scholarly journals Sex and Feeding Status Differently Affect Natural Reward Seeking Behavior in Olfactory Bulbectomized Rats

2018 ◽  
Vol 12 ◽  
Author(s):  
Jana Ruda-Kucerova ◽  
Mary Tresa Zanda ◽  
Petra Amchova ◽  
Walter Fratta ◽  
Liana Fattore
Keyword(s):  
Natural Reward ◽  
Reward Seeking ◽  
Seeking Behavior

Reward-Seeking Behavior and Addiction: Cause or Cog?

2012 ◽  
Vol 5 (3) ◽  
pp. 178-189 ◽  
Author(s):  
Oscar Arias-Carrion ◽  
Mohamed Salama
Keyword(s):  
Reward Seeking ◽  
Seeking Behavior

Cyclohexane Inhalation Produces Long-Lasting Alterations in the Hippocampal Integrity and Reward-Seeking Behavior in the Adult Mouse

2019 ◽  
Vol 39 (3) ◽  
pp. 435-449 ◽  
Author(s):  
Tania Campos-Ordonez ◽  
David Zarate-Lopez ◽  
Nereida Ibarra-Castaneda ◽  
Jonathan Buritica ◽  
Oscar Gonzalez-Perez
Keyword(s):  
Adult Mouse ◽  
Reward Seeking ◽  
Seeking Behavior

scholarly journals The Female Urine Sniffing Test: A Novel Approach for Assessing Reward-Seeking Behavior in Rodents

2010 ◽  
Vol 67 (9) ◽  
pp. 864-871 ◽  
Author(s):  
Oz Malkesman ◽  
Maria Luisa Scattoni ◽  
Daniel Paredes ◽  
Tyson Tragon ◽  
Brandon Pearson ◽  
...  
Keyword(s):  
Reward Seeking ◽  
Novel Approach ◽  
Female Urine ◽  
Seeking Behavior

scholarly journals Thermostabilization and purification of the human dopamine transporter (hDAT) in an inhibitor and allosteric ligand bound conformation

2018 ◽  
Author(s):  
Vikas Navratna ◽  
Dilip K. Tosh ◽  
Kenneth A. Jacobson ◽  
Eric Gouaux
Keyword(s):  
Dopamine Transporter ◽  
Limiting Factor ◽  
Molecular Architecture ◽  
Secondary Active Transport ◽  
Reward Seeking ◽  
Dopamine Reuptake ◽  
Seeking Behavior ◽  
Chemical Messenger ◽  
Hormonal Release ◽  
Detergent Micelles

AbstractThe human dopamine transporter(hDAT) plays a major role in dopamine homeostasis and regulation of neurotransmission by clearing dopamine from the extracellular space using secondary active transport. Dopamine is an essential monoamine chemical messenger that regulates reward seeking behavior, motor control, hormonal release, and emotional response in humans. Psychostimulants such as cocaine primarily target the central binding site of hDAT and lock the transporter in an outward-facing conformation, thereby inhibiting dopamine reuptake. The inhibition of dopamine reuptake leads to accumulation of dopamine in the synapse causing heightened signaling. In addition, hDAT is implicated in various neurological disorders and disease-associated neurodegeneration. Despite its significance, the molecular architecture of hDAT and its various conformational states are poorly understood. Instability of hDAT in detergent micelles has been a limiting factor in its successful biochemical, biophysical, and structural characterization. To overcome this hurdle, first we identified ligands that stabilize hDAT in detergent micelles. Then, we screened ∼200 single residue mutants of hDAT using high-throughput scintillation proximity assay, and identified a thermostable variant(I248Y). Here we report a robust strategy to overexpress and successfully purify a thermostable variant of hDAT in an inhibitor and allosteric ligand bound conformation.

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