scholarly journals Estimation of presynaptic calcium currents and endogenous calcium buffers at the frog neuromuscular junction with two different calcium fluorescent dyes

2015 ◽  
Vol 6 ◽  
Author(s):  
Dmitry Samigullin ◽  
Nijaz Fatikhov ◽  
Eduard Khaziev ◽  
Andrey Skorinkin ◽  
Eugeny Nikolsky ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Fluorescent Dyes ◽  
Calcium Currents ◽  
Frog Neuromuscular Junction ◽  
Calcium Buffers ◽  
Presynaptic Calcium

scholarly journals Acetylcholine-Induced Inhibition of Presynaptic Calcium Signals and Transmitter Release in the Frog Neuromuscular Junction

2016 ◽  
Vol 7 ◽  
Author(s):  
Eduard Khaziev ◽  
Dmitry Samigullin ◽  
Nikita Zhilyakov ◽  
Nijaz Fatikhov ◽  
Ellya Bukharaeva ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Transmitter Release ◽  
Frog Neuromuscular Junction ◽  
Calcium Signals ◽  
Presynaptic Calcium

scholarly journals Transmitter release is evoked with low probability predominately by calcium flux through single channel openings at the frog neuromuscular junction

2015 ◽  
Vol 113 (7) ◽  
pp. 2480-2489 ◽  
Author(s):  
Fujun Luo ◽  
Markus Dittrich ◽  
Soyoun Cho ◽  
Joel R. Stiles ◽  
Stephen D. Meriney
Keyword(s):  
Neuromuscular Junction ◽  
Calcium Channels ◽  
Calcium Channel ◽  
Calcium Ions ◽  
Transmitter Release ◽  
Synaptic Vesicles ◽  
Vesicle Fusion ◽  
Frog Neuromuscular Junction ◽  
Low Probability ◽  
Presynaptic Calcium

The quantitative relationship between presynaptic calcium influx and transmitter release critically depends on the spatial coupling of presynaptic calcium channels to synaptic vesicles. When there is a close association between calcium channels and synaptic vesicles, the flux through a single open calcium channel may be sufficient to trigger transmitter release. With increasing spatial distance, however, a larger number of open calcium channels might be required to contribute sufficient calcium ions to trigger vesicle fusion. Here we used a combination of pharmacological calcium channel block, high-resolution calcium imaging, postsynaptic recording, and 3D Monte Carlo reaction-diffusion simulations in the adult frog neuromuscular junction, to show that release of individual synaptic vesicles is predominately triggered by calcium ions entering the nerve terminal through the nearest open calcium channel. Furthermore, calcium ion flux through this channel has a low probability of triggering synaptic vesicle fusion (∼6%), even when multiple channels open in a single active zone. These mechanisms work to control the rare triggering of vesicle fusion in the frog neuromuscular junction from each of the tens of thousands of individual release sites at this large model synapse.

221 Presynaptic Ca2+-imaging at the frog neuromuscular junction

1996 ◽  
Vol 25 ◽  
pp. S36 ◽  
Author(s):  
M. Osanai ◽  
S. Suzuki ◽  
N. Suzuki ◽  
K. Kuba ◽  
H. Kijima
Keyword(s):  
Neuromuscular Junction ◽  
Frog Neuromuscular Junction
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