scholarly journals Model-Predicted Balance Between Neural Excitation and Inhibition Was Maintained Despite of Age-Related Decline in Sensory Evoked Local Field Potential in Rat Barrel Cortex

2020 ◽  
Vol 14 ◽  
Author(s):  
Sungmin Kang ◽  
Yurie Hayashi ◽  
Michael Bruyns-Haylett ◽  
Evangelos Delivopoulos ◽  
Ying Zheng
Keyword(s):  
Local Field ◽  
Local Field Potential ◽  
Barrel Cortex ◽  
Field Potential ◽  
Age Related ◽  
Neural Excitation ◽  
Sensory Evoked

scholarly journals Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

2021 ◽  
Vol 15 ◽  
Author(s):  
Claudia Cecchetto ◽  
Stefano Vassanelli ◽  
Bernd Kuhn
Keyword(s):  
Local Field ◽  
Calcium Imaging ◽  
Local Field Potential ◽  
Barrel Cortex ◽  
Field Potential ◽  
Imaging Data ◽  
Spatiotemporal Resolution ◽  
Two Photon ◽  
Cranial Window ◽  
Sensory Evoked

Neuronal population activity, both spontaneous and sensory-evoked, generates propagating waves in cortex. However, high spatiotemporal-resolution mapping of these waves is difficult as calcium imaging, the work horse of current imaging, does not reveal subthreshold activity. Here, we present a platform combining voltage or calcium two-photon imaging with multi-channel local field potential (LFP) recordings in different layers of the barrel cortex from anesthetized and awake head-restrained mice. A chronic cranial window with access port allows injecting a viral vector expressing GCaMP6f or the voltage-sensitive dye (VSD) ANNINE-6plus, as well as entering the brain with a multi-channel neural probe. We present both average spontaneous activity and average evoked signals in response to multi-whisker air-puff stimulations. Time domain analysis shows the dependence of the evoked responses on the cortical layer and on the state of the animal, here separated into anesthetized, awake but resting, and running. The simultaneous data acquisition allows to compare the average membrane depolarization measured with ANNINE-6plus with the amplitude and shape of the LFP recordings. The calcium imaging data connects these data sets to the large existing database of this important second messenger. Interestingly, in the calcium imaging data, we found a few cells which showed a decrease in calcium concentration in response to vibrissa stimulation in awake mice. This system offers a multimodal technique to study the spatiotemporal dynamics of neuronal signals through a 3D architecture in vivo. It will provide novel insights on sensory coding, closing the gap between electrical and optical recordings.

Reconstruction of intracortical whisker-evoked local field potential from electrocorticogram using a model trained for spontaneous activity in the rat barrel cortex

2014 ◽  
Vol 87 ◽  
pp. 40-48 ◽  
Author(s):  
Hidenori Watanabe ◽  
Tomoya Sakatani ◽  
Takafumi Suzuki ◽  
Masa-aki Sato ◽  
Yukio Nishimura ◽  
...  
Keyword(s):  
Spontaneous Activity ◽  
Local Field ◽  
Local Field Potential ◽  
Barrel Cortex ◽  
Field Potential

Vagus Nerve Stimulation Induced Synchrony Modulation of Local Field Potential in the Rat Cerebral Cortex

2013 ◽  
Vol 133 (8) ◽  
pp. 1493-1500 ◽  
Author(s):  
Ryuji Kano ◽  
Kenichi Usami ◽  
Takahiro Noda ◽  
Tomoyo I. Shiramatsu ◽  
Ryohei Kanzaki ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Vagus Nerve ◽  
Local Field ◽  
Nerve Stimulation ◽  
Local Field Potential ◽  
Vagus Nerve Stimulation ◽  
Field Potential ◽  
Rat Cerebral Cortex

Exploiting the directional local field potential recording capability of a new DBS lead for functional targeting in Parkinson’s disease

2015 ◽  
Vol 8 (2) ◽  
pp. 380
Author(s):  
M.A.J. Lourens ◽  
M.F. Contarino ◽  
R. Verhagen ◽  
P. van den Munckhof ◽  
P.R. Schuurman ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Local Field ◽  
Local Field Potential ◽  
Field Potential ◽  
Potential Recording ◽  
Field Potential Recording

Cultured olfactory interneurons from Limax maximus: optical and electrophysiological studies of transmitter-evoked responses

1993 ◽  
Vol 69 (6) ◽  
pp. 1940-1947 ◽  
Author(s):  
L. D. Rhines ◽  
P. G. Sokolove ◽  
J. Flores ◽  
D. W. Tank ◽  
A. Gelperin
Keyword(s):  
Action Potential ◽  
Intracellular Calcium ◽  
Local Field ◽  
Local Field Potential ◽  
Field Potential ◽  
Optical Measurements ◽  
Optical Studies ◽  
Isolated Cells ◽  
Fura 2 ◽  
Limax Maximus

1. The olfactory processing network in the procerebral (PC) lobe of the terrestrial mollusk Limax maximus exhibits a coherent oscillation of local field potential that is modulated by odor input. To understand the cellular basis of this oscillation, we developed a cell culture preparation of isolated PC neurons and studied the responses of isolated cells to stimulation with neurotransmitters known to be present in the PC lobe. 2. The distribution of PC soma diameters suggests at least two different populations of neurons. Approximately 95% of isolated cells had soma diameters of 7-8 microns, with the remaining cells having larger diameters (10-15 microns). 3. Extracellular measurements of action potentials and optical measurements of intracellular calcium concentrations in fura-2-loaded cells were made. Serotonin and dopamine excited PC neurons and promoted transitions from steady to bursty activity. Both amines elicited increases in intracellular calcium, presumably concomitant with the increase in action-potential frequency. 4. Glutamate suppressed action-potential firing and reduced intracellular calcium. This effect was seen most clearly when glutamate was applied to cells excited by high potassium medium. Quisqualate is an effective glutamate agonist in this system, whereas kainate is not. 5. Combined with anatomic and biochemical data and with studies of the effects of these neurotransmitters on the oscillating local field potential of the intact PC network, the data from isolated PC neurons are consistent with the hypothesis that dopamine and serotonin modulate network dynamics, whereas glutamate is involved in generating the basic oscillation of local field potential in the PC. 6. The optical studies of fura-2-loaded cells showed that several treatments that increase the rate of action-potential production lead to elevations in intracellular calcium. Optical studies of intracellular calcium may be useful for multisite measurements of activity in the intact, oscillating PC lobe network.

scholarly journals Increased neural correlations in primate auditory cortex during slow-wave sleep

2013 ◽  
Vol 109 (11) ◽  
pp. 2732-2738 ◽  
Author(s):  
Elias B. Issa ◽  
Xiaoqin Wang
Keyword(s):  
Auditory Cortex ◽  
Spontaneous Activity ◽  
Slow Wave ◽  
Local Field ◽  
Local Field Potential ◽  
Slow Wave Sleep ◽  
Field Potential ◽  
Sensory Stimulation ◽  
Acoustic Stimulation ◽  
Functional Connections

During sleep, changes in brain rhythms and neuromodulator levels in cortex modify the properties of individual neurons and the network as a whole. In principle, network-level interactions during sleep can be studied by observing covariation in spontaneous activity between neurons. Spontaneous activity, however, reflects only a portion of the effective functional connectivity that is activated by external and internal inputs (e.g., sensory stimulation, motor behavior, and mental activity), and it has been shown that neural responses are less correlated during external sensory stimulation than during spontaneous activity. Here, we took advantage of the unique property that the auditory cortex continues to respond to sounds during sleep and used external acoustic stimuli to activate cortical networks for studying neural interactions during sleep. We found that during slow-wave sleep (SWS), local (neuron-neuron) correlations are not reduced by acoustic stimulation remaining higher than in wakefulness and rapid eye movement sleep and remaining similar to spontaneous activity correlations. This high level of correlations during SWS complements previous work finding elevated global (local field potential-local field potential) correlations during sleep. Contrary to the prediction that slow oscillations in SWS would increase neural correlations during spontaneous activity, we found little change in neural correlations outside of periods of acoustic stimulation. Rather, these findings suggest that functional connections recruited in sound processing are modified during SWS and that slow rhythms, which in general are suppressed by sensory stimulation, are not the sole mechanism leading to elevated network correlations during sleep.

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