scholarly journals Combination of Trace Metal to Improve Solventogenesis of Clostridium carboxidivorans P7 in Syngas Fermentation

2020 ◽  
Vol 11 ◽  
Author(s):  
Yi-Fan Han ◽  
Bin-Tao Xie ◽  
Guang-xun Wu ◽  
Ya-Qiong Guo ◽  
De-Mao Li ◽  
...  
Keyword(s):  
Trace Metal ◽  
Syngas Fermentation ◽  
Clostridium Carboxidivorans

scholarly journals Effect of temperature and surfactant on biomass growth and higher-alcohol production during syngas fermentation by Clostridium carboxidivorans P7

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Shaohuang Shen ◽  
Guan Wang ◽  
Ming Zhang ◽  
Yin Tang ◽  
Yang Gu ◽  
...  
Keyword(s):  
Tween 80 ◽  
Biofuel Production ◽  
Effect Of Temperature ◽  
Biomass Growth ◽  
Syngas Fermentation ◽  
Higher Alcohol ◽  
Alcohol Production ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Clostridium Carboxidivorans

Abstract Hexanol–butanol–ethanol fermentation from syngas by Clostridium carboxidivorans P7 is a promising route for biofuel production. However, bacterial agglomeration in the culture of 37 °C severely hampers the accumulation of biomass and products. To investigate the effect of culture temperature on biomass growth and higher-alcohol production, C. carboxidivorans P7 was cultivated at both constant and two-step temperatures in the range from 25 to 37 °C. Meanwhile, Tween-80 and saponin were screened out from eight surfactants to alleviate agglomeration at 37 °C. The results showed that enhanced higher-alcohol production was contributed mainly by the application of two-step temperature culture rather than the addition of anti-agglomeration surfactants. Furthermore, comparative transcriptome revealed that although 37 °C promoted high expression of genes involved in the Wood–Ljungdahl pathway, genes encoding enzymes catalyzing acyl-condensation reactions associated with higher-alcohol production were highly expressed at 25 °C. This study gained greater insight into temperature-effect mechanism on syngas fermentation by C. carboxidivorans P7.

scholarly journals Batch Syngas Fermentation by Clostridium carboxidivorans for Production of Acids and Alcohols

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1075
Author(s):  
Fabiana Lanzillo ◽  
Giacomo Ruggiero ◽  
Francesca Raganati ◽  
Maria Elena Russo ◽  
Antonio Marzocchella
Keyword(s):  
Critical Role ◽  
Volume Ratio ◽  
Liquid Volume ◽  
Syngas Fermentation ◽  
Co Concentration ◽  
Depletion Rate ◽  
Growth Differences ◽  
Gas Volume ◽  
Clostridium Carboxidivorans ◽  
Kinetics Of

Syngas (CO, CO2, and H2) has attracted special attention due to the double benefit of syngas fermentation for carbon sequestration (pollution reduction), while generating energy. Syngas can be either produced by gasification of biomasses or as a by-product of industrial processes. Only few microorganisms, mainly clostridia, were identified as capable of using syngas as a substrate to produce medium chain acids, or alcohols (such as butyric acid, butanol, hexanoic acid, and hexanol). Since CO plays a critical role in the availability of reducing equivalents and carbon conversion, this work assessed the effects of constant CO partial pressure (PCO), ranging from 0.5 to 2.5 atm, on cell growth, acid production, and solvent production, using Clostridium carboxidivorans. Moreover, this work focused on the effect of the liquid to gas volume ratio (VL/VG) on fermentation performances; in particular, two VL/VG were considered (0.28 and 0.92). The main results included—(a) PCO affected the growth kinetics of the microorganism; indeed, C. carboxidivorans growth rate was characterized by CO inhibition within the investigated range of CO concentration, and the optimal PCO was 1.1 atm (corresponding to a dissolved CO concentration of about 25 mg/L) for both VL/VG used; (b) growth differences were observed when the gas-to-liquid volume ratio changed; mass transport phenomena did not control the CO uptake for VL/VG = 0.28; on the contrary, the experimental CO depletion rate was about equal to the transport rate in the case of VL/VG = 0.92.

scholarly journals Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans

Catalysts ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 59 ◽  
Author(s):  
Carolina Benevenuti ◽  
Alanna Botelho ◽  
Roberta Ribeiro ◽  
Marcelle Branco ◽  
Adejanildo Pereira ◽  
...  
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Biomass Gasification ◽  
Culture Collection ◽  
Medium Composition ◽  
Syngas Fermentation ◽  
The Cost ◽  
Clostridium Carboxidivorans ◽  
Peptone Yeast Extract Glucose

Fermentation of gases from biomass gasification, named syngas, is an important alternative process to obtain biofuels. Sequential experimental designs were used to increase cell growth and ethanol production during syngas fermentation by Clostridium carboxidivorans. Based on ATCC (American Type Culture Collection) 2713 medium composition, it was possible to propose a best medium composition for cell growth, herein called TYA (Tryptone-Yeast extract-Arginine) medium and another one for ethanol production herein called TPYGarg (Tryptone-Peptone-Yeast extract-Glucose-Arginine) medium. In comparison to ATCC® 2713 medium, TYA increased cell growth by 77%, reducing 47% in cost and TPYGarg increased ethanol production more than four-times, and the cost was reduced by 31%. In 72 h of syngas fermentation in TPYGarg medium, 1.75-g/L of cells, 2.28 g/L of ethanol, and 0.74 g/L of butanol were achieved, increasing productivity for syngas fermentation.

Butanol and hexanol production in Clostridium carboxidivorans syngas fermentation: Medium development and culture techniques

2015 ◽  
Vol 190 ◽  
pp. 114-121 ◽  
Author(s):  
John R. Phillips ◽  
Hasan K. Atiyeh ◽  
Ralph S. Tanner ◽  
Juan R. Torres ◽  
Jyotisna Saxena ◽  
...  
Keyword(s):  
Fermentation Medium ◽  
Syngas Fermentation ◽  
Culture Techniques ◽  
Medium Development ◽  
Clostridium Carboxidivorans

scholarly journals Studies on Syngas Fermentation With Clostridium carboxidivorans in Stirred-Tank Reactors With Defined Gas Impurities

2021 ◽  
Vol 12 ◽  
Author(s):  
Anton Rückel ◽  
Jens Hannemann ◽  
Carolin Maierhofer ◽  
Alexander Fuchs ◽  
Dirk Weuster-Botz
Keyword(s):  
Ethanol Concentration ◽  
Biomass Concentration ◽  
Product Formation ◽  
The Other ◽  
Stirred Tank ◽  
Syngas Fermentation ◽  
Trace Impurities ◽  
Gas Fermentation ◽  
Other Hand ◽  
Clostridium Carboxidivorans

Syngas fermentation processes with acetogenic bacteria like Clostridium carboxidivorans have been proven to be a promising approach for the conversion of CO-rich waste gases into short- and medium-chain alcohols. The challenge of synthesis gas impurities, on the other hand, has always been a major concern for establishing an industrial-scale process, since some of the trace components in waste gases, such as NH3, H2S, and NOx, can have inhibiting or even toxic effects on microbial growth and product formation. Thus, this study aims to identify the effects of the main trace impurities in syngas from gasification of biogenic residues by the supply of defined concentrations of trace impurities to the cultivation medium. Autotrophic gas fermentation studies were performed with C. carboxidivorans in batch-operated fully-controlled stirred-tank bioreactors with continuous gas supply (80% CO and 20% CO2). The syngas components NH3 and H2S had a positive effect on both growth and alcohol formation (ethanol, 1-butanol, and 1-hexanol). The maximum biomass concentration was increased by more than 50%, and the maximum ethanol concentration was more than doubled with 5.0 g L−1 NH4Cl or 1.0 g L−1 H2S provided by the addition of 2.2 g L−1 thioacetamide. The addition of the nitrogen oxide species nitrate and nitrite, on the other hand, reduced biomass growth as well as alcohol concentrations. Already, the supply of 0.1 g L−1 NaNO3 resulted in reduced growth and 25% reduction of the maximum ethanol concentration. The production of the longer chain alcohols 1-butanol and 1-hexanol was reduced as well. All NaNO2 concentrations tested showed a strong toxic effect on the metabolism of C. carboxidivorans, and neither CO consumption nor product formation was observed after addition. As a consequence, NOx components in syngas from the gasification of biogenic residues should be reduced by the gasification process and/or selectively removed from the syngas after gasification.

scholarly journals Residual Gas for Ethanol Production by Clostridium carboxidivorans in a Dual Impeller Stirred Tank Bioreactor (STBR)

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 199 ◽  
Author(s):  
Carolina Benevenuti ◽  
Marcelle Branco ◽  
Mariana do Nascimento-Correa ◽  
Alanna Botelho ◽  
Tatiana Ferreira ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Ethanol Production ◽  
Culture Medium ◽  
Biofuel Production ◽  
Stirred Tank ◽  
Low Flow ◽  
Syngas Fermentation ◽  
Stirred Tank Bioreactor ◽  
Industrial Gases ◽  
Clostridium Carboxidivorans

Recycling residual industrial gases and residual biomass as substrates to biofuel production by fermentation is an important alternative to reduce organic wastes and greenhouse gases emission. Clostridium carboxidivorans can metabolize gaseous substrates as CO and CO2 to produce ethanol and higher alcohols through the Wood-Ljungdahl pathway. However, the syngas fermentation is limited by low mass transfer rates. In this work, a syngas fermentation was carried out in serum glass bottles adding different concentrations of Tween® 80 in ATCC® 2713 culture medium to improve gas-liquid mass transfer. We observed a 200% increase in ethanol production by adding 0.15% (v/v) of the surfactant in the culture medium and a 15% increase in biomass production by adding 0.3% (v/v) of the surfactant in the culture medium. The process was reproduced in stirred tank bioreactor with continuous syngas low flow, and a maximum ethanol productivity of 0.050 g/L.h was achieved.

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