scholarly journals Use of a Primary Epithelial Cell Screening Tool to Investigate Phage Therapy in Cystic Fibrosis

2018 ◽  
Vol 9 ◽  
Author(s):  
Stephanie Trend ◽  
Barbara J. Chang ◽  
Mark O’Dea ◽  
Stephen M. Stick ◽  
Anthony Kicic ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cell ◽  
Screening Tool ◽  
Phage Therapy ◽  
Primary Epithelial Cell

scholarly journals Human Primary Epithelial Cell Models: Promising Tools in the Era of Cystic Fibrosis Personalized Medicine

2018 ◽  
Vol 9 ◽  
Author(s):  
Nikhil T. Awatade ◽  
Sharon L. Wong ◽  
Chris K. Hewson ◽  
Laura K. Fawcett ◽  
Anthony Kicic ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cell ◽  
Personalized Medicine ◽  
Cell Models ◽  
Primary Epithelial Cell

Primary Epithelial Cell Models for Cystic Fibrosis Research

2011 ◽  
pp. 285-310 ◽  
Author(s):  
Scott H. Randell ◽  
M. Leslie Fulcher ◽  
Wanda O’Neal ◽  
John C. Olsen
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cell ◽  
Cell Models ◽  
Primary Epithelial Cell

scholarly journals 1615. Isolation of Lytic Bacteriophages with Broad Host Range Activity Against Pseudomonas aeruginosa Strains Isolated from Respiratory Samples from Cystic Fibrosis Patients Intended for Therapeutic Application

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S801-S801
Author(s):  
Jose Alexander ◽  
Daniel Navas ◽  
Marly Flowers ◽  
Angela Charles ◽  
Amy Carr
Keyword(s):  
Cystic Fibrosis ◽  
Host Range ◽  
Phage Therapy ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Plaque Morphology ◽  
Broad Host Range ◽  
Chronic Infections ◽  
Clinically Significant ◽  
Host Target

Abstract Background With the rise of the antimicrobial resistance between different genera and species of bacteria, Phage Therapy is becoming a more realistic and accessible option for patients with limited or no antimicrobial options. Being able to have rapid access to a collection of clinical active phages is key for rapid implementation of phage therapy. The Microbiology Department at AdventHealth Orlando is performing routine screening of environmental and patient samples for isolation of phages against non-fermenting Gram negative bacteria to develop a Phage Bank. Methods Protocols for phage isolation from environmental sources such as lakes, rivers and sewers and clinical samples were developed. A series of respiratory, throat, stool and urine samples were processed following an internal protocol that includes centrifugation, filtration and enrichment. Clinical samples were centrifugated for 10 minutes, filtered using 0.45µm centrifugation filters, seeded with targeted host bacteria (clinical isolates) and incubated at 35°C for 24 hours. The enriched samples were centrifugated and filtered for a final phage enriched solution. Screening and isolation were performed using the Gracia method over trypticase soybean agar (TSA) for plaque morphology and quantification. Host range screening of other clinical isolates of P. aeruginosa was performed using the new isolated and purified phages. Results 4 lytic phages against clinical strains of P. aeruginosa from patient with diagnosis of cystic fibrosis (CF), were isolated and purified from 4 different respiratory samples, including sputum and bronchial alveolar lavage. All phages showed phenotypical characteristics of lytic activity. 1 phage was active against 4 strains of P. aeruginosa, 1 phage was active against 2 strains of P. aeruginosa and the remaining 2 phages were active only against the initial host target strain. Conclusion With this study we demonstrated the potential use of clinical samples as source for isolating active bacteriophages against clinically significant bacteria strains. Clinical samples from vulnerable population of patients with chronic infections are part of our routine “phage-hunting” process to stock and grow our Phage Bank project for future clinical use. Disclosures All Authors: No reported disclosures

Establishment of primary epithelial cell culture from elutriated rat gastric mucosal cells

1995 ◽  
Vol 30 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Yoshikazu Kinoshita ◽  
Sazzad Hassan ◽  
Hirohisa Nakata ◽  
Masakyo Asahara ◽  
Yumi Matsushima ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Epithelial Cell Culture ◽  
Gastric Mucosal Cells ◽  
Primary Epithelial Cell ◽  
Mucosal Cells

Primary epithelial cell culture of adult rat kidney, enhancement of cell growth by ammonium acetate

1979 ◽  
Vol 121 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Jules Berman ◽  
Alan Perantoni ◽  
Hester Marie Jackson ◽  
Elizabeth Kingsbury
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Epithelial Cell ◽  
Ammonium Acetate ◽  
Rat Kidney ◽  
Epithelial Cell Culture ◽  
Adult Rat ◽  
Primary Epithelial Cell

scholarly journals A screening tool to identify risk for bronchiectasis progression in children with cystic fibrosis

2021 ◽  
Author(s):  
Daan Caudri ◽  
Lidija Turkovic ◽  
Nicholas H de Klerk ◽  
Tim Rosenow ◽  
Conor P. Murray ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Screening Tool
Sign in / Sign up

Export Citation Format

Share Document