scholarly journals Corrigendum: The NaV1.7 Channel Subtype as an Antinociceptive Target for Spider Toxins in Adult Dorsal Root Ganglia Neurons

2018 ◽  
Vol 9 ◽  
Author(s):  
Tânia C. Gonçalves ◽  
Evelyne Benoit ◽  
Michel Partiseti ◽  
Denis Servent
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Spider Toxins ◽  
Dorsal Root Ganglia Neurons

scholarly journals Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

2021 ◽  
Vol 22 (11) ◽  
pp. 5499
Author(s):  
Veronica Corsetti ◽  
Carla Perrone-Capano ◽  
Michael Sebastian Salazar Intriago ◽  
Elisabetta Botticelli ◽  
Giancarlo Poiana ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Splice Variants ◽  
Multiple Roles ◽  
Pcr Analysis ◽  
Drg Neurons ◽  
Embryonic Developmental Stage ◽  
Embryo Stage ◽  
Dorsal Root Ganglia Neurons ◽  
Cholinergic Markers

Dorsal root ganglia (DRG) neurons synthesize acetylcholine (ACh), in addition to their peptidergic nature. They also release ACh and are cholinoceptive, as they express cholinergic receptors. During gangliogenesis, ACh plays an important role in neuronal differentiation, modulating neuritic outgrowth and neurospecific gene expression. Starting from these data, we studied the expression of choline acetyltransferase (ChAT) and vesicular ACh transporter (VAChT) expression in rat DRG neurons. ChAT and VAChT genes are arranged in a “cholinergic locus”, and several splice variants have been described. Using selective primers, we characterized splice variants of these cholinergic markers, demonstrating that rat DRGs express R1, R2, M, and N variants for ChAT and V1, V2, R1, and R2 splice variants for VAChT. Moreover, by RT-PCR analysis, we observed a progressive decrease in ChAT and VAChT transcripts from the late embryonic developmental stage (E18) to postnatal P2 and P15 and in the adult DRG. Interestingly, Western blot analyses and activity assays demonstrated that ChAT levels significantly increased during DRG ontogenesis. The modulated expression of different ChAT and VAChT splice variants during development suggests a possible differential regulation of cholinergic marker expression in sensory neurons and confirms multiple roles for ACh in DRG neurons, both in the embryo stage and postnatally.

Cryopreservation of transfected primary dorsal root ganglia neurons

2008 ◽  
Vol 173 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Angela M. Seggio ◽  
Karen S. Ellison ◽  
Matthew R. Hynd ◽  
William Shain ◽  
Deanna M. Thompson
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Dorsal Root Ganglia Neurons

scholarly journals Characteristics of Dorsal Root Ganglia Neurons Sensitive to Substance P

2014 ◽  
Vol 10 ◽  
pp. 1744-8069-10-73 ◽  
Author(s):  
Eder Ricardo Moraes ◽  
Christopher Kushmerick ◽  
Ligia Araujo Naves
Keyword(s):  
Substance P ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Dorsal Root Ganglia Neurons
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