scholarly journals Synaptic Vesicle Proteins and Active Zone Plasticity

Author(s):  
Robert J. Kittel ◽  
Manfred Heckmann
2014 ◽  
Vol 11 (10) ◽  
pp. 971-980 ◽  
Author(s):  
Melanie Laßek ◽  
Jens Weingarten ◽  
Amparo Acker-Palmer ◽  
Sandra Bajjalieh ◽  
Ulrike Muller ◽  
...  

2020 ◽  
Author(s):  
Torsten W. B. Götz ◽  
Dmytro Puchkov ◽  
Janine Lützkendorf ◽  
Alexander G. Nikonenko ◽  
Christine Quentin ◽  
...  

SummaryReliable delivery of presynaptic material, including active zone and synaptic vesicle proteins from neuronal somata to synaptic terminals is prerequisite for faithful synaptogenesis and neurotransmission. However, molecular mechanisms controlling the somatic assembly of presynaptic precursors remain insufficiently understood. Here we show that in mutants of the small GTPase RAB2 active zone and synaptic vesicle proteins accumulated in the neuronal somata at the trans-Golgi network and were consequently depleted at synaptic terminals, provoking neurotransmission deficits. The ectopic presynaptic material accumulations consisted of heterogeneous vesicles and short tubules of 40×60 nm and segregated in subfractions either positive for active zone proteins or co-positive for synaptic vesicle proteins and LAMP1, a lysosomal membrane protein. Genetically, rab2 behaved epistatic over arl8, a lysosomal adaptor controlling axonal export of precursors. Collectively, we here identified a Golgi-associated assembly sequence in presynaptic precursor vesicle biogenesis controlled by RAB2 dependent membrane remodelling and protein sorting at the trans-Golgi.


2021 ◽  
Vol 220 (5) ◽  
Author(s):  
Torsten W.B. Götz ◽  
Dmytro Puchkov ◽  
Veronika Lysiuk ◽  
Janine Lützkendorf ◽  
Alexander G. Nikonenko ◽  
...  

Reliable delivery of presynaptic material, including active zone and synaptic vesicle proteins from neuronal somata to synaptic terminals, is prerequisite for successful synaptogenesis and neurotransmission. However, molecular mechanisms controlling the somatic assembly of presynaptic precursors remain insufficiently understood. We show here that in mutants of the small GTPase Rab2, both active zone and synaptic vesicle proteins accumulated in the neuronal cell body at the trans-Golgi and were, consequently, depleted at synaptic terminals, provoking neurotransmission deficits. Ectopic presynaptic material accumulations consisted of heterogeneous vesicles and short tubules of 40 × 60 nm, segregating in subfractions either positive for active zone or synaptic vesicle proteins and LAMP1, a lysosomal membrane protein. Genetically, Rab2 acts upstream of Arl8, a lysosomal adaptor controlling axonal export of precursors. Collectively, we identified a Golgi-associated assembly sequence of presynaptic precursor biogenesis dependent on a Rab2-regulated protein export and sorting step at the trans-Golgi.


2021 ◽  
Vol 559 ◽  
pp. 92-98
Author(s):  
Judyta K. Juranek ◽  
Konark Mukherjee ◽  
Reinhard Jahn ◽  
Jia-Yi Li

2011 ◽  
Vol 108 (25) ◽  
pp. E222-E229 ◽  
Author(s):  
F. Kawasaki ◽  
J. Iyer ◽  
L. L. Posey ◽  
C. E. Sun ◽  
S. E. Mammen ◽  
...  

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