scholarly journals Functionality of In vitro Reconstituted Group II Intron RmInt1-Derived Ribonucleoprotein Particles

2016 ◽  
Vol 3 ◽  
Author(s):  
Maria D. Molina-Sánchez ◽  
Fernando M. García-Rodríguez ◽  
Nicolás Toro
Keyword(s):  
Group Ii Intron ◽  
Ribonucleoprotein Particles ◽  
Group Ii

scholarly journals Methylation of rRNA as a host defense against rampant group II intron retrotransposition

Mobile DNA ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Justin M. Waldern ◽  
Dorie Smith ◽  
Carol Lyn Piazza ◽  
E. Jake Bailey ◽  
Nicholas J. Schiraldi ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Fold Increase ◽  
Group Ii Intron ◽  
In Vivo Experiments ◽  
Cellular Factors ◽  
Group Ii ◽  
Self Splicing ◽  
Native Host

Abstract Background Group II introns are mobile retroelements, capable of invading new sites in DNA. They are self-splicing ribozymes that complex with an intron-encoded protein to form a ribonucleoprotein that targets DNA after splicing. These molecules can invade DNA site-specifically, through a process known as retrohoming, or can invade ectopic sites through retrotransposition. Retrotransposition, in particular, can be strongly influenced by both environmental and cellular factors. Results To investigate host factors that influence retrotransposition, we performed random insertional mutagenesis using the ISS1 transposon to generate a library of over 1000 mutants in Lactococcus lactis, the native host of the Ll.LtrB group II intron. By screening this library, we identified 92 mutants with increased retrotransposition frequencies (RTP-ups). We found that mutations in amino acid transport and metabolism tended to have increased retrotransposition frequencies. We further explored a subset of these RTP-up mutants, the most striking of which is a mutant in the ribosomal RNA methyltransferase rlmH, which exhibited a reproducible 20-fold increase in retrotransposition frequency. In vitro and in vivo experiments revealed that ribosomes in the rlmH mutant were defective in the m3Ψ modification and exhibited reduced binding to the intron RNA. Conclusions Taken together, our results reinforce the importance of the native host organism in regulating group II intron retrotransposition. In particular, the evidence from the rlmH mutant suggests a role for ribosome modification in limiting rampant retrotransposition.

scholarly journals Group II intron domain 5 facilitates a trans-splicing reaction.

1988 ◽  
Vol 8 (6) ◽  
pp. 2361-2366 ◽  
Author(s):  
K A Jarrell ◽  
R C Dietrich ◽  
P S Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Intron Sequence ◽  
Trans Splicing ◽  
Group Ii ◽  
Self Splicing ◽  
Yeast Mitochondrial Dna ◽  
Domain 4

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

scholarly journals Influence of substrate structure on in vitro ribozyme activity of a group II intron

RNA ◽  
1998 ◽  
Vol 4 (6) ◽  
pp. 694-708 ◽  
Author(s):  
ALEXIS NOLTE ◽  
GUILLAUME CHANFREAU ◽  
ALAIN JACQUIER
Keyword(s):  
Group Ii Intron ◽  
Substrate Structure ◽  
Group Ii ◽  
Influence Of Substrate

scholarly journals DNA polymerization catalysed by a group II intron RNA in vitro

1997 ◽  
Vol 25 (9) ◽  
pp. 1825-1829 ◽  
Author(s):  
M. Hetzer ◽  
R. J. Schweyen ◽  
M. W. Mueller
Keyword(s):  
Group Ii Intron ◽  
Dna Polymerization ◽  
Group Ii

GAPDH enhances group II intron splicing in vitro

2004 ◽  
Vol 385 (7) ◽  
Author(s):  
P. Böck-Taferner ◽  
H. Wank
Keyword(s):  
Group Ii Intron ◽  
Group Ii Introns ◽  
Intron Splicing ◽  
Group Ii

AbstractGroup II introns are autocatalytic RNAs which selfsplice

scholarly journals ATP-Dependent Roles of the DEAD-Box Protein Mss116p in Group II Intron Splicing In Vitro and In Vivo

2011 ◽  
Vol 411 (3) ◽  
pp. 661-679 ◽  
Author(s):  
Jeffrey P. Potratz ◽  
Mark Del Campo ◽  
Rachel Z. Wolf ◽  
Alan M. Lambowitz ◽  
Rick Russell
Keyword(s):  
Group Ii Intron ◽  
Intron Splicing ◽  
Dead Box ◽  
The Dead ◽  
Group Ii
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