scholarly journals A Novel Bacteriocin From Lactobacillus Pentosus ZFM94 and Its Antibacterial Mode of Action

2021 ◽  
Vol 8 ◽  
Author(s):  
Mengdi Dai ◽  
Yanran Li ◽  
Luyao Xu ◽  
Danli Wu ◽  
Qingqing Zhou ◽  
...  
Keyword(s):  
High Performance ◽  
Micrococcus Luteus ◽  
Gel Chromatography ◽  
Lactobacillus Pentosus ◽  
Minimal Inhibitory Concentrations ◽  
Flight Mass Spectrometry ◽  
Lipid Ii ◽  
Heat Stable ◽  
Wide Ph Range ◽  
Ph Range

Bacteriocins are bioactive antimicrobial peptides synthesized in the ribosome of numerous bacteria and released extracellularly. Pentocin ZFM94 produced by Lactobacillus pentosus (L. pentosus) ZFM94, isolated from infant feces with strong antibacterial activity, was purified by ammonium sulfate precipitation, dextran gel chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The molecular mass of the purified bacteriocin was 3,547.74 Da determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Pentocin ZFM94 exhibited broad-spectrum antimicrobial activity against tested Gram-positive and Gram-negative bacteria, and the minimal inhibitory concentrations (MICs) of Micrococcus luteus (M. luteus) 10,209, Staphylococcus aureus (S. aureus) D48, and Escherichia coli (E. coli) DH5α were 1.75, 2.00, and 2.50 μm, respectively. Pentocin ZFM94 was heat-stable (30 min at 80°C) and showed inhibitory activity over a wide pH range (5.00–7.00). It could be degraded by trypsin and pepsin, but not by amylase, lysozyme, lipase, and ribonuclease A. Fluorescence leakage assay showed that pentocin ZFM94 induced disruption of the cell membrane and caused leakage of cellular content. Furthermore, lipid II was not an antibacterial target of pentocin ZFM94. This study laid the foundation for further development and utilization of L. pentosus ZFM94 and its bacteriocin.

NiMo/Cu-nanosheets/Ni-foam composite as a high performance electrocatalyst for hydrogen evolution over a wide pH range

2021 ◽  
Vol 46 (5) ◽  
pp. 3493-3503
Author(s):  
Zhuangzhuang Chang ◽  
Lianjie Zhu ◽  
Jian Zhao ◽  
Peiwen Chen ◽  
Deyou Chen ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
High Performance ◽  
Ni Foam ◽  
Foam Composite ◽  
Wide Ph Range ◽  
Ph Range

ZIF-mediated N-doped hollow porous carbon as a high performance adsorbent for tetracycline removal from water with wide pH range

2020 ◽  
Vol 182 ◽  
pp. 109059 ◽  
Author(s):  
Chunhong Liang ◽  
Yue Tang ◽  
Xiaodan Zhang ◽  
Hongxiang Chai ◽  
Yuming Huang ◽  
...  
Keyword(s):  
Porous Carbon ◽  
High Performance ◽  
Wide Ph Range ◽  
Ph Range

scholarly journals Preparation and Evaluation of New Glycopeptides Obtained by Proteolysis from Corn Gluten Meal Followed by Transglutaminase-Induced Glycosylation with Glucosamine

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 555
Author(s):  
Xiao-Lan Liu ◽  
Chun-Li Song ◽  
Jia-Peng Chen ◽  
Xiang Liu ◽  
Jian Ren ◽  
...  
Keyword(s):  
High Performance ◽  
Surface Hydrophobicity ◽  
Dependent Manner ◽  
Ros Scavenging ◽  
Corn Gluten Meal ◽  
Food Ingredient ◽  
Flight Mass Spectrometry ◽  
Corn Gluten ◽  
Ph Range

New glycopeptides were generated by proteolysis from corn gluten meal (CGM) followed by transglutaminase (TGase)-induced glycosylation with glucosamine (GlcN). The glycopeptides exhibited desirable antioxidant and intracellular ROS-scavenging properties. The amount of conjugated GlcN quantified by high-performance liquid chromatography (HPLC) was 23.0 g/kg protein. The formed glycopeptides contained both glycosylated and glycation types, as demonstrated by the electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS/MS). The glycopeptides exhibited scavenging capabilities against free radical diphenylpicrylhydrazyl (DPPH) and hydroxyl radicals by reducing their power. The potential protection of glycopeptides against ethanol-induced injury in LO2 cells was assessed In Vitro based on methyl thiazole tetrazolium (MTT) testing and intracellular reactive oxygen species (ROS) scavenging capacity, respectively. Glycopeptide cytoprotection was expressed in a dose-dependent manner, with the glycopeptides exhibiting good solubility ranging from 74.8% to 83.2% throughout a pH range of 2–10. Correspondingly, the glycopeptides showed good emulsifying activity (36.0 m2/g protein), emulsion stability (74.9%), and low surface hydrophobicity (16.3). These results indicate that glycosylation of CGM significantly improved its biological and functional properties. Glycopeptides from CGM could be used as potential antioxidants as well as comprising a functional food ingredient.

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