scholarly journals Role of Estrogen Receptors α and β in a Murine Model of Asthma: Exacerbated Airway Hyperresponsiveness and Remodeling in ERβ Knockout Mice

2020 ◽  
Vol 10 ◽  
Author(s):  
Rama Satyanarayana Raju Kalidhindi ◽  
Nilesh Sudhakar Ambhore ◽  
Sangeeta Bhallamudi ◽  
Jagadish Loganathan ◽  
Venkatachalem Sathish
Keyword(s):  
Estrogen Receptors ◽  
Murine Model ◽  
Knockout Mice ◽  
Airway Hyperresponsiveness

scholarly journals The importance of leukotrienes in airway inflammation in a mouse model of asthma.

1996 ◽  
Vol 184 (4) ◽  
pp. 1483-1494 ◽  
Author(s):  
W R Henderson ◽  
D B Lewis ◽  
R K Albert ◽  
Y Zhang ◽  
W J Lamm ◽  
...  
Keyword(s):  
Mouse Model ◽  
Murine Model ◽  
Airway Hyperresponsiveness ◽  
Pulmonary Inflammation ◽  
Late Phase ◽  
Lavage Fluid ◽  
Bronchial Hyperreactivity ◽  
Leukocytic Infiltration ◽  
Specific Inhibitors

Inhalation of antigen in immunized mice induces an infiltration of eosinophils into the airways and increased bronchial hyperreactivity as are observed in human asthma. We employed a model of late-phase allergic pulmonary inflammation in mice to address the role of leukotrienes (LT) in mediating airway eosinophilia and hyperreactivity to methacholine. Allergen intranasal challenge in OVA-sensitized mice induced LTB4 and LTC4 release into the airspace, widespread mucus occlusion of the airways, leukocytic infiltration of the airway tissue and broncho-alveolar lavage fluid that was predominantly eosinophils, and bronchial hyperreactivity to methacholine. Specific inhibitors of 5-lipoxygenase and 5-lipoxygenase-activating protein (FLAP) blocked airway mucus release and infiltration by eosinophils indicating a key role for leukotrienes in these features of allergic pulmonary inflammation. The role of leukotrienes or eosinophils in mediating airway hyperresponsiveness to aeroallergen could not be established, however, in this murine model.

Diesel exhaust particulate induces airway hyperresponsiveness in a murine model: Essential role of GM-CSF☆☆☆

1999 ◽  
Vol 104 (5) ◽  
pp. 1024-1030 ◽  
Author(s):  
Ken Ohta ◽  
Naomi Yamashita ◽  
Makoto Tajima ◽  
Takashi Miyasaka ◽  
Junichi Nakano ◽  
...  
Keyword(s):  
Murine Model ◽  
Diesel Exhaust ◽  
Airway Hyperresponsiveness ◽  
Essential Role ◽  
Gm Csf ◽  
Diesel Exhaust Particulate ◽  
Exhaust Particulate

scholarly journals Exacerbation of AMD Phenotype in Lasered CNV Murine Model by Dysbiotic Oral Pathogens

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 309
Author(s):  
Pachiappan Arjunan ◽  
Radhika Swaminathan ◽  
Jessie Yuan ◽  
Mohamed Elashiry ◽  
Amany Tawfik ◽  
...  
Keyword(s):  
Murine Model ◽  
Retinal Thickness ◽  
Age Related Macular Degeneration ◽  
Periodontal Pathogen ◽  
Fundus Photography ◽  
Mouse Retina ◽  
Rrna Gene ◽  
Age Related ◽  
Periodontal Infection

Emerging evidence underscores an association between age-related macular degeneration (AMD) and periodontal disease (PD), yet the biological basis of this linkage and the specific role of oral dysbiosis caused by PD in AMD pathophysiology remains unclear. Furthermore, a simple reproducible model that emulates characteristics of both AMD and PD has been lacking. Hence, we established a novel AMD+PD murine model to decipher the potential role of oral infection (ligature-enhanced) with the keystone periodontal pathogen Porphyromonas gingivalis, in the progression of neovasculogenesis in a laser-induced choroidal-neovascularization (Li-CNV) mouse retina. By a combination of fundus photography, optical coherence tomography, and fluorescein angiography, we documented inflammatory drusen-like lesions, reduced retinal thickness, and increased vascular leakage in AMD+PD mice retinae. H&E further confirmed a significant reduction of retinal thickness and subretinal drusen-like deposits. Immunofluorescence microscopy revealed significant induction of choroidal/retinal vasculogenesis in AMD+PD mice. qPCR identified increased expression of oxidative-stress, angiogenesis, pro-inflammatory mediators, whereas antioxidants and anti-inflammatory genes in AMD+PD mice retinae were notably decreased. Through qPCR, we detected Pg and its fimbrial 16s-RrNA gene expression in the AMD+PD mice retinae. To sum-up, this is the first in vivo study signifying a role of periodontal infection in augmentation of AMD phenotype, with the aid of a pioneering AMD+PD murine model established in our laboratory.

Sign in / Sign up

Export Citation Format

Share Document