scholarly journals Bacillus velezensis CLA178-Induced Systemic Resistance of Rosa multiflora Against Crown Gall Disease

2020 ◽  
Vol 11 ◽  
Author(s):  
Lin Chen ◽  
Xinghong Wang ◽  
Qinghua Ma ◽  
Lusen Bian ◽  
Xue Liu ◽  
...  
Keyword(s):  
Crown Gall ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Rosa Multiflora ◽  
Bacillus Velezensis ◽  
Crown Gall Disease

scholarly journals Identification of miRNAs Involved in Bacillus velezensis FZB42-Activated Induced Systemic Resistance in Maize

2019 ◽  
Vol 20 (20) ◽  
pp. 5057 ◽  
Author(s):  
Shanshan Xie ◽  
Hengguo Yu ◽  
Enze Li ◽  
Yu Wang ◽  
Juan Liu ◽  
...  
Keyword(s):  
Defense Response ◽  
Target Genes ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Abiotic Stress Response ◽  
Bacillus Velezensis ◽  
Nuclear Factor Y ◽  
Network Analyses ◽  
Cis Element ◽  
Differentially Expressed Mirna

Bacillus velezensis FZB42 is able to activate induced systemic resistance (ISR) to enhance plant defense response against pathogen infections. Though the roles of microRNAs (miRNAs) in Bacillus-triggered ISR have been reported in Arabidopsis, the maize miRNAs responsible for the Bacillus-activated ISR process have not been discovered. To explore the maize miRNAs involved in ISR, maize miRNAs in response to FZB42 (ISR activating), FZB42△sfp△alss (deficient in triggering ISR), and a control for 12 h were sequenced. A total of 146 known miRNAs belonging to 30 miRNA families and 217 novel miRNAs were identified. Four miRNAs specifically repressed in FZB42-treatment were selected as candidate ISR-associated miRNAs. All of them contained at least one defense response-related cis-element, suggesting their potential roles in activating the ISR process. Interestingly, three of the four candidate ISR-associated miRNAs belong to the conserved miR169 family, which has previously been confirmed to play roles in abiotic stress response. Moreover, 52 mRNAs were predicted as potential targets of these candidate ISR-associated miRNAs through TargetFinder software and degradome sequencing. Gene Ontology (GO) and network analyses of target genes showed that these differentially expressed miRNA might participate in the ISR process by regulating nuclear factor Y transcription factor. This study is helpful in better understanding the regulatory roles of maize miRNAs in the Bacillus-activated ISR process.

scholarly journals Characterization of Migration and Induced Resistance of Rhizobium Vitis Strain ARK-1, a Biological Control Agent Against Grapevine Crown Gall Disease

2020 ◽  
Author(s):  
Akira Kawaguchi ◽  
Yoshiteru Noutoshi
Keyword(s):  
Crown Gall ◽  
Temporal Dynamics ◽  
Biological Control Agent ◽  
Control Agent ◽  
Systemic Resistance ◽  
Marker Genes ◽  
Cell Detection ◽  
Induced Expression ◽  
Crown Gall Disease ◽  
Grapevine Crown Gall

Abstract A nonpathogenic strain ARK-1 of Rhizobium vitis has an antagonistic activity toward tumorigenic (Ti) strain of R. vitis, a causal agent of grapevine crown gall disease, and works as a biocontrol agent. We have demonstrated that the gall formation was fully suppressed when ARK-1 was co-inoculated with Ti into the grapevine stem at a 1:1 ratio. For practical use of ARK-1 in agriculture, understanding the temporal dynamics of the bacterial habitat on host plants and the biocontrol property are needed in order to develop proper application methods. Here we demonstrated that the gall incidence by Ti was reduced to about 50% when ARK-1 was pre-inoculated at both upper and lower positions on the grapevine stem 3 cm away from the Ti-inoculation point 5 days before. The bacterial cell detection assay in the grapevine tissue revealed that ARK-1 could migrate at least 3 cm in 5 days. Inoculations of ARK-1 or Ti induced expression of marker genes for defense-related phytohormones such as salicylic acid, jasmonic acid, and ethylene in grapevine within 3 days but they were diminished by 6 days. Inoculation of Ti 5 days after ARK-1 pre-inoculation induced expression of the marker genes except for the LOX-9 gene in a basically similar way to those without the pre-inoculation, suggesting that ARK-1 did not induce typical acquired systemic resistance or induced systemic resistance in grapevine, while the transcript of LOX-9 was detected at 24 and 48 hours after the Ti inoculation when ARK-1 was pre-inoculated, unlike the un-inoculated condition. ARK-1 primed the induction of certain defense genes and it may take part in its biocontrol activity.

scholarly journals Bacillus velezensis strain MBY2, a potential agent for the management of crown gall disease

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252823
Author(s):  
Haifa Ben Gharsa ◽  
Meriam Bouri ◽  
Amira Mougou Hamdane ◽  
Christina Schuster ◽  
Andreas Leclerque ◽  
...  
Keyword(s):  
Crown Gall ◽  
Pathogenic Bacteria ◽  
Screening Program ◽  
Plant Protection ◽  
Antagonistic Activity ◽  
Soil Conditions ◽  
Bacillus Velezensis ◽  
Crown Gall Disease

The reduction of the use chemical pesticides in agriculture is gaining importance as an objective of decision-makers in both politics and economics. Consequently, the development of technically efficient and economically affordable alternatives as, e.g., biological control agents or practices is highly solicited. Crown gall disease of dicotyledonous plants is caused by ubiquitous soil borne pathogenic bacteria of the Agrobacterium tumefaciens species complex, that comprises the species Agrobacterium fabrum and represents a globally relevant plant protection problem. Within the framework of a screening program for bacterial Agrobacterium antagonists a total of 14 strains were isolated from Tunisian soil samples and assayed for antagonistic activity against pathogenic agrobacteria. One particularly promising isolate, termed strain MBY2, was studied more in depth. Using a Multilocus Sequence Analysis (MLSA) approach, the isolate was assigned to the taxonomic species Bacillus velezensis. Strain MBY2 was shown to display antagonistic effects against the pathogenic A. fabrum strain C58 in vitro and to significantly decrease pathogen populations under sterile and non-sterile soil conditions as well as in the rhizosphere of maize and, to a lower extent, tomato plants. Moreover, the ability of B. velezensis MBY2 to reduce C58-induced gall development has been demonstrated in vivo on stems of tomato and almond plants. The present study describes B. velezensis MBY2 as a newly discovered strain holding potential as a biological agent for crown gall disease management.

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