scholarly journals Correlation Between TNFAIP2 Gene Polymorphism and Prediction/Prognosis for Gastric Cancer and Its Effect on TNFAIP2 Protein Expression

2020 ◽  
Vol 10 ◽  
Author(s):  
Fang Guo ◽  
Qian Xu ◽  
Zhi Lv ◽  
Han-Xi Ding ◽  
Li-Ping Sun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gene Polymorphism ◽  
Protein Expression

scholarly journals Cannabidiol Induces Cell Cycle Arrest and Cell Apoptosis in Human Gastric Cancer SGC-7901 Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 302 ◽  
Author(s):  
Xin Zhang ◽  
Yao Qin ◽  
Zhaohai Pan ◽  
Minjing Li ◽  
Xiaona Liu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Cycle ◽  
Protein Expression ◽  
Cell Cycle Arrest ◽  
Phase Cell ◽  
G1 Phase ◽  
Therapeutic Effects ◽  
Human Gastric Cancer ◽  
Expression Levels ◽  
Cycle Arrest

The main chemical component of cannabis, cannabidiol (CBD), has been shown to have antitumor properties. The present study examined the in vitro effects of CBD on human gastric cancer SGC-7901 cells. We found that CBD significantly inhibited the proliferation and colony formation of SGC-7901 cells. Further investigation showed that CBD significantly upregulated ataxia telangiectasia-mutated gene (ATM) and p53 protein expression and downregulated p21 protein expression in SGC-7901 cells, which subsequently inhibited the levels of CDK2 and cyclin E, thereby resulting in cell cycle arrest at the G0–G1 phase. In addition, CBD significantly increased Bax expression levels, decreased Bcl-2 expression levels and mitochondrial membrane potential, and then upregulated the levels of cleaved caspase-3 and cleaved caspase-9, thereby inducing apoptosis in SGC-7901 cells. Finally, we found that intracellular reactive oxygen species (ROS) increased after CBD treatment. These results indicated that CBD could induce G0–G1 phase cell cycle arrest and apoptosis by increasing ROS production, leading to the inhibition of SGC-7901 cell proliferation, thereby suggesting that CBD may have therapeutic effects on gastric cancer.

scholarly journals Identification of differential proteomics in Epstein-Barr virus-associated gastric cancer and related functional analysis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zeyang Wang ◽  
Zhi Lv ◽  
Qian Xu ◽  
Liping Sun ◽  
Yuan Yuan
Keyword(s):  
Gastric Cancer ◽  
Functional Analysis ◽  
Protein Expression ◽  
Epstein Barr Virus ◽  
Protein Profile ◽  
Expression Patterns ◽  
Clinicopathological Parameters ◽  
Differential Proteomics ◽  
Barr Virus ◽  
Epstein Barr

Abstract Background Epstein-Barr virus-associated gastric cancer (EBVaGC) is the most common EBV-related malignancy. A comprehensive research for the protein expression patterns in EBVaGC established by high-throughput assay remains lacking. In the present study, the protein profile in EBVaGC tissue was explored and related functional analysis was performed. Methods Epstein-Barr virus-encoded RNA (EBER) in situ hybridization (ISH) was applied to EBV detection in GC cases. Data-independent acquisition (DIA) mass spectrometry (MS) was performed for proteomics assay of EBVaGC. Functional analysis of identified proteins was conducted with bioinformatics methods. Immunohistochemistry (IHC) staining was employed to detect protein expression in tissue. Results The proteomics study for EBVaGC was conducted with 7 pairs of GC cases. A total of 137 differentially expressed proteins in EBV-positive GC group were identified compared with EBV-negative GC group. A PPI network was constructed for all of them, and several proteins with relatively high interaction degrees could be the hub genes in EBVaGC. Gene enrichment analysis showed they might be involved in the biological pathways related to energy and biochemical metabolism. Combined with GEO datasets, a highly associated protein (GBP5) with EBVaGC was screened out and validated with IHC staining. Further analyses demonstrated that GBP5 protein might be associated with clinicopathological parameters and EBV infection in GC. Conclusions The newly identified proteins with significant differences and potential central roles could be applied as diagnostic markers of EBVaGC. Our study would provide research clues for EBVaGC pathogenesis as well as novel targets for the molecular-targeted therapy of EBVaGC.

scholarly journals Comparison of the protein expression of calpain-1, calpain-2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa

2017 ◽  
Vol 14 (3) ◽  
pp. 3705-3710 ◽  
Author(s):  
Bide Liu ◽  
Yu Zhou ◽  
Dan Lu ◽  
Yong Liu ◽  
Si-Quan Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Mucosa ◽  
Protein Expression ◽  
Normal Gastric Mucosa ◽  
Calpain 2

The Association Between XPG Gene Polymorphism and Gastric Cancer Risk

2017 ◽  
Vol 21 (10) ◽  
pp. 619-624 ◽  
Author(s):  
Yanyan Su ◽  
Chong Yang ◽  
Zongxiang Zhang
Keyword(s):  
Gastric Cancer ◽  
Cancer Risk ◽  
Gene Polymorphism ◽  
Gastric Cancer Risk

scholarly journals A Non-canonical Wnt Signature Correlates With Lower Survival in Gastric Cancer

2021 ◽  
Vol 9 ◽  
Author(s):  
Pablo Astudillo
Keyword(s):  
Gastric Cancer ◽  
Protein Expression ◽  
Wnt Signaling Pathway ◽  
Mesenchymal Cell ◽  
Gene And Protein Expression ◽  
Cancer Initiation ◽  
Wnt Pathways ◽  
Canonical Wnt ◽  
Mesenchymal Cell Marker ◽  
Shared Gene

Genetic evidence suggests a role for the Wnt/β-catenin pathway in gastric cancer. However, Wnt5a, regarded as a prototypical non-canonical Wnt ligand, has also been extensively associated with this disease. Therefore, the roles of the Wnt signaling pathway in gastric cancer initiation and progression, and particularly the precise mechanisms by which the non-canonical Wnt pathway might promote the development and progression of gastric cancer, are not entirely well understood. This article analyzes publicly available gene and protein expression data and reveals the existence of a WNT5A/FZD2/FZD7/ROR2 signature, which correlates with tumor-infiltrating and mesenchymal cell marker expression. High expression of FZD7 and ROR2 correlates with a shared gene and protein expression profile, which in turn correlates with poor prognosis. In summary, the findings presented in this article provide an updated view of the relative contributions of the Wnt/β-catenin and non-canonical Wnt pathways in gastric cancer.

scholarly journals Endo-sulfatase Sulf-1 Protein Expression is Down-regulated in Gastric Cancer

2012 ◽  
Vol 13 (2) ◽  
pp. 641-646 ◽  
Author(s):  
Gopisetty Gopal ◽  
Sundersingh Shirley ◽  
Uthandaraman Mahalinga Raja ◽  
Thangarajan Rajkumar
Keyword(s):  
Gastric Cancer ◽  
Protein Expression

scholarly journals IL-1B (C+3954T) Gene Polymorphism and Susceptibility to Gastric Cancer in the Iranian Population

2015 ◽  
Vol 16 (2) ◽  
pp. 841-844 ◽  
Author(s):  
Ahmad Ismaili ◽  
Kheirollah Yari ◽  
Mohammad-Taher Moradi ◽  
Maryam Sohrabi ◽  
Danial Kahrizi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gene Polymorphism ◽  
Iranian Population
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