scholarly journals Ultrasound Stimulation Modulates Voltage-Gated Potassium Currents Associated With Action Potential Shape in Hippocampal CA1 Pyramidal Neurons

2019 ◽  
Vol 10 ◽  
Author(s):  
Zhengrong Lin ◽  
Xiaowei Huang ◽  
Wei Zhou ◽  
Wenjun Zhang ◽  
Yingzhe Liu ◽  
...  
Keyword(s):  
Action Potential ◽  
Pyramidal Neurons ◽  
Potassium Currents ◽  
Voltage Gated ◽  
Ca1 Pyramidal Neurons ◽  
Ultrasound Stimulation ◽  
Potential Shape ◽  
Hippocampal Ca1 ◽  
Action Potential Shape

scholarly journals Dendritic Voltage-Gated Ion Channels Regulate the Action Potential Firing Mode of Hippocampal CA1 Pyramidal Neurons

1999 ◽  
Vol 82 (4) ◽  
pp. 1895-1901 ◽  
Author(s):  
Jeffrey C. Magee ◽  
Michael Carruth
Keyword(s):  
Ion Channels ◽  
Action Potential ◽  
Large Amplitude ◽  
Action Potentials ◽  
Pyramidal Neurons ◽  
Burst Firing ◽  
Voltage Gated ◽  
Ca1 Pyramidal Neurons ◽  
Firing Mode ◽  
Voltage Gated Ion Channels

The role of dendritic voltage-gated ion channels in the generation of action potential bursting was investigated using whole cell patch-clamp recordings from the soma and dendrites of CA1 pyramidal neurons located in hippocampal slices of adult rats. Under control conditions somatic current injections evoked single action potentials that were associated with an afterhyperpolarization (AHP). After localized application of 4-aminopyridine (4-AP) to the distal apical dendritic arborization, the same current injections resulted in the generation of an afterdepolarization (ADP) and multiple action potentials. This burst firing was not observed after localized application of 4-AP to the soma/proximal dendrites. The dendritic 4-AP application allowed large-amplitude Na+-dependent action potentials, which were prolonged in duration, to backpropagate into the distal apical dendrites. No change in action potential backpropagation was seen with proximal 4-AP application. Both the ADP and action potential bursting could be inhibited by the bath application of nonspecific concentrations of divalent Ca2+ channel blockers (NiCl and CdCl). Ca2+ channel blockade also reduced the dendritic action potential duration without significantly affecting spike amplitude. Low concentrations of TTX (10–50 nM) also reduced the ability of the CA1 neurons to fire in the busting mode. This effect was found to be the result of an inhibition of backpropagating dendritic action potentials and could be overcome through the coordinated injection of transient, large-amplitude depolarizing current into the dendrite. Dendritic current injections were able to restore the burst firing mode (represented as a large ADP) even in the presence of high concentrations of TTX (300–500 μM). These data suggest the role of dendritic Na+ channels in bursting is to allow somatic/axonal action potentials to backpropagate into the dendrites where they then activate dendritic Ca2+ channels. Although it appears that most Ca2+ channel subtypes are important in burst generation, blockade of T- and R-type Ca2+ channels by NiCl (75 μM) inhibited action potential bursting to a greater extent than L-channel (10 μM nimodipine) or N-, P/Q-type (1 μM ω-conotoxin MVIIC) Ca2+ channel blockade. This suggest that the Ni-sensitive voltage-gated Ca2+ channels have the most important role in action potential burst generation. In summary, these data suggest that the activation of dendritic voltage-gated Ca2+ channels, by large-amplitude backpropagating spikes, provides a prolonged inward current that is capable of generating an ADP and burst of multiple action potentials in the soma of CA1 pyramidal neurons. Dendritic voltage-gated ion channels profoundly regulate the processing and storage of incoming information in CA1 pyramidal neurons by modulating the action potential firing mode from single spiking to burst firing.

scholarly journals Voltage-gated potassium channels ensure action potential shape fidelity in distal axons

2020 ◽  
Author(s):  
Victoria Gonzalez Sabater ◽  
Mark Rigby ◽  
Juan Burrone
Keyword(s):  
Potassium Channels ◽  
Action Potential ◽  
Hippocampal Neurons ◽  
Digital Signal ◽  
Voltage Gated ◽  
Kv Channel ◽  
Potential Shape ◽  
Blocking Voltage ◽  
Presynaptic Boutons ◽  
Action Potential Shape

The initiation and propagation of the action potential (AP) along an axon allows neurons to convey information rapidly and across distant sites. Although AP properties have typically been characterised at the soma and proximal axon, the propagation of APs towards distal axonal domains of mammalian neurons remains limited. We used Genetically Encoded Voltage Indicators (GEVIs) to image APs simultaneously at different locations along the long axons of dissociated hippocampal neurons with sub-millisecond temporal resolution. We found that APs became sharper and showed remarkable fidelity as they traveled towards distal axons, even during a high frequency train. Blocking voltage-gated potassium channels (Kv) with 4-AP resulted in an increase in AP width in all compartments, which was stronger at distal locations and exacerbated during AP trains. We conclude that the higher levels of Kv channel activity in distal axons serves to sustain AP fidelity, conveying a reliable digital signal to presynaptic boutons.

TRH regulates action potential shape in cerebral cortex pyramidal neurons

2014 ◽  
Vol 1571 ◽  
pp. 1-11 ◽  
Author(s):  
Víctor Rodríguez-Molina ◽  
Javier Patiño ◽  
Yamili Vargas ◽  
Edith Sánchez-Jaramillo ◽  
Patricia Joseph-Bravo ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Action Potential ◽  
Pyramidal Neurons ◽  
Potential Shape ◽  
Action Potential Shape

Development of Action Potential Waveform in Hippocampal CA1 Pyramidal Neurons

Neuroscience ◽  
2020 ◽  
Vol 442 ◽  
pp. 151-167 ◽  
Author(s):  
Alberto Sánchez-Aguilera ◽  
Gonzalo Monedero ◽  
Asunción Colino ◽  
María Ángeles Vicente-Torres
Keyword(s):  
Action Potential ◽  
Pyramidal Neurons ◽  
Ca1 Pyramidal Neurons ◽  
Hippocampal Ca1 ◽  
Action Potential Waveform ◽  
Potential Waveform

Voltage-gated calcium channels distribute throughout the dendrite of hippocampal CA1 pyramidal neurons

1991 ◽  
Vol 16 ◽  
pp. 4
Author(s):  
Miyakawa Hiroyoshi ◽  
David Jaffe ◽  
Daniel Johnston ◽  
John Lisman ◽  
Nechama Lasser-Ross ◽  
...  
Keyword(s):  
Calcium Channels ◽  
Pyramidal Neurons ◽  
Voltage Gated ◽  
Ca1 Pyramidal Neurons ◽  
Voltage Gated Calcium Channels ◽  
Hippocampal Ca1
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