scholarly journals Beta Amyloid Differently Modulate Nicotinic and Muscarinic Receptor Subtypes which Stimulate in vitro and in vivo the Release of Glycine in the Rat Hippocampus

2012 ◽  
Vol 3 ◽  
Author(s):  
Stefania Zappettini ◽  
Massimo Grilli ◽  
Guendalina Olivero ◽  
Elisa Mura ◽  
Stefania Preda ◽  
...  
Keyword(s):  
Muscarinic Receptor ◽  
Beta Amyloid ◽  
Rat Hippocampus ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes

In Vivo Characterization of Muscarinic Receptor Subtypes That Mediate Vasodilatation in Patients With Essential Hypertension

Hypertension ◽  
1995 ◽  
Vol 26 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Tobias A. Bruning ◽  
Peter C. Chang ◽  
Maarten G.C. Hendriks ◽  
Pieter Vermeij ◽  
Martin Pfaffendorf ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
In Vivo Characterization

M1 and M3 muscarinic antagonists inhibit human nasal glandular secretion in vitro

1992 ◽  
Vol 73 (5) ◽  
pp. 2069-2073 ◽  
Author(s):  
J. Mullol ◽  
J. N. Baraniuk ◽  
C. Logun ◽  
M. Merida ◽  
J. Hausfeld ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Muscarinic Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Receptor Subtypes ◽  
Muscarinic Antagonists ◽  
Muscarinic Receptor Subtypes ◽  
Mucus Production ◽  
Glandular Secretion ◽  
M3 Receptor

Mucus glycoproteins (MGP) are high-molecular-weight glycoconjugates that are released from submucosal glands and epithelial goblet cells in the respiratory tract. Muscarinic receptors have an important role in the regulation of human nasal glandular secretion and mucus production, but it is not known which of the five muscarinic receptor subtypes are involved. The effect of nonselective and M1-, M2-, and M3-selective muscarinic antagonists on methacholine (MCh)-induced MGP secretion from human nasal mucosal explants was tested in vitro. MGP was assayed by enzyme-linked immunosorbent assay using a specific anti-MGP monoclonal antibody (7F10). MCh (100 microM) induced MGP secretion up to 127% compared with controls. MCh-induced MGP release was significantly inhibited by atropine (100 microM), the M, receptor antagonist pirenzepine (10–100 microM), and the M3 receptor antagonist 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP; 1–100 microM). 4-DAMP significantly inhibited MCh-induced MGP release at a lower concentration (1 microM) than pirenzepine (10 microM). The M2 receptor antagonists AF-DX 116 and gallamine (both at 100 microM) had no effect. No antagonist alone had a significant effect on MGP release. These results indicate that the M1 and M3 muscarinic receptor subtypes regulate MGP secretion from human nasal mucosa and suggest that the M3 receptor has the predominant effect.

Differential inflammatory modulation of canine ileal longitudinal and circular muscle cells

1999 ◽  
Vol 277 (2) ◽  
pp. G341-G350 ◽  
Author(s):  
Xuan-Zheng Shi ◽  
Sushil K. Sarna
Keyword(s):  
Receptor Antagonist ◽  
Muscarinic Receptor ◽  
Longitudinal Muscle ◽  
Circular Muscle ◽  
Muscle Cells ◽  
Muscle Contractions ◽  
Receptor Subtypes ◽  
Relative Role

The aim of this study was to identify the subtypes of muscarinic receptors that mediate in vivo and in vitro canine ileal longitudinal muscle contractions and whether their role is modulated by inflammation. Previous studies have reported that circular muscle contractions are suppressed in ileal inflammation induced by mucosal exposure to ethanol and acetic acid. We found that inflammation had no significant effect on in vivo and in vitro spontaneous or muscarinic receptor-mediated contractions of the longitudinal muscle. The longitudinal muscle contractions were mediated primarily by the M3 receptor subtype. However, the IC50 of the M2 receptor antagonist methoctramine was only 10 times greater than that of the M3 receptor antagonist 4-DAMP in the longitudinal muscle, whereas it was 224 times greater in the circular muscle. M2receptor-coupled decrease of intracellular cAMP occurred in the longitudinal but not in the circular muscle from the normal ileum. Inflammation did not alter this coupling in the longitudinal muscle but established it in the circular muscle. In conclusion, M2 receptors may play a greater role in the mediation of longitudinal muscle contractions than circular muscle contractions. Inflammation does not alter the contractility or the relative role of muscarinic receptor subtypes in longitudinal muscle cells. However, it modulates the M2 receptor coupling to adenylate cyclase in the circular muscle.

In vivo characterization of muscarinic receptor subtypes that mediate vasodilatation in patients with essential hypertension

Life Sciences ◽  
1995 ◽  
Vol 56 (11-12) ◽  
pp. 1049
Author(s):  
T.A. Bruning ◽  
P.C. Chang ◽  
M.G.C. Hendriks ◽  
P. Vermeij ◽  
M. Pfaffendorf ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
In Vivo Characterization

scholarly journals The interaction of hexamethonium with muscarinic receptor subtypes in vitro

1989 ◽  
Vol 98 (2) ◽  
pp. 499-506 ◽  
Author(s):  
R.M. Eglen ◽  
A.D. Michel ◽  
C.M. Cornett ◽  
E.A. Kunysz ◽  
R.L. Whiting
Keyword(s):  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes

Novel oxotremorine-related heterocyclic derivatives: Synthesis and in vitro pharmacology at the muscarinic receptor subtypes

2007 ◽  
Vol 15 (24) ◽  
pp. 7626-7637 ◽  
Author(s):  
Clelia Dallanoce ◽  
Marco De Amici ◽  
Elisabetta Barocelli ◽  
Simona Bertoni ◽  
Bryan L. Roth ◽  
...  
Keyword(s):  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Heterocyclic Derivatives ◽  
In Vitro Pharmacology

Muscarinic receptor subtypes in canine trachea

1990 ◽  
Vol 258 (6) ◽  
pp. L349-L354 ◽  
Author(s):  
J. F. Brichant ◽  
D. O. Warner ◽  
S. J. Gunst ◽  
K. Rehder
Keyword(s):  
Electric Field ◽  
Muscarinic Receptor ◽  
Contractile Response ◽  
Receptor Subtypes ◽  
Field Stimulation ◽  
Muscarinic Receptor Subtypes ◽  
Electric Field Stimulation ◽  
M3 Receptors ◽  
M3 Subtype

Prejunctional and postjunctional muscarinic receptor subtypes were characterized in canine trachealis muscle strips. In vitro contractile responses of muscle strips to acetylcholine or electric field stimulation were determined in the absence and the presence of gallamine, pirenzepine, and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP). Gallamine had no effect on the contractile response to acetylcholine but enhanced the contractile response to electric field stimulation. Pirenzepine and 4-DAMP reduced the contractile response to acetylcholine and electric field stimulation. The pA2 value for pirenzepine vs. acetylcholine [7.18 +/- 0.59 (SD)] was consistent with the affinity of pirenzepine for M2 or M3-receptors; whereas the pA2 value for 4-DAMP vs. acetylcholine (8.92 +/- 0.42) and the extremely low affinity of gallamine indicated postjunctional muscarinic receptors of the M3 subtype. The enhancement of the contractile response to electric field stimulation by gallamine suggested the presence of M2-prejunctional receptors.

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