ◽  
Umbilical Cord Blood ◽  
Skin Diseases ◽  
Therapeutic Potential ◽  
Psoriatic Skin ◽  
Inflammatory Skin Diseases ◽  
Inflammatory Skin ◽  
Anti Inflammatory

Umbilical cord blood (UCB) has long been seen as a rich source of naive cells with strong regenerative potential, likely mediated by small extracellular vesicles (sEV). More recently, small extracellular vesicles (sEV), such as exosomes, have been shown to play essential roles in cell-to-cell communication, via the transport of numerous molecules, including small RNAs. Often explored for their potential as biomarkers, sEV are now known to have regenerative and immunomodulating characteristics, particularly if isolated from stem cell-rich tissues. In this study, we aim to characterize the immunomodulating properties of umbilical cord blood mononuclear cell sEV (herein referred as Exo-101), and explore their therapeutic potential for inflammatory skin diseases. Exo-101 was shown to shift macrophages toward an anti-inflammatory phenotype, which in turn exert paracrine effects on fibroblasts, despite previous inflammatory stimuli. Additionally, the incubation of PBMC with Exo-101 resulted in an reduction of total CD4+ and CD8+ T-cell proliferation and cytokine release, while specifically supporting the development of regulatory T-cells (Treg), by influencing FOXP3 expression. In a 3D model of psoriatic skin, Exo-101 reduced the expression of inflammatory and psoriatic markers IL-6, IL-8, CXCL10, COX-2, S100A7 and DEFB4. In vivo, Exo-101 significantly prevented or reversed acanthosis in imiquimod-induced psoriasis, and tendentially increased the number of Treg in skin, without having an overall impact on disease burden. This work provides evidence for the anti-inflammatory and tolerogenic effect of Exo-101, which may be harnessed for the treatment of Th17-driven inflammatory skin diseases, such as psoriasis.

scholarly journals Anti-Allergic and Anti-Inflammatory Effects of Kuwanon G and Morusin on MC/9 Mast Cells and HaCaT Keratinocytes

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 265 ◽  
Author(s):  
Seong Jin ◽  
Hyekyung Ha ◽  
Hyeun-Kyoo Shin ◽  
Chang-Seob Seo
Keyword(s):  
Mast Cells ◽  
Western Blotting ◽  
Skin Diseases ◽  
Hacat Keratinocytes ◽  
Inflammatory Skin Diseases ◽  
Histamine Production ◽  
Tnf Α ◽  
Ifn Γ ◽  
Inflammatory Skin ◽  
Anti Inflammatory

Atopic dermatitis (AD) is a prevalent chronic inflammatory skin disease. The use of immunomodulatory corticosteroids in AD treatment causes adverse side effects. Therefore, novel natural anti-inflammatory therapeutics are needed. The aim of the present study was to investigate the anti-allergic and anti-inflammatory activities of kuwanon G and morusin. To investigate the effect of kuwanon G and morusin on skin inflammation, enzyme-linked immunosorbent assays (ELISA) to quantitate secreted (RANTES/CCL5), thymus- and activation-regulated chemokine (TARC/CCL17), and macrophage-derived chemokine (MDC/CCL22) were performed, followed by Western blotting to measure the phosphorylation of signal transducer and activator of transcription 1 (STAT1) and nuclear transcription factor-κB (NF-κB) p65 in tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ)-stimulated HaCaT keratinocytes. In order to evaluate the anti-allergic effects, ELISA to quantify histamine and leukotriene C4 (LTC4) production and Western blotting to measure 5-lipoxygenase (5-LO) activation were performed using PMA and A23187-stimulated MC/9 mast cells. Kuwanon G reduced the release of RANTES/CCL5, TARC/CCL17, and MDC/CCL22 via down-regulation of STAT1 and NF-κB p65 signaling in TNF-α and IFN-γ-stimulated HaCaT keratinocytes. Kuwanon G also inhibited histamine production and 5-LO activation in PMA and A23187-stimulated MC/9 mast cells. Morusin inhibited RANTES/CCL5 and TARC/CCL17 secretion via the suppression of STAT1 and NF-κB p65 phosphorylation in TNF-α and IFN-γ-stimulated HaCaT keratinocytes, and the release of histamine and LTC4 by suppressing 5-LO activation in PMA and A23187-stimulated MC/9 mast cells. Kuwanon G and morusin are potential anti-inflammatory mediators for the treatment of allergic and inflammatory skin diseases such as AD.

scholarly journals Experimental and clinical study of the efficacy of medicines containing omega-3 and 6 polyunsaturated fatty acids, in the treatment of inflammatory skin diseases

2018 ◽  
Vol 4 (2) ◽  
pp. 84-92
Author(s):  
Svetlana Grebennikova ◽  
Yurij Maklyakov
Keyword(s):  
Fatty Acids ◽  
Contact Dermatitis ◽  
Polyunsaturated Fatty Acids ◽  
Skin Diseases ◽  
Irritant Contact Dermatitis ◽  
Omega 3 ◽  
Inflammatory Skin Diseases ◽  
White Rats ◽  
Inflammatory Skin ◽  
Anti Inflammatory

Introduction: Over the recent years, the attention of scientists regarding the search for alternative means of treatment, including local therapy, of inflammatory skin diseases, has been focused in recent years on medicines containing omega-3 and 6 polyunsaturated fatty acids (PUFA). Objectives: to substantiate the feasibility of using medicines containing omega-3 and 6 polyunsaturated fatty acids and antioxidants in the treatment of inflammatory skin diseases. Methods:The studies were carried out on 224 conventional white rats of the Wistar line in compliance with the international principles of the European Convention for the Protection of Vertebrates. A burn injury was used to cause experimental simple irritant contact dermatitis. After trying various treatment options with using Omegaven, histopathological examinations of 18 fragments of affected skin of white rats with cross sections stained with hematoxylin–eosin and pikrofuxin by Van Gieson’s method. The state of lipid peroxidation (LPO) in blood and affected skin was evaluated in the animals. One hundred forty-six patients with inflammatory skin diseases (dermatitis simple irritant contact, allergic contact dermatitis, atopic dermatitis, neurodermatitis, psoriasis, cutaneous mastocytosis in children) were observed. In treatment, there were used medicines with omega-3 and 6 PUFA or an antioxidant medicines. Results and discussion: Skin reactions of dermatitis simple irritant contact in rats after burn injury were evaluated at different points of time. The observations showed higher efficacy of medicines containing PUFA than standard anti-inflammatory agents in the treatment of simple irritant contact dermatitis. Histopathological examination of the skin of white rats with simple irritant contact dermatitis after the 11-day treatment revealed that the expression and composition of the cellular reaction in the lesions with a predominating lymphocytes and macrophages (mainly cell response) differ significantly from those trated with Radevit ointment (segmented neutrophil leukocytes, eosinophils – delay in the acute phase of inflammation). There was identified a smaller thickness (up to 1/3) of the strips of granulation tissue under the actively proliferating cells of the epidermis. It was possible to demonstrate the superiority with respect to reducing the activity of LPO medicines containing omega-3 and 6 PUFA, over those with anti-inflammatory action. The clinical observations of patients showed high efficacy of the local treatment with medicines containing omega-3 and 6 PUFA, or antioxidant. Conclusion: For the first time, the mechanism of implementing an anti-inflammatory effect of the experimental medicines containing omega-3 and 6 polyunsaturated fatty acids (Omegaven, Vitamin F99 cream rich), – an antioxidant effect - when treating simple irritant contact dermatitis when treating simple irritant contact dermatitis; that is the weakening of the severity of oxidative stress. For the first time, the greatest contribution of an increased activity of catalase to the weakening of oxidative stress in the affected skin is shown.

scholarly journals Immunomodulatory Properties of Umbilical Cord Blood-Derived Small Extracellular Vesicles and Their Therapeutic Potential for Inflammatory Skin Disorders

2021 ◽  
Vol 22 (18) ◽  
pp. 9797
Author(s):  
Sílvia C. Rodrigues ◽  
Renato M. S. Cardoso ◽  
Patricia C. Freire ◽  
Cláudia F. Gomes ◽  
Filipe V. Duarte ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Extracellular Vesicles ◽  
Umbilical Cord Blood ◽  
Skin Diseases ◽  
Therapeutic Potential ◽  
Psoriatic Skin ◽  
Inflammatory Skin Diseases ◽  
Inflammatory Skin ◽  
Anti Inflammatory

Umbilical cord blood (UCB) has long been seen as a rich source of naïve cells with strong regenerative potential, likely mediated by paracrine signals. More recently, small extracellular vesicles (sEV), such as exosomes, have been shown to play essential roles in cell-to-cell communication, via the transport of numerous molecules, including small RNAs. Often explored for their potential as biomarkers, sEV are now known to have regenerative and immunomodulating characteristics, particularly if isolated from stem cell-rich tissues. In this study, we aim to characterize the immunomodulating properties of umbilical cord blood mononuclear cell-derived sEV (UCB-MNC-sEV) and explore their therapeutic potential for inflammatory skin diseases. UCB-MNC-sEV were shown to shift macrophages toward an anti-inflammatory phenotype, which in turn exert paracrine effects on fibroblasts, despite previous inflammatory stimuli. Additionally, the incubation of PBMC with UCB-MNC-sEV resulted in a reduction of total CD4+ and CD8+ T-cell proliferation and cytokine release, while specifically supporting the development of regulatory T-cells (Treg), by influencing FOXP3 expression. In a 3D model of psoriatic skin, UCB-MNC-sEV reduced the expression of inflammatory and psoriatic markers IL6, IL8, CXCL10, COX2, S100A7, and DEFB4. In vivo, UCB-MNC-sEV significantly prevented or reversed acanthosis in imiquimod-induced psoriasis, and tendentially increased the number of Treg in skin, without having an overall impact on disease burden. This work provides evidence for the anti-inflammatory and tolerogenic effect of UCB-MNC-sEV, which may be harnessed for the treatment of Th17-driven inflammatory skin diseases, such as psoriasis.

scholarly journals High prevalence and little awareness in patients with chronic inflammatory skin diseases and genital involvement

2021 ◽  
Author(s):  
Petra Staubach ◽  
Natascha Plavic‐Radeka ◽  
Adriane Peveling‐Oberhag ◽  
Anna Sohn ◽  
Sebastian Zimmer ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Inflammatory Skin Diseases ◽  
High Prevalence ◽  
Inflammatory Skin
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